LOCUS       sequence-365770-        4203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A11_STOP
FEATURES             Location/Qualifiers
     source          1..4203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1760..1859)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1876..1895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1877..1895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1900..1916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1900..1916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2018..2037)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2029..2838
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2096..2115)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2985..3573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3474..3493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3727..3744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3903..3930)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4022..4108)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4157..4179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4171..4188
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4172..4188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc ctttagcaag
      121 ctgctggagc aggcaggagg agtgggactg ttccagacac tgcaggtgct gacctttatc
      181 ctgccttgcc tgatgatccc atcccagatg ctgctggaga acttctctgc cgccatccca
      241 ggccaccggt gttggacaca catgctggat aacggctctg ccgtgagcac aaatatgacc
      301 cccaaggccc tgctgaccat ctccatccca cctggaccaa accagggacc acaccagtgc
      361 cggcggttcc ggcagccaca gtggcagctg ctggacccta atgccacagc cacctcttgg
      421 agcgaggccg ataccgagcc ttgcgtggac ggctgggtgt acgatcggag cgtgttcacc
      481 agcaccatcg tggccaagtg ggacctggtg tgcagctccc agggcctgaa gccactgtcc
      541 cagtctatct tcatgagcgg catcctggtg ggctccttta tctggggcct gctgagctac
      601 cggttcggca gaaagcccat gctgtcctgg tgctgtctgc agctggcagt ggcaggcaca
      661 agcaccatct tcgcccctac atttgtgatc tattgcggcc tgagattcgt ggccgccttt
      721 ggcatggccg gcatcttcct gtctagcctg accctgatgg tggagtggac cacaacctcc
      781 aggcgcgccg tgacaatgac cgtggtggga tgtgcctttt ctgccggaca ggccgccctg
      841 ggaggactgg ccttcgccct gagggactgg cgcacactgc agctggcagc aagcgtgccc
      901 ttctttgcca tctccctgat ctcttggtgg ctgcctgagt ccgccaggtg gctgatcatc
      961 aagggcaagc cagatcaggc cctgcaggag ctgaggaagg tggcaagaat caacggccac
     1021 aaggaggcca agaatctgac catcgaggtg ctgatgtcct ctgtgaagga ggaggtggcc
     1081 tctgccaagg agccaagaag cgtgctggat ctgttttgcg tgcccgtgct gaggtggcgc
     1141 tcctgtgcca tgctggtggt gaacttctcc ctgctgatct cttactatgg cctggtgttt
     1201 gacctgcagt ctctgggccg ggatatcttc ctgctgcagg ccctgttcgg agcagtggac
     1261 tttctgggca gagccacaac cgccctgctg ctgtcttttc tgggccggag aacaatccag
     1321 gcaggaagcc aggcaatggc aggactggcc atcctggcca atatgctggt gcctcaggat
     1381 ctgcagaccc tgagggtggt gttcgccgtg ctgggcaagg gctgctttgg catctccctg
     1441 acatgtctga ccatctacaa ggccgagctg ttccctacac cagtgaggat gaccgccgac
     1501 ggcatcctgc acaccgtggg ccgcctggga gcaatgatgg gacctctgat cctgatgtct
     1561 aggcaggccc tgccactgct gccaccactg ctgtatggcg tgatcagcat cgccagctcc
     1621 ctggtggtgc tgttctttct gccagagaca cagggactgc cactgcctga caccatccag
     1681 gatctggaga gccagaagtc caccgcagca cagggaaata ggcaggaggc agtgacagtg
     1741 gagagcacct ccctgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     1801 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1861 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1921 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1981 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2041 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2101 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2161 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2221 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2281 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2341 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2401 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2461 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2521 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2581 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2641 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2701 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2761 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2821 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2881 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     2941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3121 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3601 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3661 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3721 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3781 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3841 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3901 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3961 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4021 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4081 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4141 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4201 ccc
//
