LOCUS       sequence-366058-        4958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pEvol-based plasmid containing one inducible (by arabinose) copy 
            of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found 
            in pEvol plasmids was deleted..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Israeli B, Strugach DS, Gelkop S, Weber S, Gozlan DS, Amiram M
  TITLE     Genetically Encoding Light-Responsive Protein-Polymers Using 
            Translation Machinery for the Multi-Site Incorporation of 
            Photo-Switchable Unnatural Amino Acids
  JOURNAL   Adv. Funct. Mater. 2021, 31, 2011276
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2021, 31, 2011276"
COMMENT     Sequence Label: AzoRS-4
FEATURES             Location/Qualifiers
     source          1..4958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(65..943)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        970..1254
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1177..1196
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2218..2235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2291..2308)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(3044..3703)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3618..3637
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3704..3806)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4198..4215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4332..4877)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4412..4431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccatacgat ataagttgta attctcatgt ttgacagctt atcatcgata agcttggtac
       61 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
      121 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
      181 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
      241 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
      301 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
      361 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
      421 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
      481 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
      541 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
      601 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
      661 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
      721 ggaacagcaa aatatcactc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
      781 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
      841 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
      901 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
      961 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     1021 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     1081 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     1141 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     1201 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
     1261 ttttttgggc taacaggagg aattagatct atggacgaat ttgaaatgat aaagagaaac
     1321 acatctgaaa ttatcagcga ggaagagtta agagaggttt taaaaaaaga tgaaaaatct
     1381 gctgggatag ggtttgaacc aagtggtaaa atacatttag ggcattatct ccaaataaaa
     1441 aagatgattg atttacaaaa tgctggattt gatataatta tagttttggc tgatttacac
     1501 gcctatttaa accagaaagg agagttggat gagattagaa aaataggaga ttataacaaa
     1561 aaagtttttg aagcaatggg gttaaaggca aaatatgttt atggaagtga attccagctt
     1621 gataaggatt atacactgaa tgtctataga ttggctttaa aaactacctt aaaaagagca
     1681 agaaggagta tggaacttat agcaagagag gatgaaaatc caaaggttgc tgaagttatc
     1741 tatccaataa tgcaggttaa tgggtatcat tataggggcg ttgatgttgc ggttggaggg
     1801 atggagcaga gaaaaataca catgttagca agggagcttt taccaaaaaa ggttgtttgt
     1861 attcacaacc ctgtcttaac gggtttggat ggagaaggaa agatgagttc ttcaaaaggg
     1921 aattttatag ctgttgatga ctctccagaa gagattaggg ctaagataaa gaaagcatac
     1981 tgcccagctg gagttgttga aggaaatcca ataatggaga tagctaaata cttccttgaa
     2041 tatcctttaa ccataaaagg gccagaaaaa tttggtggag atttgacagt taatagctat
     2101 gaggagttag agagtttatt taaaaataag gaattgcatc caatgcgctt aaaaaatgct
     2161 gtagctgaag aacttataaa gattttagag ccaattagaa agagattata agtcgaccat
     2221 catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc ggatgagaga
     2281 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     2341 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     2401 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     2461 caaataaaac gaaaggctca gtcgaaagac tgggccttgt ttgtgagctc ccggtcatca
     2521 atcatcccca taatccttgt tagattatca aattctgctt ttcttcgcga attaattccg
     2581 cttcgcaaca tgtgagcacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
     2641 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
     2701 tcagtgcacg gctaactaag cggcctgctg actttctcgc cgatcaaaag gcattttgct
     2761 attaagggat tgacgagggc gtatctgcgc agtaagatgc gccccgcatt ccggcggtag
     2821 ttcagcaggg cagaacggcg gactctaaat ccgcatggca ggggttcaaa tcccctccgc
     2881 cggaccaaat tcgaaaagcc tgctcaacga gcaggctttt ttgcatgctc gagcagctca
     2941 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     3001 agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     3061 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     3121 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     3181 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     3241 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     3301 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     3361 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     3421 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     3481 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     3541 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     3601 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     3661 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     3721 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     3781 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
     3841 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
     3901 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
     3961 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     4021 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     4081 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     4141 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     4201 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     4261 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     4321 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     4381 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     4441 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4501 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4561 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     4621 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     4681 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     4741 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     4801 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     4861 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     4921 tgcaatttat ctcttcaaat gtagcacctg aagtcagc
//
