LOCUS       sequence-365947-       10895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, Retroviral vector that encodes 7A and 3LA 
            mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of 
            double-strand breaks and disrupts 53BP1 activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Setiaputra D, Escribano-Diaz C, Reinert JK, Sadana P, Zong D, Callen
            E, Sifri C, Seebacher J, Nussenzweig A, Thoma NH, Durocher D
  TITLE     RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1.
  JOURNAL   Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 
            10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24.
  PUBMED    35216668
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.01.025"; journalName: "Mol Cell"; date: 
            "2022-04-7- 7"; volume: "82"; issue: "7"; pages: "1359-1371.e9"
COMMENT     Sequence Label: pMX-53BP1(1-1710) 3LA7A
FEATURES             Location/Qualifiers
     source          1..10895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(117..221)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             251..844
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    907..1264
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1329..1745
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1675..1697
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1713..1729
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1755..2129
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2067..2083
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2155..2164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2167..2193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7339..7356
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             7378..7404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7414..7437
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7459..7479)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7464..7793
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7644..7779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7803..8402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7803..7822)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8299..8319
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8629..9098
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9228..9245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9399..9987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9479..9498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10151..10895,1..47,48..116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10774..10793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
       61 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
      181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
      241 ccacctgcat aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
      301 tttgcaaggc atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa
      361 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
      421 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
      481 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
      541 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
      601 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
      661 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact
      721 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
      781 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
      841 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
      901 ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga
      961 ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg
     1021 tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg
     1081 gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg
     1141 cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
     1201 cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct
     1261 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata
     1321 tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
     1381 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
     1441 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
     1501 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
     1561 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
     1621 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
     1681 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     1741 gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc
     1801 ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc
