LOCUS       sequence-365980-        2145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B-gTEMP for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Lu K, Wazawa T, Sakamoto J, Vu CQ, Nakano M, Kamei Y, Nagai T
  TITLE     Intracellular Heat Transfer and Thermal Property Revealed by 
            Kilohertz Temperature Imaging with a Genetically Encoded 
            Nanothermometer.
  JOURNAL   Nano Lett. 2022 Jul 27;22(14):5698-5707. doi: 
            10.1021/acs.nanolett.2c00608. Epub 2022 Jul 6.
  PUBMED    35792763
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.2c00608"; journalName: "Nano Lett"; date: 
            "2022-07-27- 27"; volume: "22"; issue: "14"; pages: "5698-5707"
COMMENT     Sequence Label: B-gTEMP/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             715..2145
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atggtgtcca agggcgaaga ggacaacatg gccagcctgc ctgccaccca cgagctgcac
       61 atcttcggca gcatcaacgg cgtggacttc gacatggtgg gacagggcac cggcaacccc
      121 aacgacggct acgaggaact gaacctgaag tccacaaagg gcgacctgca gttcagcccc
      181 tggattctgg tgccccacat cggctacggc ttccaccagt acctgcccta ccccgacggc
      241 atgagccctt tccaggccgc tatggtggat ggcagcggct accaggtgca ccggaccatg
      301 cagtttgagg acggcgccag cctgaccgtg aactaccggt acacatacga gggcagccac
      361 atcaagggcg aggcccaagt gaagggcaca ggctttccag ccgacggccc cgtgatgacc
      421 aatagcctga cagccgccga ctggtgcaga agcaagaaaa cctaccccaa tgacaagacc
      481 atcatcagca ccttcaagtg gtcctacacc accggcaatg gcaagcggta cagaagcacc
      541 gcccggacca cctacacctt cgccaaacct atggccgcca actacctgaa gaaccagcct
      601 atgtacgtgt tccgcaagac cgagctgaag cactccaaga cagaactgaa cttcaaagag
      661 tggcagaaag ccttcaccga cgtgatgggc atggacgagc tgtacaaggg taccatggtg
      721 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      781 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      841 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      901 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
      961 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1021 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1081 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1141 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1201 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1261 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     1321 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1381 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     1441 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     1501 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1561 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1621 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     1681 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1741 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     1801 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1861 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     1921 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     1981 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     2041 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     2101 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaa
//
