LOCUS       sequence-365998-        1750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ectopic expression of wild-type GBA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Smith LJ, Bolsinger MM, Chau KY, Gegg ME, Schapira AHV
  TITLE     The GBA variant E326K is associated with alpha-synuclein aggregation
            and lipid droplet accumulation in human cell lines.
  JOURNAL   Hum Mol Genet. 2022 Sep 20:ddac233. doi: 10.1093/hmg/ddac233.
  PUBMED    36130205
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2022 Sep 20:ddac233. doi: 10.1093/hmg/ddac233."
COMMENT     Sequence Label: WT GBA pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..30
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11..29
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccgcttgaa taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
       61 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca
      121 cagtggcggc cgcgagggga tggagttttc aagtccttcc agagaggaat gtcccaagcc
      181 tttgagtagg gtaagcatca tggctggcag cctcacagga ttgcttctac ttcaggcagt
      241 gtcgtgggca tcaggtgccc gcccctgcat ccctaaaagc ttcggctaca gctcggtggt
      301 gtgtgtctgc aatgccacat actgtgactc ctttgacccc ccgacctttc ctgcccttgg
      361 taccttcagc cgctatgaga gtacacgcag tgggcgacgg atggagctga gtatggggcc
      421 catccaggct aatcacacgg gcacaggcct gctactgacc ctgcagccag aacagaagtt
      481 ccagaaagtg aagggatttg gaggggccat gacagatgct gctgctctca acatccttgc
      541 cctgtcaccc cctgcccaaa atttgctact taaatcgtac ttctctgaag aaggaatcgg
      601 atataacatc atccgggtac ccatggccag ctgtgacttc tccatccgca cctacaccta
      661 tgcagacacc cctgatgatt tccagttgca caacttcagc ctcccagagg aagataccaa
      721 gctcaagata cccctgattc accgagccct gcagttggcc cagcgtcccg tttcactcct
      781 tgccagcccc tggacatcac ccacttggct caagaccaat ggagcggtga atgggaaggg
      841 gtcactcaag ggacagcccg gagacatcta ccaccagacc tgggccagat actttgtgaa
      901 gttcctggat gcctatgctg agcacaagtt acagttctgg gcagtgacag ctgaaaatga
      961 gccttctgct gggctgttga gtggataccc cttccagtgc ctgggcttca cccctgaaca
     1021 tcagcgagac ttcattgccc gtgacctagg tcctaccctc gccaacagta ctcaccacaa
     1081 tgtccgccta ctcatgctgg atgaccaacg cttgctgctg ccccactggg caaaggtggt
     1141 actgacagac ccagaagcag ctaaatatgt tcatggcatt gctgtacatt ggtacctgga
     1201 ctttctggct ccagccaaag ccaccctagg ggagacacac cgcctgttcc ccaacaccat
     1261 gctctttgcc tcagaggcct gtgtgggctc caagttctgg gagcagagtg tgcggctagg
     1321 ctcctgggat cgagggatgc agtacagcca cagcatcatc acgaacctcc tgtaccatgt
     1381 ggtcggctgg accgactgga accttgccct gaaccccgaa ggaggaccca attgggtgcg
     1441 taactttgtc gacagtccca tcattgtaga catcaccaag gacacgtttt acaaacagcc
     1501 catgttctac caccttggcc acttcagcaa gttcattcct gagggctccc agagagtggg
     1561 gctggttgcc agtcagaaga acgacctgga cgcagtggca ctgatgcatc ccgatggctc
     1621 tgctgttgtg gtcgtgctaa accgctcctc taaggatgtg cctcttacca tcaaggatcc
     1681 tgctgtgggc ttcctggaga caatctcacc tggctactcc attcacacct acctgtggcg
     1741 tcgccagtga
//