     1861 tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg
     1921 accgaccggt ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc
     1981 agactaagaa cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca
     2041 ccgccctcaa agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg
     2101 accccggggg tggaccatcc tctagactgc cggatccgaa ttccacgcgg ccgcgccacc
     2161 atggcctacc catacgatgt tcctgactat gcgatggacc ctactggaag tcagttggat
     2221 tcagatttct ctcagcaaga tactccttgc ctgataattg aagattctca gcctgaaagc
     2281 caggttctag aggatgattc tggttctcac ttcagtatgc tatctcgaca ccttcctaat
     2341 ctccagacgc acaaagaaaa tcctgtgttg gatgttgtgt ccaatcctga acaaacagct
     2401 ggagaagaac gaggagacgg taatagtggg ttcaatgaac atttgaaaga aaacaaggtt
     2461 gcagaccctg tggattcttc taacttggac acatgtggtt ccatcagtca ggtcattgag
     2521 cagttacctc agccaaacag gacaagcagt gttctgggaa tgtcagtgga atctgctcct
     2581 gctgtggagg aagagaaggg agaagagttg gaacagaagg agaaagagaa ggaagaagat
     2641 acttcaggca atactacaca ttcccttggt gctgaagata ctgcctcatc acagttgggt
     2701 tttggggttc tggaagcttc ccagagccag gatgttgagg aaaatactgt gccatatgaa
     2761 gtggacaaag agcagctaca atcagtaacc accaactctg gttataccag gctgtctgat
     2821 gtggatgcta atactgcaat taagcatgaa gaacagtcca acgaagatat ccccatagca
     2881 gaacagtcca gcaaggacat ccctgtgaca gcacagccca gtaaggatgt acatgttgta
     2941 aaagagcaaa atccaccacc tgcaaggtca gaggacatgc cttttagccc caaagcatct
     3001 gttgctgcta tggaagcaaa agaacagttg tctgcacaag aacttatgga aagtggactg
     3061 cagattcaga agtcaccaga gcctgaggtt ttgtcagctc aggaagactt gtttgaccag
     3121 agcaataaaa cagtatcttc tgatggttgc tctactcctt caagggagga aggtgggtgt
     3181 tctttggctt ccactcctgc caccactctg catctcctgc agctctctgg tcagaggtcc
     3241 cttgttcagg acagtctttc cacgaattct tcagatcttg ttgctccttc tcctgatgct
     3301 ttccgatcta ctccttttat cgttcctagc agtcccacag agcaagaagg gagacaagat
     3361 aagccaatgg acacgtcagt gttatctgaa gaaggaggag agccttttca gaagaaactt
     3421 caaagtggtg aaccagtgga gttagaaaac ccccctctcc tgcctgagtc cactgtatca
     3481 ccacaagcct caacaccaat atctcagagc acaccagtct tccctcctgg gtcacttcct
     3541 atcccagccc agcctcagtt ttctcatgac atttttattc cttccccaag tctggaagaa
     3601 caatcaaatg atgggaagaa agatggagat atgcatagtt catctttgac agttgagtgt
     3661 tctaaaactt cagagattga accaaagaat tcccctgagg atcttgggct atctttgaca
     3721 ggggattctt gcaagttgat ggcttctaca agtgaatatg ctcagtcccc aaagatggag
     3781 agcttgagtt ctcacagaat tgatgaagat ggagaaaacg cacagattga ggatacggaa
     3841 cccatgtctc cagttctcaa ttctaaattt gttcctgctg aaaatgatag tatcctgatg
     3901 aatccagcac aggatggtga agtacaactg agtcagaatg atgacaaaac aaagggagat
     3961 gatacagaca ccagggatga cattagtatt ttagccactg gttgcaaggg cagagaagaa
     4021 acggtagcag aagatgtttg tattgatctc acttgtgatt cgggggctca ggcagttccg
     4081 tcaccagcta ctcgatctga ggcactttct agtgtgttag atcaggagga agctatggaa
     4141 attaaagaac accatccaga ggaggggtct tcagggtctg aggtggaaga aatccctgag
     4201 acaccttgtg aaagtcaagg agaggaactc aaagaagaaa atatggagag tgttccgttg
     4261 cacctttctg ctactgaaac tcagtcccaa gggttgtgtc ttcaaaagga aatgccaaaa
     4321 aaagaatgct cagaagctat ggaagttgaa accagtgtga ttagtattga ttcccctcaa
     4381 aagttggcaa tacttgacca agaattggaa cataaggaac aggaagcttg ggaagaagct
     4441 acttcagagg actccagtgt tgtcattgta gatgtgaaag agccatctcc cagagttgat
     4501 gtttcttgtg aacctttgga gggagtggag aagtgctcag atgcccagtc atgggaggat
     4561 attgctccag aaatagaacc atgtgctgag aatagattag acaccaagga agaaaagagt
     4621 gtagaatatg aaggagatct gaaatcaggg actgcagaaa cagaacctgt agagcaagat
     4681 tcttcacagc cttccttacc tttagtgaga gcagatgatc ctttaagact tgaccaggag
     4741 ttgcagcagc cccaaactca ggagaaaaca agtaattcat taacagaaga ctcaaaaatg
     4801 gctaatgcaa agcagctaag ctcagatgca gaggcccaga agctggggaa gccctctgcc
     4861 catgccgcac aaagcttctg tgaaagttct agtgaaaccc catttcattt cactttgcct
     4921 aaagaaggtg atatcatccc accattgact ggtgcaaccc cacctcttat tgggcaccta
     4981 aaattggagc ccaagagaca cagtactcct attggtatta gcaactatcc agaaagcacc
     5041 atagcaacca gtgatgtcat gtctgaaagc atggtggaga cccatgatcc catacttggg
     5101 agtggaaaag gggattctgg ggctgcccca gacgtggatg ataaattatg tctaagaatg
     5161 aaactggtta gtcctgagac tgaggcgagt gaagagtctt tgcagttcaa cctggaaaag
     5221 cctgcaactg gtgaaagaaa aaatggatct actgctgttg ctgagtctgt tgccagtccc
     5281 cagaagacca tgtctgtgtt gagctgtatc tgtgaagcca ggcaagagaa tgaggctcga
     5341 agtgaggatc cccccaccac acccatcagg gggaacttgc tccactttcc aagttctcaa
     5401 ggagaagagg agaaagaaaa attggagggt gaccatacaa tcaggcagag tcaacagcct
