LOCUS       sequence-365830-        7953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of recombinant FAK using baculovirus in SF9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Case LB, De Pasquale M, Henry L, Rosen MK
  TITLE     Synergistic phase separation of two pathways promotes integrin 
            clustering and nascent adhesion formation.
  JOURNAL   Elife. 2022 Jan 20;11. pii: 72588. doi: 10.7554/eLife.72588.
  PUBMED    35049497
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-20"; volume: "11. pii: 72588. doi"; pages: " 
            10.7554/eLife.72588"
COMMENT     Sequence Label: FAK Fus
FEATURES             Location/Qualifiers
     source          1..7953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  894..1118
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1185..1718)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1907..1912,1913..1929,1930..1935))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2287..2378
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2303..2320
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2445..2462
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2484..2504
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4964..4975
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    5826..5960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5863..5882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5917..5936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5989..6154
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6338..6792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6634..6655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6818..6922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6923..6991,6992..7783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7141..7160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     1321 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     1681 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1741 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
     1801 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     1861 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     1921 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     1981 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     2041 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     2101 gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
     2161 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     2221 tttgttcgcc caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat
     2281 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     2341 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     2401 ccaccatcgg gcgcggatct cggtccgaaa ccatgtcgta ctaccatcac catcaccatc
     2461 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg gggatccgga
     2521 tggcagctgc ttatcttgac ccaaacttga atcacacacc aagttcgagt actaagactc
     2581 acctgggtac tggcatggaa cggtcccctg gtgcaatgga acgagtatta aaggtctttc
     2641 atcattttga aagcagtagt gagccaacca cctgggccag tattatcagg catggagatg
     2701 ctactgatgt caggggcatc attcagaaga tagtggacag tcacaaagta aagcacgtgg
     2761 cctgctatgg atttcgcctc agtcacctgc gatcggagga ggtgcactgg ctgcacgtgg
     2821 acatgggtgt ctccagtgtg agggagaaat atgaactcgc ccacccacca gaggagtgga
     2881 aatatgagtt gagaattcgt tacttgccaa aaggatttct aaaccagttt actgaagata
     2941 agccaacatt gaatttcttc tatcaacagg tgaagagtga ctacatgcaa gaaatagctg
     3001 atcaagtaga ccaagaaata gctttgaagt tgggttgttt ggaaattagg cgatcctatt
     3061 gggagatgag gggtaatgcc ctagagaaga agtccaacta tgaagtatta gaaaaagatg
     3121 ttggtttaaa gcgatttttt cctaagagtt tactggattc tgtcaaggcc aaaacactaa
     3181 gaaagctgat ccagcaaaca ttcaggcagt ttgccaacct taatagagaa gaaagcatcc
     3241 tgaaattctt tgagattctt tctcctgtgt acagatttga caaagagtgc ttcaagtgtg
     3301 cccttgggtc aagttggatc atttctgtgg aattggcaat cggcccagaa gaagggatca
     3361 gttacctgac agacaaaggc tgcaatccca cacacctagc agactttaac caagtgcaga
     3421 ccatccagta ctcaaacagt gaagacaaag acaggaaagg aatgctacaa ctcaaaatcg
     3481 ctggtgctcc tgagcctctg accgtgacgg caccatccct aaccattgcc gagaacatgg
     3541 ccgacctgat tgatggatac tgccggctgg tgaatggagc cacacaatct ttcatcatca
     3601 gaccccagaa agaaggtgaa cgggcgttgc catcaatacc aaagttggcc aacagtgaaa
     3661 agcaaggcat gcggacacat gcagtctctg tgtcagagac agatgactat gcagagatca
     3721 tcgatgagga agacacatac accatgccct cgaccaggga ttatgagatt cagagagaaa
     3781 gaatagaact tggacgctgt attggagaag gtcagtttgg agatgtacat caaggcgtgt
     3841 acctgagccc agagaatcca gctttggctg ttgcaatcaa aacatgtaaa aactgtactt
     3901 cggacagcgt gagagagaag ttccttcaag aagccttaac aatgcgtcag tttgaccatc
     3961 ctcacatcgt gaagctgatt ggagtcatta cagagaaccc tgtctggata atcatggagt
     4021 tgtgcacact tggagagctg aggtcatttt tgcaagtaag aaaatacagc ttggacctgg
     4081 catctttgat attatatgcc tatcagctta gtacagcact cgcgtatctg gagagcaaaa
     4141 gatttgttca cagggacatt gctgctcgga atgttctggt gtcttcaaat gattgtgtaa
     4201 aattgggaga ctttggatta tctcgatata tggaagacag tacttactat aaagcttcca
     4261 aaggaaaatt acctattaaa tggatggctc cagagtcaat caattttcga cgttttacct
     4321 cagctagtga cgtgtggatg tttggtgtgt gtatgtggga gatactgatg catggcgtga
     4381 agccttttca aggagtgaag aacaatgatg tgatcggtcg aattgaaaat ggggaaagat
     4441 taccaatgcc tccaaattgt cctcccaccc tctacagcct tatgacgaaa tgttgggcct
     4501 atgaccccag caggcggccc aggtttactg aactaaaagc tcagctcagc acaatcctgg
     4561 aggaggagaa ggtgcagcaa gaagaagcct caaacgatta tacccaacaa gcaacccaaa
     4621 gctatggggc ctaccccacc cagcccgggc agggctattc ccagcagagc agtcagccct
     4681 acggacagca gagttacagt ggttatagcc agtccacgga cacttcaggc tatggccaga
     4741 gcagctattc ttcttatggc cagagccaga acacaggcta tggaactcag tcaactcccc
     4801 agggatatgg ctcgactggc ggctatggca gtagccagag ctcccaatcg tcttacgggc
     4861 agcagtcctc ctatcctggc tatggccagc agccagctcc cagcagcacc tcgggaagtt
     4921 acggtagcag ttctcagagc agcagctatg ggcagcccca gagtgggagc tacagccagc
     4981 agcctagcta tggtggacag cagcaaagct atggacagca gcaaagctat aatccccctc
     5041 agggctatgg acagcagaac cagtacaaca gcagcagtgg tggtggaggt ggaggtggag
     5101 gtggaggtaa ctatggccaa gatcaatcct ccatgagtag tggtggtggc agtggtggcg
     5161 gttatggcaa tcaagaccag agtggtggag gtggcagcgg tggctatgga cagcaggacc
     5221 gtggacccac tgccaacctt gaccggtcca atgacaaggt atatgaaaat gtgacaggcc
     5281 tagtgaaggc tgtcatcgag atgtccagca aaatccagcc agctcctcca gaagagtacg
     5341 tccctatggt gaaggaagtt ggcctggccc tgcggacctt actagccacg gtggatgaga
     5401 ccattcctgc tcttccagcc agcactcatc gagagatcga gatggcacag aagctgctga
     5461 actccgactt aggcgagctc atcagcaaga tgaagctggc gcagcagtac gtcatgacca
     5521 gcctgcagca ggagtataag aagcagatgc tgacagctgc tcacgccctg gctgtggatg
     5581 ccaagaacct acttgatgtt attgatcaag caagactgaa aatgctaggg cagacacggc
     5641 cacactgagc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
     5701 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     5761 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5821 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5941 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     6001 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     6061 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     6121 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     6181 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     6241 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     6301 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6601 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     6661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6781 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7141 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7441 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7921 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
