LOCUS       sequence-365772-        4242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A6_STOP
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1799..1898)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1915..1934)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1916..1934)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1939..1955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1939..1955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2057..2076)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2068..2877
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2135..2154)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3513..3532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3766..3783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3942..3969)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4061..4147)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4196..4218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4210..4227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4211..4227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cttcaacgac
      121 ctgctgcagc aagtgggagg agtgggccgg tttcagcaga tccaggtgac cctggtggtg
      181 ctgcctctgc tgctgatggc cagccacaac accctgcaga atttcacagc cgccatccca
      241 acccaccact gcaggccacc tgcagacgca aacctgtcca agaatggagg actggaagtg
      301 tggctgccta gggatagaca gggccagcca gagagctgtc tgagattcac atccccacag
      361 tggggcctgc cctttctgaa cggaaccgag gcaaatggaa ccggagcaac agagccatgc
      421 acagacggct ggatctacga taattctacc ttccctagca ccatcgtgac agagtgggac
      481 ctggtgtgca gccacagggc cctgaggcag ctggcacagt ccctgtacat ggtgggcgtg
      541 ctgctgggcg ccatggtgtt tggctatctg gccgataggc tgggccggag aaaggtgctg
      601 atcctgaact acctgcagac agccgtgtcc ggaacctgcg cagccttcgc ccctaatttt
      661 ccaatctatt gtgccttcag gctgctgtct ggaatggcac tggcaggcat cagcctgaac
      721 tgcatgaccc tgaatgtgga gtggatgcct atccacacaa gggcatgcgt gggcaccctg
      781 atcggatacg tgtattccct gggacagttt ctgctggcag gagtggcata cgcagtgcct
      841 cactggaggc acctgcagct gctggtgtct gccccattct ttgccttctt tatctattct
      901 tggttcttta tcgagagcgc cagatggcac agctcctctg gccggctgga cctgacactg
      961 agagccctgc agcgggtggc cagaatcaac ggcaagaggg aggagggagc aaagctgtcc
     1021 atggaggtgc tgagagcctc tctgcagaag gagctgacca tgggcaaggg acaggcaagc
     1081 gccatggagc tgctgaggtg cccaacactg cgccacctgt tcctgtgcct gagcatgctg
     1141 tggttcgcca cctcctttgc ctactatggc ctggtcatgg acctgcaggg cttcggcgtg
     1201 tctatctacc tgatccaggt catctttggc gccgtggatc tgccagccaa gctggtgggc
     1261 ttcctggtca tcaacagcct gggcaggcgc cccgcacaga tggccgccct gctgctggca
     1321 ggaatctgca tcctgctgaa tggcgtgatc ccccaggatc agtccatcgt gaggacatct
     1381 ctggccgtgc tgggcaaggg atgcctggca gcctccttca actgtatctt tctgtacacc
     1441 ggcgagctgt atcccacaat gatcaggcag accggcatgg gcatgggctc tacaatggca
     1501 agggtgggaa gcatcgtgtc ccctctggtg tccatgaccg ccgagctgta cccctctatg
     1561 cctctgttca tctatggagc agtgccagtg gcagcatctg ccgtgacagt gctgctgcca
     1621 gagaccctgg gacagccact gccagacaca gtgcaggatc tggagagcag atgggcacca
     1681 acccagaagg aggcaggcat ctaccctagg aagggcaagc agacccgcca gcagcaggag
     1741 caccagaagt atatggtgcc actgcaggca tccgcccagg agaagaatgg cctgtaagac
     1801 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1861 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1921 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1981 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     2041 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2101 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2161 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2221 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2281 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2341 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2401 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2461 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2521 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2581 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2641 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2701 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2761 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2821 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2881 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2941 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3841 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3901 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3961 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     4021 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     4081 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     4141 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4201 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