     5461 atgaagccca ttagtcctgt caaggaccct gtttctcctg cttcccagaa gatggtcata
     5521 caagggccat ccagtcctca aggagaggca atggtgacag atgtgctaga agaccagaaa
     5581 gaaggacgga gtactaataa ggaaaatcct agtaaggcct tgattgaaag gcccagccaa
     5641 aataacatag gaatccaaac catggagtgt tccttgaggg tcccagaaac tgtttcagca
     5701 gcaacccaga ctataaagaa tgtgtgtgag caggggacca gtacagtgga ccagaacttt
     5761 ggaaagcaag atgccacagt tcagactgag agggggagtg gtgagaaacc agtcagtgct
     5821 cctggggatg atacagagtc gctccatagc cagggagaag aagagtttga tatgcctcag
     5881 cctccacatg gccatgtctt acatcgtcac atgagaacaa tccgggaagt acgcacactt
     5941 gtcactcgtg tcattacaga tgtgtattat gtggatggaa cagaagtaga aagaaaagta
     6001 actgaggaga ctgaagagcc aattgtagag tgtcaggagt gtgaaactga agtttcccct
     6061 tcacagactg ggggctcctc aggtgacctg ggggatatca gctccttctc ctccaaggca
     6121 tccagcttac accgcacatc aagtgggaca agtctctcag ctatgcacag cagtggaagc
     6181 tcagggaaag gagccggacc actcagaggg aaaaccagcg ggacagaacc cgcagatttt
     6241 gccttaccca gctcccgagg aggcccagga aaactgagtc ctagaaaagg ggtcagtcag
     6301 acagggacgc cagtgtgtga ggaggatggt gatgcaggcc ttggcatcag acagggaggg
     6361 aaggctccag tcacgcctcg tgggcgtggg cgaaggggcc gcccaccttc tcggaccact
     6421 ggaaccagag aaacagctgt gcctggcccc ttgggcatag aggacatttc acctaacttg
     6481 tcaccagatg ataaatcctt cagccgtgtc gtgccccgag tgccagactc caccagacga
     6541 acagatgtgg gtgctggtgc tttgcgtcgt agtgactctc cagaaattcc tttccaggct
     6601 gctgctggcc cttctgatgg cttagatgcc tcctctccag gaaatagctt tgtagggctc
     6661 cgtgttgtag ccaagtggtc atccaatggc tacttttact ctgggaaaat cacacgagat
     6721 gtcggagctg ggaagtataa attgctcttt gatgatgggt acgaatgtga tgtgttgggc
     6781 aaagacattc tgttatgtga ccccatcccg ctggacactg aagtgacggc cctctcggag
     6841 gatgagtatt tcagtgcagg agtggtgaaa ggacatagga aggagtctgg ggaactgtac
     6901 tacagcattg aaaaagaagg ccaaagaaag tggtataagc gaatggctgt catcctgtcc
     6961 ttggagcaag gaaacagact gagagagcag tatgggcttg gcccctatga agcagtaaca
     7021 cctcttacaa aggcagcaga tatcagctta gacaatttgg tggaagggaa gcggaaacgg
     7081 cgcagtaacg tcagctcccc agccacccct actgcctcca gtagcagcag cacaacccct
     7141 acccgaaaga tcacagaaag tcctcgtgcc tccatgggag ttctctcagg caaaagaaaa
     7201 cttatcactt ctgaagagga acggtcccct gccaagcgag gtcgcaagtc tgccacagta
     7261 aaacctggtg cagtaggggc aggagagttt gtgagcccct gtgagagtgg agacaacacc
     7321 ggtggaggat tcctgaactg ctgccccggc tgctgcatgg agcccagcct cggaggatat
     7381 ccgtatgatg ttcctgatta tgctggagga atggactaca aggacgacga tgacaagtaa
     7441 ttaattaagt cgacctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7501 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7561 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7621 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7681 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7741 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     7801 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     7861 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     7921 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     7981 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8041 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8101 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8161 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8221 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8281 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8341 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8401 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgt
     8461 cgacgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     8521 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
     8581 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
     8641 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
     8701 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     8761 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     8821 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     8881 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     8941 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     9001 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     9061 ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta
     9121 atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc
     9181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10081 ttcacctaga tccttttgcg gccggccgca aatcaatcta aagtatatat gagtaaactt
    10141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10261 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10861 tgttgagatc cagttcgatg taacccactc gtgca
//
