LOCUS       sequence-365840-        8923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monitoring of HMGB1 subcellular localization by fluorescence 
            videomicroscopy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Liu P, Zhao L, Loos F, Iribarren K, Lachkar S, Zhou H, 
            Gomes-da-Silva LC, Chen G, Bezu L, Boncompain G, Perez F, Zitvogel 
            L, Kepp O, Kroemer G
  TITLE     Identification of pharmacological agents that induce HMGB1 release.
  JOURNAL   Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.1038/s41598-017-14848-1.
  PUBMED    29097772
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-11-2"; volume: "7(1):14915. doi"; pages: " 
            10.1038/s41598-017-14848-1"
COMMENT     Sequence Label: RUSH reporter-HMGB1-SBP-GFP
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2967..3077
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(3087..3089,3090..3800)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3087..3089,3090..3800)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3129..3150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3390..3409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3737..3758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3877..4088
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4101..4369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4396..4995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4396..4415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4892..4912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5002..5590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5055..5075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5473..5484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5664..5897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5969..6090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6006..6025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6060..6079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6130..6265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6298..6314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6298..6314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6311..6333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6322..6338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6346..6352,6353..6370,6371..6376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6391..6412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6529..6546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6700..7288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7459..8250,8251..8319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8082..8101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8320..8424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8492..8510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8548..8570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8670..8689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8883..8905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8897..8914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8898..8914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcaccggtgg atccggcgcg ccatgggcaa aggagatcct aagaagccga
     2341 gaggcaaaat gtcatcatat gcattttttg tgcaaacttg tcgggaggag cataagaaga
     2401 agcacccaga tgcttcagtc aacttctcag agttttctaa gaagtgctca gagaggtgga
     2461 agaccatgtc tgctaaagag aaaggaaaat ttgaagatat ggcaaaagcg gacaaggccc
     2521 gttatgaaag agaaatgaaa acctatatcc ctcccaaagg ggagacaaaa aagaagttca
     2581 aggatcccaa tgcacccaag aggcctcctt cggccttctt cctcttctgc tctgagtatc
     2641 gcccaaaaat caaaggagaa catcctggcc tgtccattgg tgatgttgcg aagaaactgg
     2701 gagagatgtg gaataacact gctgcagatg acaagcagcc ttatgaaaag aaggctgcga
     2761 agctgaagga aaaatacgaa aaggatattg ctgcatatcg agctaaagga aagcctgatg
     2821 cagcaaaaaa gggagttgtc aaggctgaaa aaagcaagaa aaagaaggaa gaggaggaag
     2881 atgaggaaga tgaagaggat gaggaggagg aggaagatga agaagatgaa gatgaagaag
     2941 aagatgatga tgatgaaggg aattccgacg agaagaccac tggttggcga ggtggacacg
     3001 ttgttgaagg actggctggg gaacttgaac aacttcgtgc acgactggag catcacccac
     3061 aaggtcaacg tgaaccacct gcaggtgtga gcaagggcga ggagctgttc accggggtgg
     3121 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3181 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3241 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3301 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3361 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3421 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3481 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3541 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3601 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3661 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3721 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3781 gcatggacga gctgtacaag ggccggcctt aaggcctcga gggccaatta attaagcggc
     3841 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     3901 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     3961 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     4021 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     4081 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     4141 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     4201 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     4261 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     4321 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     4381 cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4441 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4501 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4921 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4981 aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     5041 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5101 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5161 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5221 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5281 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5341 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5401 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5461 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5521 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5581 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     5701 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5881 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     5941 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6121 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6181 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6241 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     6301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     8761 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8821 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8881 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
