LOCUS       sequence-365816-        7983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expresseion of recombinant FAK using baccolovirus in SF9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Case LB, De Pasquale M, Henry L, Rosen MK
  TITLE     Synergistic phase separation of two pathways promotes integrin 
            clustering and nascent adhesion formation.
  JOURNAL   Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588.
  PUBMED    35049497
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-20"; volume: "11. pii: 72588. doi"; pages: " 
            10.7554/eLife.72588"
COMMENT     Sequence Label: FAK
FEATURES             Location/Qualifiers
     source          1..7983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(513..518,519..535,536..541))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        893..984
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     909..926
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1051..1068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1090..1110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    4462..4596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4499..4518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4553..4572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4625..4790
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4974..5428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5061..5080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5270..5291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5454..5558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5559..5627,5628..6419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5777..5796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6590..7178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7079..7098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7332..7349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7483..7707
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(7774..7983,1..324))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
       61 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
      121 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
      181 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
      241 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
      301 aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
      361 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
      421 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
      481 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
      541 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
      601 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
      661 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
      721 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
      781 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
      841 actctagcta tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga
      901 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
      961 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc
     1021 ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacga ttacgatatc
     1081 ccaacgaccg aaaacctgta ttttcagggc gccatgggga tccggatggc agctgcttat
     1141 cttgacccaa acttgaatca cacaccaagt tcgagtacta agactcacct gggtactggc
     1201 atggaacggt cccctggtgc aatggaacga gtattaaagg tctttcatca ttttgaaagc
     1261 agtagtgagc caaccacctg ggccagtatt atcaggcatg gagatgctac tgatgtcagg
     1321 ggcatcattc agaagatagt ggacagtcac aaagtaaagc acgtggcctg ctatggattt
     1381 cgcctcagtc acctgcgatc ggaggaggtg cactggctgc acgtggacat gggtgtctcc
     1441 agtgtgaggg agaaatatga actcgcccac ccaccagagg agtggaaata tgagttgaga
     1501 attcgttact tgccaaaagg atttctaaac cagtttactg aagataagcc aacattgaat
     1561 ttcttctatc aacaggtgaa gagtgactac atgcaagaaa tagctgatca agtagaccaa
     1621 gaaatagctt tgaagttggg ttgtttggaa attaggcgat cctattggga gatgaggggt
     1681 aatgccctag agaagaagtc caactatgaa gtattagaaa aagatgttgg tttaaagcga
     1741 ttttttccta agagtttact ggattctgtc aaggccaaaa cactaagaaa gctgatccag
     1801 caaacattca ggcagtttgc caaccttaat agagaagaaa gcatcctgaa attctttgag
     1861 attctttctc ctgtgtacag atttgacaaa gagtgcttca agtgtgccct tgggtcaagt
     1921 tggatcattt ctgtggaatt ggcaatcggc ccagaagaag ggatcagtta cctgacagac
     1981 aaaggctgca atcccacaca cctagcagac tttaaccaag tgcagaccat ccagtactca
     2041 aacagtgaag acaaagacag gaaaggaatg ctacaactca aaatcgctgg tgctcctgag
     2101 cctctgaccg tgacggcacc atccctaacc attgccgaga acatggccga cctgattgat
     2161 ggatactgcc ggctggtgaa tggagccaca caatctttca tcatcagacc ccagaaagaa
     2221 ggtgaacggg cgttgccatc aataccaaag ttggccaaca gtgaaaagca aggcatgcgg
     2281 acacatgcag tctctgtgtc agagacagat gactatgcag agatcatcga tgaggaagac
     2341 acatacacca tgccctcgac cagggattat gagattcaga gagaaagaat agaacttgga
     2401 cgctgtattg gagaaggtca gtttggagat gtacatcaag gcgtgtacct gagcccagag
     2461 aatccagctt tggctgttgc aatcaaaaca tgtaaaaact gtacttcgga cagcgtgaga
     2521 gagaagttcc ttcaagaagc cttaacaatg cgtcagtttg accatcctca catcgtgaag
     2581 ctgattggag tcattacaga gaaccctgtc tggataatca tggagttgtg cacacttgga
