LOCUS       sequence-365946-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, Retroviral vector that encodes a 3LA mutant of
            53BP1 that disrupts three RIF1-binding motifs and abolishes the 
            53BP1-dependent recruitment of RIF1 to DNA double-strand breaks..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Setiaputra D, Escribano-Diaz C, Reinert JK, Sadana P, Zong D, Callen
            E, Sifri C, Seebacher J, Nussenzweig A, Thoma NH, Durocher D
  TITLE     RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1.
  JOURNAL   Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 
            10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24.
  PUBMED    35216668
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.01.025"; journalName: "Mol Cell"; date: 
            "2022-04-7- 7"; volume: "82"; issue: "7"; pages: "1359-1371.e9"
COMMENT     Sequence Label: pMX-53BP1(1-1710) 3LA
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(259..363)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             393..986
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1049..1406
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1471..1887
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1817..1839
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1855..1871
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1897..2271
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2209..2225
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             7451..7468
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             7490..7516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7526..7549
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7571..7591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7576..7905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7756..7891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7915..8514
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7915..7934)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8411..8431
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8741..9210
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9340..9357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9511..10099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9591..9610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10263..10865,1..189,190..258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
       61 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      361 gcgcacattt ccccgaaaag tgccacctgc ataatgaaag accccacctg taggtttggc
      421 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
      481 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
      541 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
      601 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
      661 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
      721 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
      781 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
      841 cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
      901 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
      961 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     1021 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
     1081 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
     1141 tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg
     1201 ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc
     1261 gatcgttttg gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga
     1321 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga
     1381 agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1441 tttctgtatt tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg
     1501 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
     1561 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
     1621 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
     1681 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
     1741 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
     1801 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
     1861 ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt atatggggca
     1921 cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct
     1981 ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg
