LOCUS       sequence-365973-        2891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in 
            mammalian cells with localization in the endoplasmic reticulum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2891)
  TITLE     Organelle-specific, mTurquoise2-HaloTag fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HT7-mTurquoise2-KDEL
FEATURES             Location/Qualifiers
     source          1..2891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     63..116
                     /codon_start=1
                     /product="signal sequence of chicken lysozyme"
                     /label=lysozyme signal sequence
                     /translation="MRSLLILVLCFLPLAALG"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..1058
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             join(1059..1061,1062..1772)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1101..1122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1362..1381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1709..1730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1906..2027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1997..2016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2034..2489)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2171..2192)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2383..2402
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2516..2620
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2622..2813
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2645..2665)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcca
       61 ccatgaggtc tttgctaatc ttggtgcttt gcttcctgcc cctggctgct ctggggaaag
      121 tctttggacg atgtgagctg gcagcggcta tgaagccacc ggtcgccacc atggaaatcg
      181 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
      241 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
      301 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
      361 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
      421 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
      481 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
      541 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
      601 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
      661 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
      721 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
      781 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
      841 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
      901 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
      961 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     1021 agatcgcgcg ctggctgtcc acgctcgaga tttccggcgt gagcaagggc gaggagctgt
     1081 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1141 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1201 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg
     1261 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1321 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1381 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1441 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg
     1501 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     1561 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1621 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     1681 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1741 ggatcactct cggcatggac gagctgtaca aggatgaatt gtaagcggcc gcgactctag
     1801 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1861 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     2041 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2101 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2161 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2221 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2281 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2341 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2401 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2461 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     2521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2581 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     2641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2701 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2881 ctaatttttt t
//
