LOCUS       sequence-365821-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expresseion of recombinant FAK using baccolovirus in SF9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Case LB, De Pasquale M, Henry L, Rosen MK
  TITLE     Synergistic phase separation of two pathways promotes integrin 
            clustering and nascent adhesion formation.
  JOURNAL   Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588.
  PUBMED    35049497
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-20"; volume: "11. pii: 72588. doi"; pages: " 
            10.7554/eLife.72588"
COMMENT     Sequence Label: FAK Pyk2
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  894..1118
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1185..1718)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1907..1912,1913..1929,1930..1935))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2287..2378
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2303..2320
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2445..2462
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2484..2504
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5721..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5758..5777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5812..5831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5884..6049
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6233..6687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6320..6339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6529..6550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6713..6817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6818..6886,6887..7678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7036..7055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     1321 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     1681 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1741 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
     1801 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     1861 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     1921 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     1981 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     2041 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     2101 gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
     2161 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     2221 tttgttcgcc caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat
     2281 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     2341 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     2401 ccaccatcgg gcgcggatct cggtccgaaa ccatgtcgta ctaccatcac catcaccatc
     2461 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg gggatccgga
     2521 tggcagctgc ttatcttgac ccaaacttga atcacacacc aagttcgagt actaagactc
     2581 acctgggtac tggcatggaa cggtcccctg gtgcaatgga acgagtatta aaggtctttc
     2641 atcattttga aagcagtagt gagccaacca cctgggccag tattatcagg catggagatg
     2701 ctactgatgt caggggcatc attcagaaga tagtggacag tcacaaagta aagcacgtgg
     2761 cctgctatgg atttcgcctc agtcacctgc gatcggagga ggtgcactgg ctgcacgtgg
     2821 acatgggtgt ctccagtgtg agggagaaat atgaactcgc ccacccacca gaggagtgga
     2881 aatatgagtt gagaattcgt tacttgccaa aaggatttct aaaccagttt actgaagata
     2941 agccaacatt gaatttcttc tatcaacagg tgaagagtga ctacatgcaa gaaatagctg
     3001 atcaagtaga ccaagaaata gctttgaagt tgggttgttt ggaaattagg cgatcctatt
     3061 gggagatgag gggtaatgcc ctagagaaga agtccaacta tgaagtatta gaaaaagatg
     3121 ttggtttaaa gcgatttttt cctaagagtt tactggattc tgtcaaggcc aaaacactaa
     3181 gaaagctgat ccagcaaaca ttcaggcagt ttgccaacct taatagagaa gaaagcatcc
     3241 tgaaattctt tgagattctt tctcctgtgt acagatttga caaagagtgc ttcaagtgtg
     3301 cccttgggtc aagttggatc atttctgtgg aattggcaat cggcccagaa gaagggatca
     3361 gttacctgac agacaaaggc tgcaatccca cacacctagc agactttaac caagtgcaga
     3421 ccatccagta ctcaaacagt gaagacaaag acaggaaagg aatgctacaa ctcaaaatcg
     3481 ctggtgctcc tgagcctctg accgtgacgg caccatccct aaccattgcc gagaacatgg
     3541 ccgacctgat tgatggatac tgccggctgg tgaatggagc cacacaatct ttcatcatca
     3601 gaccccagaa agaaggtgaa cgggcgttgc catcaatacc aaagttggcc aacagtgaaa
     3661 agcaaggcat gcggacacat gcagtctctg tgtcagagac agatgactat gcagagatca
     3721 tcgatgagga agacacatac accatgccct cgaccaggga ttatgagatt cagagagaaa
     3781 gaatagaact tggacgctgt attggagaag gtcagtttgg agatgtacat caaggcgtgt
     3841 acctgagccc agagaatcca gctttggctg ttgcaatcaa aacatgtaaa aactgtactt
     3901 cggacagtgt gagagagaag ttccttcaag aagccttaac aatgcgtcag tttgaccatc
     3961 ctcacatcgt gaagctgatt ggagtcatta cagagaaccc tgtctggata atcatggagt
     4021 tgtgcacact tggagagctg aggtcatttt tgcaagtaag aaaatacagc ttggacctgg
     4081 catctttgat attatatgcc tatcagctta gtacagcact cgcgtatctg gagagcaaaa
     4141 gatttgttca cagggacatt gctgctcgga atgttctggt gtcttcaaat gattgtgtaa
     4201 aattgggaga ctttggatta tctcgatata tggaagacag tacttactat aaagcttcca
     4261 aaggaaaatt acctattaaa tggatggctc cagagtcaat caattttcga cgttttacct
     4321 cagctagtga cgtgtggatg tttggtgtgt gtatgtggga gatactgatg catggcgtga
     4381 agccttttca aggagtgaag aacaatgatg tgatcggtcg aattgaaaat ggggaaagat
     4441 taccaatgcc tccaaattgt cctcccaccc tctacagcct tatgacgaaa tgttgggcct
     4501 atgaccccag caggcggccc aggtttactg aactaaaagc tcagctcagc acaatcctgg
     4561 aggaggagaa ggtgcagcaa gaagagcagg aacggaacgc tagatatcga actccaaaaa
     4621 ttcttgagcc cactgcgttc caagagcctc cgcctaaacc ttccagaccg aaatatagac
     4681 ctcctccgca gacgaatttg ctggctccta agttgcaatt tcaggttccc gagggtttgt
     4741 gtgctagttc acctacattg acgagtccga tggagtatcc ttctcccgta aattccttgc
     4801 acacgccacc gctgcaccgc cacaacgttt tcaaaagaca ctctatgcgg gaggaagatt
     4861 tcatacagcc ttcttcccgc gaggaggcgc aacagctttg ggaggcggaa aaagttaaga
     4921 tgcggcaaat ccttgacaaa caacagaagc aaatggtgga agattatcag tggctccgcc
     4981 aagaggagaa gagtcttgat cccatggttt acatgaacga taagagtccg ctgacacctg
     5041 agaaagaggt aggctacctg gagtttacgg gaccacctca aaagcctcct aggctcggcg
     5101 ctcaatcaat tcccacggcc aatctcgatc ggtccaatga caaggtttac gagaacgtaa
     5161 cgggattggt gaaggcagtc attgagatgt cttctaaaat ccaacctgcc ccgccggagg
     5221 aatatgtacc gatggtaaaa gaggttgggc tcgccctgcg gacactgctc gcaacggtcg
     5281 atgaaaccat acctctgttg ccagctagta ctcacaggga gatagagatg gcacaaaaac
     5341 tgctgaactc agacctggga gagcttataa acaaaatgaa gctcgcccag cagtatgtga
     5401 tgacgagctt gcagcaggaa tacaaaaagc aaatgttgac agctgcacat gcactcgccg
     5461 ttgacgcaaa aaatcttctg gatgttatag accaggcccg actcaagatg ctcgggcaga
     5521 caaggccaca ttgataatga taggcggccg ctttcgaatc tagagcctgc agtctcgagg
     5581 catgcggtac caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     5641 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5701 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5761 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5821 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgatatcg cctaggagat
     5881 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     5941 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     6001 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
     6061 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
     6121 tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg
     6181 tttgtaattg actgaatatc aacgcttatt tgcagcctga atggcgaatg ggacgcgccc
     6241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tcttaatagt ggactcttgt
     6541 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6661 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     6721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7501 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7561 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