     2041 gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac
     2101 acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac
     2161 acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac
     2221 gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatccg
     2281 aattccacgc ggccgcacgc gtaccatgga ccctactgga agtcagttgg attcagattt
     2341 ctctcagcaa gatactcctt gcctgataat tgaagattct cagcctgaaa gccaggttct
     2401 agaggatgat tctggttctc acttcagtat gctatctcga caccttccta atctccagac
     2461 gcacaaagaa aatcctgtgt tggatgttgt gtccaatcct gaacaaacag ctggagaaga
     2521 acgaggagac ggtaatagtg ggttcaatga acatttgaaa gaaaacaagg ttgcagaccc
     2581 tgtggattct tctaacttgg acacatgtgg ttccatcagt caggtcattg agcagttacc
     2641 tcagccaaac aggacaagca gtgttctggg aatgtcagtg gaatctgctc ctgctgtgga
     2701 ggaagagaag ggagaagagt tggaacagaa ggagaaagag aaggaagaag atacttcagg
     2761 caatactaca cattcccttg gtgctgaaga tactgcctca tcacagttgg gttttggggt
     2821 tctggaagct tcccagagcc aggatgttga ggaaaatact gtgccatatg aagtggacaa
     2881 agagcagcta caatcagtaa ccaccaactc tggttatacc aggctgtctg atgtggatgc
     2941 taatactgca attaagcatg aagaacagtc caacgaagat atccccatag cagaacagtc
     3001 cagcaaggac atccctgtga cagcacagcc cagtaaggat gtacatgttg taaaagagca
     3061 aaatccacca cctgcaaggt cagaggacat gccttttagc cccaaagcat ctgttgctgc
     3121 tatggaagca aaagaacagt tgtctgcaca agaacttatg gaaagtggac tgcagattca
     3181 gaagtcacca gagcctgagg ttttgtcaac tcaggaagac ttgtttgacc agagcaataa
     3241 aacagtatct tctgatggtt gctctactcc ttcaagggag gaaggtgggt gttctttggc
     3301 ttccactcct gccaccactc tgcatctcct gcagctctct ggtcagaggt cccttgttca
     3361 ggacagtctt tccacgaatt cttcagatct tgttgctcct tctcctgatg ctttccgatc
     3421 tactcctttt atcgttccta gcagtcccac agagcaagaa gggagacaag ataagccaat
     3481 ggacacgtca gtgttatctg aagaaggagg agagcctttt cagaagaaac ttcaaagtgg
     3541 tgaaccagtg gagttagaaa acccccctct cctgcctgag tccactgtat caccacaagc
     3601 ctcaacacca atatctcaga gcacaccagt cttccctcct gggtcacttc ctatcccatc
     3661 ccagcctcag ttttctcatg acatttttat tccttcccca agtctggaag aacaatcaaa
     3721 tgatgggaag aaagatggag atatgcatag ttcatctttg acagttgagt gttctaaaac
     3781 ttcagagatt gaaccaaaga attcccctga ggatcttggg ctatctttga caggggattc
     3841 ttgcaagttg atggcttcta caagtgaata tagtcagtcc ccaaagatgg agagcttgag
     3901 ttctcacaga attgatgaag atggagaaaa cacacagatt gaggatacgg aacccatgtc
     3961 tccagttctc aattctaaat ttgttcctgc tgaaaatgat agtatcctga tgaatccagc
     4021 acaggatggt gaagtacaac tgagtcagaa tgatgacaaa acaaagggag atgatacaga
     4081 caccagggat gacattagta ttttagccac tggttgcaag ggcagagaag aaacggtagc
     4141 agaagatgtt tgtattgatc tcacttgtga ttcggggagt caggcagttc cgtcaccagc
     4201 tactcgatct gaggcacttt ctagtgtgtt agatcaggag gaagctatgg aaattaaaga
     4261 acaccatcca gaggaggggt cttcagggtc tgaggtggaa gaaatccctg agacaccttg
     4321 tgaaagtcaa ggagaggaac tcaaagaaga aaatatggag agtgttccgt tgcacctttc
     4381 tgctactgaa actcagtccc aagggttgtg tcttcaaaag gaaatgccaa aaaaagaatg
     4441 ctcagaagct atggaagttg aaaccagtgt gattagtatt gattcccctc aaaagttggc
     4501 aatacttgac caagaattgg aacataagga acaggaagct tgggaagaag ctacttcaga
     4561 ggactccagt gttgtcattg tagatgtgaa agagccatct cccagagttg atgtttcttg
     4621 tgaacctttg gagggagtgg agaagtgctc agattcccag tcatgggagg atattgctcc
     4681 agaaatagaa ccatgtgctg agaatagatt agacaccaag gaagaaaaga gtgtagaata
     4741 tgaaggagat ctgaaatcag ggactgcaga aacagaacct gtagagcaag attcttcaca
     4801 gccttcctta cctttagtga gagcagatga tcctttaaga cttgaccagg agttgcagca
     4861 gccccaaact caggagaaaa caagtaattc attaacagaa gactcaaaaa tggctaatgc
     4921 aaagcagcta agctcagatg cagaggccca gaagctgggg aagccctctg cccatgcctc
     4981 acaaagcttc tgtgaaagtt ctagtgaaac cccatttcat ttcactttgc ctaaagaagg
     5041 tgatatcatc ccaccattga ctggtgcaac cccacctctt attgggcacc taaaattgga
     5101 gcccaagaga cacagtactc ctattggtat tagcaactat ccagaaagca ccatagcaac
     5161 cagtgatgtc atgtctgaaa gcatggtgga gacccatgat cccatacttg ggagtggaaa
     5221 aggggattct ggggctgccc cagacgtgga tgataaatta tgtctaagaa tgaaactggt
     5281 tagtcctgag actgaggcga gtgaagagtc tttgcagttc aacctggaaa agcctgcaac
     5341 tggtgaaaga aaaaatggat ctactgctgt tgctgagtct gttgccagtc cccaggagac
     5401 catgtctgtg ttgagctgta tctgtgaagc caggcaagag aatgaggctc gaagtgagga
