LOCUS       sequence-365767-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A5_STOP
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1595..1694)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1711..1730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1712..1730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1735..1751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1735..1751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1853..1872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1864..2673
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1931..1950)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2820..3408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3309..3328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3562..3579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3738..3765)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3857..3943)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3992..4014
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4006..4023
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4007..4023
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag gagccccgtg
      121 cgcgacctgg caaggaacga tggcgaggag tccaccgaca gaacaccact gctgccagga
      181 gcacctaggg cagaggcagc acccgtgtgc tgtagcgcca gatacaacct ggccatcctg
      241 gccttctttg gcttctttat cgtgtatgcc ctgagggtga atctgagcgt ggccctggtg
      301 gacatggtgg attccaacac cacactggag gacaatcgca cctctaaggc ctgccctgag
      361 cacagcgccc caatcaaggt gcaccacaat cagacaggca agaagtacca gtgggatgcc
      421 gagacccagg gctggatcct gggctctttc ttttacggct atatcatcac acagatccca
      481 ggcggctatg tggcctccaa gatcggcggc aagatgctgc tgggattcgg aatcctggga
      541 accgccgtgc tgaccctgtt tacaccaatc gccgccgacc tgggcgtggg ccccctgatc
      601 gtgctgcgcg ccctggaggg actgggagag ggagtgacat tccctgccat gcacgcaatg
      661 tggagctcct gggcaccacc tctggagcgg agcaagctgc tgtccatctc ttacgcagga
      721 gcacagctgg gaaccgtgat cagcctgcca ctgtccggca tcatctgtta ctatatgaac
      781 tggacatacg tgttttattt ctttggcacc atcggcatct tctggtttct gctgtggatc
      841 tggctggtgt ccgatacccc ccagaagcac aagcgcatct ctcactacga gaaggagtat
      901 atcctgtcta gcctgcggaa ccagctgtcc tctcagaagt ctgtgccatg ggtgcccatc
      961 ctgaagagcc tgcctctgtg ggcaatcgtg gtggcacact tctcctacaa ttggaccttt
     1021 tatacactgc tgaccctgct gcctacctac atgaaggaga tcctgagatt caacgtgcag
     1081 gagaatggct ttctgagctc cctgccatat ctgggctcct ggctgtgcat gatcctgtct
     1141 ggccaggccg ccgataacct gagggccaag tggaacttca gcaccctgtg cgtgcggaga
     1201 atcttttctc tgatcggcat gatcggacca gccgtgttcc tggtggcagc aggctttatc
     1261 ggctgcgact acagcctggc cgtggccttc ctgaccatct ccaccacact gggcggcttc
     1321 tgttctagcg gcttttctat caaccacctg gatatcgccc caagctatgc cggcatcctg
     1381 ctgggcatca ccaatacatt cgccacaatc cccggcatgg tgggccctgt gatcgccaag
     1441 agcctgacac ccgacaacac cgtgggcgag tggcagacag tgttttacat cgccgccgcc
     1501 atcaacgtgt tcggcgccat cttctttacc ctgtttgcca agggcgaggt gcagaactgg
     1561 gccctgaatg atcaccacgg ccaccggcac taagacccag ctttcttgta caaagttggc
     1621 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1681 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1741 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1801 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1861 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1921 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1981 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2041 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2101 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2161 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2221 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2281 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2341 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2401 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2461 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2521 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2581 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2641 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2701 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2761 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3661 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3721 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3781 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3841 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     3901 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     3961 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4021 agtcttaagc tcgggccc
//
