LOCUS       sequence-365774-        4197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A7_STOP
FEATURES             Location/Qualifiers
     source          1..4197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1754..1853)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1870..1889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1871..1889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1894..1910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1894..1910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2012..2031)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2023..2832
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2090..2109)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2979..3567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3468..3487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3721..3738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3897..3924)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4016..4102)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4151..4173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4165..4182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4166..4182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cttcgaggag
      121 ctgctggagc aagtgggagg atttggacca ttccagctga ggaacgtggc cctgctggcc
      181 ctgcctcggg tgctgctgcc actgcacttt ctgctgccca tcttcctggc cgccgtgcct
      241 gcacacaggt gcgcactgcc aggagcccct gccaacttta gccaccagga cgtgtggctg
      301 gaggcacacc tgccaagaga gccagatggc acactgagct cctgtctgag gttcgcctac
      361 ccccaggccc tgcctaatac cacactgggc gaggagagac agtccagggg agagctggag
      421 gacgagccag caaccgtgcc atgctcccag ggctgggagt atgatcactc tgagttctct
      481 agcacaatcg ccaccgagtc ccagtgggac ctggtgtgcg agcagaaggg cctgaataga
      541 gccgcctcta catttttttt cgctggggta ttggtgggag cagtggcctt tggctacctg
      601 agcgataggt tcggccggag aaggctgctg ctggtggcct atgtgagcac cctggtgctg
      661 ggactggcat ccgccgcctc tgtgagctac gtgatgtttg caatcacccg cacactgacc
      721 ggatccgccc tggcaggctt cacaatcatc gtgatgcccc tggagctgga gtggctggac
      781 gtggagcaca ggaccgtggc aggcgtgctg tcctctacat tttggaccgg aggcgtgatg
      841 ctgctggccc tggtgggcta tctgatcagg gattggaggt ggctgctgct ggcagtgaca
      901 ctgccttgcg caccaggaat cctgagcctg tggtgggtgc ctgagtccgc caggtggctg
      961 ctgacccagg gacacgtgaa ggaggcacac aggtacctgc tgcactgcgc aagactgaac
     1021 ggccggcccg tgtgcgagga ctccttttct caggaggccg tgtccaaggt ggcagcagga
     1081 gagagggtgg tgcgccggcc ttcttatctg gatctgttcc ggacaccacg cctgcggcac
     1141 atcagcctgt gctgcgtggt ggtgtggttt ggcgtgaact tctcctacta tggactgtct
     1201 ctggacgtga gcggactggg cctgaacgtg taccagaccc agctgctgtt tggcgccgtg
     1261 gagctgccct ctaagctgct ggtgtacctg agcgtgcgct atgcaggcag aaggctgaca
     1321 caggcaggca ccctgctggg aacagccctg gccttcggca caaggctgct ggtgagctcc
     1381 gatatgaaga gctggtccac cgtgctggcc gtgatgggca aggccttttc tgaggccgcc
     1441 ttcaccacag cctacctgtt taccagcgag ctgtatccta ccgtgctgag acagacaggc
     1501 atgggactga ccgccctggt gggcaggctg ggcggctctc tggccccact ggccgccctg
     1561 ctggacggcg tgtggctgag cctgcccaag ctgacctacg gaggaatcgc cctgctggca
     1621 gcaggaacag ccctgctgct gccagagacc agacaggccc agctgcccga gacaatccag
     1681 gatgtggaga ggaagtccgc ccccacctct ctgcaggagg aggagatgcc tatgaagcag
     1741 gtgcagaatt aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1801 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1861 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1921 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1981 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     2041 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     2101 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     2161 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2221 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     2281 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     2341 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2401 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2461 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2521 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2581 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2641 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2701 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2761 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2821 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2881 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3121 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3661 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3721 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3781 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3841 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3901 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3961 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     4021 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     4081 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     4141 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