     5461 tccccccacc acacccatca gggggaactt gctccacttt ccaagttctc aaggagaaga
     5521 ggagaaagaa aaattggagg gtgaccatac aatcaggcag agtcaacagc ctatgaagcc
     5581 cattagtcct gtcaaggacc ctgtttctcc tgcttcccag aagatggtca tacaagggcc
     5641 atccagtcct caaggagagg caatggtgac agatgtgcta gaagaccaga aagaaggacg
     5701 gagtactaat aaggaaaatc ctagtaaggc cttgattgaa aggcccagcc aaaataacat
     5761 aggaatccaa accatggagt gttccttgag ggtcccagaa actgtttcag cagcaaccca
     5821 gactataaag aatgtgtgtg agcaggggac cagtacagtg gaccagaact ttggaaagca
     5881 agatgccaca gttcagactg agagggggag tggtgagaaa ccagtcagtg ctcctgggga
     5941 tgatacagag tcgctccata gccagggaga agaagagttt gatatgcctc agcctccaca
     6001 tggccatgtc ttacatcgtc acatgagaac aatccgggaa gtacgcacac ttgtcactcg
     6061 tgtcattaca gatgtgtatt atgtggatgg aacagaagta gaaagaaaag taactgagga
     6121 gactgaagag ccaattgtag agtgtcagga gtgtgaaact gaagtttccc cttcacagac
     6181 tgggggctcc tcaggtgacc tgggggatat cagctccttc tcctccaagg catccagctt
     6241 acaccgcaca tcaagtggga caagtctctc agctatgcac agcagtggaa gctcagggaa
     6301 aggagccgga ccactcagag ggaaaaccag cgggacagaa cccgcagatt ttgccttacc
     6361 cagctcccga ggaggcccag gaaaactgag tcctagaaaa ggggtcagtc agacagggac
     6421 gccagtgtgt gaggaggatg gtgatgcagg ccttggcatc agacagggag ggaaggctcc
     6481 agtcacgcct cgtgggcgtg ggcgaagggg ccgcccacct tctcggacca ctggaaccag
     6541 agaaacagct gtgcctggcc ccttgggcat agaggacatt tcacctaact tgtcaccaga
     6601 tgataaatcc ttcagccgtg tcgtgccccg agtgccagac tccaccagac gaacagatgt
     6661 gggtgctggt gctttgcgtc gtagtgactc tccagaaatt cctttccagg ctgctgctgg
     6721 cccttctgat ggcttagatg cctcctctcc aggaaatagc tttgtagggc tccgtgttgt
     6781 agccaagtgg tcatccaatg gctactttta ctctgggaaa atcacacgag atgtcggagc
     6841 tgggaagtat aaattgctct ttgatgatgg gtacgaatgt gatgtgttgg gcaaagacat
     6901 tctgttatgt gaccccatcc cgctggacac tgaagtgacg gccctctcgg aggatgagta
     6961 tttcagtgca ggagtggtga aaggacatag gaaggagtct ggggaactgt actacagcat
     7021 tgaaaaagaa ggccaaagaa agtggtataa gcgaatggct gtcatcctgt ccttggagca
     7081 aggaaacaga ctgagagagc agtatgggct tggcccctat gaagcagtaa cacctcttac
     7141 aaaggcagca gatatcagct tagacaattt ggtggaaggg aagcggaaac ggcgcagtaa
     7201 cgtcagctcc ccagccaccc ctactgcctc cagtagcagc agcacaaccc ctacccgaaa
     7261 gatcacagaa agtcctcgtg cctccatggg agttctctca ggcaaaagaa aacttatcac
     7321 ttctgaagag gaacggtccc ctgccaagcg aggtcgcaag tctgccacag taaaacctgg
     7381 tgcagtaggg gcaggagagt ttgtgagccc ctgtgagagt ggagacaaca ccggtggagg
     7441 attcctgaac tgctgccccg gctgctgcat ggagcccagc ctcggaggat atccgtatga
     7501 tgttcctgat tatgctggag gaatggacta caaggacgac gatgacaagt aattaattaa
     7561 gtcgacctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc
     7921 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7981 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8041 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8101 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8161 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8221 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8281 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8341 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8401 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8461 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     8521 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gtcgacgata
     8581 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     8641 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     8701 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     8761 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     8821 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     8881 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     8941 tggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     9001 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     9061 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     9121 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     9181 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     9241 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     9301 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10201 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
    10261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10861 tgctc
//
