LOCUS       sequence-365773-        8471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent oxytocin(OT) control 
            sensor GRAB_OTmut in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Qian T, Wang H, Wang P, Geng L, Mei L, Osakada T, Wang L, Tang Y, 
            Kania A, Grinevich V, Stoop R, Lin D, Luo M, Li Y
  TITLE     A genetically encoded sensor measures temporal oxytocin release from
            different neuronal compartments.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2.
  PUBMED    36593404
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2."
COMMENT     Sequence Label: pDisplay-GRAB_OTmut
FEATURES             Location/Qualifiers
     source          1..8471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1750..1771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1877..1898)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2138..2157)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2670..3243,3244..3246,3247..3255)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2837..2854)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3063..3082
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3256..3963
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3401..3419)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3669..3688
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4021..4050
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4054..4200
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4222..4239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4228..4452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4584..5172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4664..4683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5501..5548
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5573..5592
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5780..6574)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5891..5910)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6501..6520
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6673..6692)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6771..6962
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6919..6939
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7022..7813,7814..7882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7645..7664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7883..7987)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8014..8469
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8101..8120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8311..8332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgagggtgc
      841 gtttgcggct aactggagcg ctgaggcggt caacgggagc gcggcgccgc cgggaaccga
      901 gggcaatcgc actgccgggc cgccacagcg caacgaggcc ctggcgcggg tggaggtggc
      961 cgtgctgtgc ctcatcctgt tcctggcgct gagcggcaac gcgtgcgtgc tgctagcgct
     1021 gcgcaccacg cgccacaagc actcgcgcct cttcttcttc atgaagcacc tgagcatagc
     1081 cgacctggta gtggcggtgt tccaggtgct gccgcagctt ctgtgggaca tcacgttccg
     1141 cttctacggg cccgacctgc tgtgccgcct cgtcaagtac ctgcaggttg tgggcatgtt
     1201 cgcgtccacc tacctgctgc tgctcatgtc gctcgaccgc tgcctggcca tctgccagcc
     1261 gctgcgctcg ctgagccgcc gcaccgaccg cctggcggta ctcgtcacat ggctcggctg
     1321 cctggtggcc agcgcgccgc aggtgcacat cttctcgctg cgcgaggtgg ccgacggtgt
     1381 cttcgactgc tgggccgttt tcattcaacc ctgggggccc aaggcctaca tcacgtggat
     1441 cacgctcgcc gtctacattg tgcccgtcat cgtgcttgcc acctgctatg gccttatcag
     1501 cttcaagatc tggcagaatt tacggctcaa gacggcggga ggaaacgtct atatcaaggc
     1561 cgacaagcag aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg
     1621 cggcgtgcag ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1681 gctgcccgac aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa
     1741 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1801 cgagctgtac aagggcggta ccggagggag catggtgaga aagggcgagg agctgttcac
     1861 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1921 gtccggcgag ggtgagggcg atgccaccga gggcaagctg accctgaagt tcatctgcac
     1981 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2041 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2101 cgaaggctac atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2161 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2221 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacaccg gagcagcagc
     2281 aaagctcatc tctaaggcca agatccgcac ggtcaagatg accttcatcg tcgtgctggc
     2341 cttcatcgtg tgctggacgc cattcttttt cgtgcagatg tggagtgtct gggatgccga
     2401 tgcgcccaag gaagcctcac ctttcatcat cgccatgctc ctggccagcc tcaacagctg
     2461 ctgcaacccc tggatctaca tgctcttcac gggccacctc ttccaagaac ttgtgcagcg
     2521 cttcctctgc tgctcattcc gccgcctgaa aggcagccgg cctggggaga caagcgtcag
     2581 caaaaagagc aactcgtcta cctttgtcct gagccagtac agctccagcc agagaagatg
     2641 ctcgcagcca tccacgctgt gagctagcgc ccctctccct cccccccccc taacgttact
     2701 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     2761 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     2821 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     2881 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     2941 cggaaccccc cacctggcga caggtgcctc tgcggccaac gccacgtgta taagatacac
     3001 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3061 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3121 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     3181 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3241 aatatggcca caaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     3301 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3361 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3421 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     3481 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3541 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3601 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     3661 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     3721 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     3781 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     3841 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     3901 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     3961 aaggatggta aaaagaagaa aaagaagtca aagacaaagt gtgtaattat gtaagtcgac
     4021 gaacaaaaac tcatctcaga agaggatctg aatgctgtgg gccaggacac gcaggaggtc
     4081 atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat cctggccctg
     4141 gtggtgctca ccatcatctc ccttatcatc ctcatcatgc tttggcagaa gaagccacgt
     4201 taggcggccg ctcgagatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4441 tgggctctat ggcttctgag gcggaaagaa ccagtggcgg taatacggtt atccacagaa
     4501 tcgggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acgcgactta
     4921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg
     5341 gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg
     5401 tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta
     5461 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg
     5521 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     5581 ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg
     5641 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
     5701 aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt
     5761 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     5821 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     5881 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     5941 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     6001 aggcatcgcc ataggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     6061 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     6121 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     6181 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     6241 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     6301 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     6361 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
     6421 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     6481 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     6541 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     6601 gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     6661 tcagaggccg ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     6721 aatgggcgga actgggcgga gttaggggcg gaatgggcgg agttaggggc gggactatgg
     6781 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6841 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6901 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     6961 aggactcttc ctttttcaat aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7981 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     8041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     8461 ttacaattta c
//
