LOCUS       sequence-366026-        8528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent oxytocin(OT) sensor 
            GRAB_OT1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Qian T, Wang H, Wang P, Geng L, Mei L, Osakada T, Wang L, Tang Y, 
            Kania A, Grinevich V, Stoop R, Lin D, Luo M, Li Y
  TITLE     A genetically encoded sensor measures temporal oxytocin release from
            different neuronal compartments.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2.
  PUBMED    36593404
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2."
COMMENT     Sequence Label: pDisplay-GRAB_OT1.0
FEATURES             Location/Qualifiers
     source          1..8528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1786..1807
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1913..1934)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2174..2193)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2727..3300,3301..3303,3304..3312)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2894..2911)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3120..3139
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3313..4020
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3458..3476)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3726..3745
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4078..4107
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4111..4257
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4279..4296)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4285..4509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4641..5229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4721..4740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5558..5605
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5630..5649
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5837..6631)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5948..5967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6558..6577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6730..6749)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6828..7019
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6976..6996
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7079..7870,7871..7939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7702..7721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7940..8044)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8071..8526
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8158..8177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8368..8389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgagggtgc
      841 gtttgcggct aactggagcg ctgaggcggt caacgggagc gcggcgccgc cgggaaccga
      901 gggcaatcgc actgccgggc cgccacagcg caacgaggcc ctggcgcggg tggaggtggc
      961 cgtgctgtgc ctcatcctgt tcctggcgct gagcggcaac gcgtgcgtgc tgctagcgct
     1021 gcgcaccacg cgccacaagc actcgcgcct cttcttcttc atgaagcacc tgagcatagc
     1081 cgacctggta gtggcggtgt tccaggtgct gccgcagctt ctgtgggaca tcacgttccg
     1141 cttctacggg cccgacctgc tgtgccgcct cgtcaagtac ctgcaggttg tgggcatgtt
     1201 cgcgtccacc tacctgctgc tgctcatgtc gctcgaccgc tgcctggcca tctgccagcc
     1261 gctgcgctcg ctgagccgcc gcaccgaccg cctggcggta ctcgtcacat ggctcggctg
     1321 cctggtggcc agcgcgccgc aggtgcacat cttctcgctg cgcgaggtgg ccgacggtgt
     1381 cttcgactgc tgggccgttt tcattcaacc ctgggggccc aaggcctaca tcacgtggat
     1441 cacgctcgcc gtctacattg tgcccgtcat cgtgcttgcc acctgctatg gccttatcag
     1501 cttcaagatc tggcagaatt tacggctcaa gacggcgggg ccgcgcgaca ccgacgcgct
     1561 ggacctggag gagggaggaa acgtctatat caaggccgac aagcagaaga acggcatcaa
     1621 ggcgaacttc cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
     1681 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1741 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1801 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
     1861 agggagcatg gtgagaaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1921 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
     1981 caccgagggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2041 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2101 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
     2161 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2221 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2281 ggggcacaag ctggagtaca acaccggagc agcagcacgc tggcgcgggc ggcagaacaa
     2341 gctcatctct aaggccaaga tccgcacggt caagatgacc ttcatcgtcg tgctggcctt
     2401 catcgtgtgc tggacgccat tctttttcgt gcagatgtgg agtgtctggg atgccgatgc
     2461 gcccaaggaa gcctcacctt tcatcatcgc catgctcctg gccagcctca acagctgctg
     2521 caacccctgg atctacatgc tcttcacggg ccacctcttc caagaacttg tgcagcgctt
     2581 cctctgctgc tcattccgcc gcctgaaagg cagccggcct ggggagacaa gcgtcagcaa
     2641 aaagagcaac tcgtctacct ttgtcctgag ccagtacagc tccagccaga gaagatgctc
     2701 gcagccatcc acgctgtgag ctagcgcccc tctccctccc ccccccctaa cgttactggc
     2761 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2821 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2881 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2941 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3001 aaccccccac ctggcgacag gtgcctctgc ggccaacgcc acgtgtataa gatacacctg
     3061 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     3121 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     3181 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa
     3241 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     3301 atggccacaa ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3361 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3481 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3541 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3781 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3841 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3901 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4021 gatggtaaaa agaagaaaaa gaagtcaaag acaaagtgtg taattatgta agtcgacgaa
     4081 caaaaactca tctcagaaga ggatctgaat gctgtgggcc aggacacgca ggaggtcatc
     4141 gtggtgccac actccttgcc ctttaaggtg gtggtgatct cagccatcct ggccctggtg
     4201 gtgctcacca tcatctccct tatcatcctc atcatgcttt ggcagaagaa gccacgttag
     4261 gcggccgctc gagatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4321 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4381 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4441 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4501 gctctatggc ttctgaggcg gaaagaacca gtggcggtaa tacggttatc cacagaatcg
     4561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagacg cgacttatcg
     4981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5041 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     5401 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     5461 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     5521 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     5581 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     5641 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     5701 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     5761 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     5821 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     5881 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     5941 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     6001 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     6061 catcgccata ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     6121 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     6181 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     6241 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     6301 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     6361 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     6421 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     6481 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     6541 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     6601 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     6661 cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     6721 gaggccgggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     6781 gggcggaact gggcggagtt aggggcggaa tgggcggagt taggggcggg actatggttg
     6841 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     6901 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     6961 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg
     7021 actcttcctt tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8041 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     8101 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8161 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8221 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8281 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8341 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8401 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8461 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     8521 caatttac
//