     2641 gagctgaggt catttttgca agtaagaaaa tacagcttgg acctggcatc tttgatatta
     2701 tatgcctatc agcttagtac agcactcgcg tatctggaga gcaaaagatt tgttcacagg
     2761 gacattgctg ctcggaatgt tctggtgtct tcaaatgatt gtgtaaaatt gggagacttt
     2821 ggattatctc gatatatgga agacagtact tactataaag cttccaaagg aaaattacct
     2881 attaaatgga tggctccaga gtcaatcaat tttcgacgtt ttacctcagc tagtgacgtg
     2941 tggatgtttg gtgtgtgtat gtgggagata ctgatgcatg gcgtgaagcc ttttcaagga
     3001 gtgaagaaca atgatgtgat cggtcgaatt gaaaatgggg aaagattacc aatgcctcca
     3061 aattgtcctc ccaccctcta cagccttatg acgaaatgtt gggcctatga ccccagcagg
     3121 cggcccaggt ttactgaact aaaagctcag ctcagcacaa tcctggagga ggagaaggtg
     3181 cagcaagaag aacggatgag gatggaatcc agaagacagg ctaccgtgtc ctgggactct
     3241 ggagggtctg atgaagcacc acccaagccc agcagacctg gttatcctag cccgagatcc
     3301 agtgaaggat tttatcccag cccacagcac atggtccaga ccaatcacta ccaggtctct
     3361 ggctacccgg gttcccatgg aatcccagcc atggctggca gcatttacca aggtcaggca
     3421 tctcttctgg accagacaga attgtggaat cacagacctc aggagatgtc catgtggcag
     3481 cccagtgtgg aggattcggc agctttggac cttcgaggga tgggacaggt gcttccccct
     3541 cacctgatgg aagagcggct aatccgacag cagcaggaaa tggaagaaga ccagcgctgg
     3601 ctggagaagg aagagcgatt cctgaaacct gatgtgaggc tctctcgagg cagcatcgac
     3661 agggaagacg ggagttttca gggtccgact ggaaaccaac acatctatca gcctgtgggg
     3721 aagccagatc ctgcagctcc accaaagaaa cctcctcgcc ctggagcacc tggccaccta
     3781 agcaacctgt ccagcatcag cagccctgca gacagctaca atgagggtgt caagcttcag
     3841 ccccaggaaa tcagcccccc tcccactgcc aaccttgacc ggtccaatga caaggtatat
     3901 gaaaatgtga caggcctagt gaaggctgtc atcgagatgt ccagcaaaat ccagccagct
     3961 cctccagaag agtacgtccc tatggtgaag gaagttggcc tggccctgcg gaccttacta
     4021 gccacggtgg atgagaccat tcctgctctt ccagccagca ctcatcgaga gatcgagatg
     4081 gcacagaagc tgctgaactc cgacttaggc gagctcatca gcaagatgaa gctggcgcag
     4141 cagtacgtca tgaccagcct gcagcaggag tataagaagc agatgctgac agctgctcac
     4201 gccctggctg tggatgccaa gaacctactt gatgttattg atcaagcaag actgaaaatg
     4261 ctagggcaga cacggccaca ctgagcggcc gctttcgaat ctagagcctg cagtctcgag
     4321 gcatgcggta ccaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     4381 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4441 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     4501 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4561 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgatatc gcctaggaga
     4621 tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
     4681 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
     4741 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt
     4801 tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc
     4861 ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat
     4921 gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc
     4981 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5041 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5101 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5161 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5221 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg
     5281 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5341 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5401 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5461 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5521 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5581 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5641 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5701 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5761 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5821 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5881 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5941 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6001 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6061 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6121 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6181 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6241 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6301 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6361 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6421 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6481 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7201 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7261 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7321 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7381 tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat
     7441 cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag
     7501 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     7561 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     7621 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     7681 gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc
     7741 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
     7801 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
     7861 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
     7921 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
     7981 ata
//
