LOCUS       sequence-365953-        9266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible CRE activation in zebrafish transient transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Sinha DK, Neveu P, Gagey N, Aujard I, Le Saux T, Rampon C, Gauron C,
            Kawakami K, Leucht C, Bally-Cuif L, Volovitch M, Bensimon D, Jullien
            L, Vriz S
  TITLE     Photoactivation of the CreER T2 recombinase for conditional 
            site-specific recombination with high spatiotemporal resolution.
  JOURNAL   Zebrafish. 2010 Jun;7(2):199-204. doi: 10.1089/zeb.2009.0632.
  PUBMED    20441524
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/zeb.2009"; 
            journalName: "Zebrafish"; date: "2010-06"; volume: "7"; issue: "2"; 
            pages: "199-204"
COMMENT     Sequence Label: pT2EFCRET(#324)
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2500..2511,2512..2674)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     intron          3563..4135
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4143..4162
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4190..4208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4221..5249
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             5262..6197
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5310..6203
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    6223..6357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6247..6266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6301..6320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6796..6983,6984..6995)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7029..7045)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7029..7045)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7082..7102)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7082..7100)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7121..7137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7121..7137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7134..7156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7145..7161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7169..7175,7176..7193,7194..7199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7214..7235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7352..7369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7523..8111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7603..7622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8282..9073,9074..9142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8905..8924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9143..9247)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggggata caacaacaca cttaagtcac atttgcctac
     1621 gtaaagaaaa gtaaagaaaa tcaatagcta tattttacat ctcctttttt tgctgtcttt
     1681 taattagcct tgttttgctg ttatcttgat tgaaactgta acttcttcac ctgcttcttt
     1741 tctttgtagg ttttgccagc ccagaaacag gcatggctgt gcaaggccag agcaccatgc
     1801 acaatgcgct gcatgtcttc atgaacggct caatgtcctc agtccagggc tcagccaacg
     1861 accccatctt cctccttcac catgctttca ttgacaggta acaaacacgt catgacatta
     1921 gactgcacag tttttgacaa agttcataca atctgttgtt tatagctgct acaattagtg
     1981 aagtttgtga atgtacttgg atgagcagcg aaagatcaat tgagatcaat tgttagagtt
     2041 tggttgccct gcagagcaaa gaacaaaaaa taatctggtg gctttactgc gtgaggttat
     2101 tattggtgga atagaaacac aaaacataat tgcatttatt tgtttaattt tttatcttat
     2161 cttaactttc atcttgcata tttgtttctt acatcatttc tagcatcttt gagcgctggc
     2221 taagaactca tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca
     2281 atgacggcta ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt
     2341 ccaacaatgc tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac
     2401 cagaaatgcc ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc
     2461 tgttccacac aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca
     2521 aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa
     2581 aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac
     2641 ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc
     2701 ttttaatctc aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg
     2761 tttgtattta attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa
     2821 gttgtaaaac ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag
     2881 cctagctctt ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc
     2941 cgatgcggga agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag
     3001 cagagactcc ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg gggatcatct
     3061 aatcaagcac aaataagggg cgtgtaacac aaaagccagc gaccctttcc aatgcaaatc
     3121 aaacttgcaa ttctttgccg tttttatcat ttaagtgtcg gcttaaggtc cactatcaga
     3181 tgtaaacagc cttatctaac aaaggtatca ttacattctg aaattctcag gcatgcaagc
     3241 tagcttatga cgcactaggg agtgccaccc ttcctttcgc cctaacttcg tgataactcg
     3301 cgcgtttcac tcaacagctg catccgccct agtgctactg ggagttgtag tatacaagac
     3361 gcttacaggc tgaatgttct gtcaagaccc cgcctctagc actttgggaa ttctggactt
     3421 gatgatgtca tggttaatcc ccgcccagta gaggcggcta tataaagggt ggttaaggcc
     3481 cggttcgctc tcttcctcac cgggtctgcg gcgagttcta gctgaagctt cctgcaggtc
     3541 gaccgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
     3601 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
     3661 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
     3721 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     3781 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     3841 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     3901 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     3961 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     4021 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     4081 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     4141 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt aatacgactc
     4201 actatagggc gaattccacc atgtccaatt tactgaccgt acaccaaaat ttgcctgcat
     4261 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
     4321 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
     4381 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
     4441 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
     4501 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt
     4561 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
     4621 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc
     4681 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta
     4741 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
     4801 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
     4861 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
     4921 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
     4981 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
     5041 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
     5101 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
     5161 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
     5221 caatggtgcg cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg
     5281 ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt
     5341 ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt
     5401 ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc
     5461 tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg
     5521 atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga
     5581 ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc
     5641 tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc
     5701 tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca
     5761 aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc
     5821 tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc
     5881 tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc
     5941 tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga
     6001 agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc
     6061 tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg
     6121 ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag
     6181 agggtttccc tgccacagct tgatgaagat ctgagcgatg atgatccaga catgataaga
     6241 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     6301 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6361 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6421 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc
     6481 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     6541 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     6601 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     6661 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     6721 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     6781 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     6841 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     6901 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     6961 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     7021 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     7081 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7321 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7381 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7441 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7501 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7561 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7621 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7681 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7741 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7801 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7861 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7921 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7981 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8041 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8101 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8161 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8221 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9241 ttccgcgcac atttccccga aaagtg
//
