LOCUS       sequence-366065-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human MOSPD2 (mutant W201E, unable to bind lipid 
            droplets) fused to EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Zouiouich M, Di Mattia T, Martinet A, Eichler J, Wendling C, 
            Tomishige N, Grandgirard E, Fuggetta N, Fromental-Ramain C, Mizzon 
            G, Dumesnil C, Carpentier M, Reina-San-Martin B, Mathelin C, Schwab 
            Y, Thiam AR, Kobayashi T, Drin G, Tomasetto C, Alpy F
  TITLE     MOSPD2 is an endoplasmic reticulum-lipid droplet tether functioning 
            in LD homeostasis.
  JOURNAL   J Cell Biol. 2022 Jun 6;221(6). pii: 213116. doi: 
            10.1083/jcb.202110044. Epub 2022 Apr 7.
  PUBMED    35389430
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-06-6"; volume: "221(6). pii: 213116. doi"; 
            pages: " 10.1083/jcb.202110044. Epub 2022 Apr 7"
COMMENT     Sequence Label: pQCXIP GFP-MOSPD2 W201E
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             509..684
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    747..946
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1147..1563
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1493..1515
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1531..1547
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1600..1903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1904..2107
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2104..2128
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2245..2247,2248..2250,2251..2961)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2245..2247,2248..2250,2251..2961)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2290..2311)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2551..2570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2898..2919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4582..5156,5157..5159,5160..5168)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4749..4766)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4976..4995
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5188..5787
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5188..5207)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5684..5704
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6161..6586
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6689..6711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6847..6867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6852..7181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7032..7167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7203..7222
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7303..7320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7474..8062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7554..7573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8233..9024,9025..9093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8856..8875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9094..9198)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9266..9284
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9384..9459,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctca ataaaagagc ccacaacccc tcactcggcg
      541 cgccagtctt ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc
      601 tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      661 tacccacgac gggggtcttt catttggggg ctcgtccggg atttggagac ccctgcccag
      721 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat
      781 tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt actagttagc taactagctc
      841 tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg
      901 agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg
      961 tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc
     1021 ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg
     1081 cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
     1141 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     1201 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     1261 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     1321 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     1381 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     1441 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     1501 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     1561 gccggaattg aagatctggg ggatcgatcc tctagagtcc gttacataac ttacggtaaa
     1621 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1681 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1741 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     1801 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     1861 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     1921 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     1981 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2041 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2101 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2161 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgga
     2221 cctgcaggaa ttgatccgcg gccgatggtg agcaagggcg aggagctgtt caccggggtg
     2281 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2341 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2401 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2461 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2521 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2581 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2641 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2701 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2761 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2821 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2881 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2941 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
     3001 gagaatcacg cccagaataa agccaagctc atctctgaga cccggaggag gttcgaagct
     3061 gagtatgtga cagataagtc agataaatat gatgcacgtg atgttgaaag gctacaacaa
     3121 gatgataact gggttgaaag ttacttatct tggagacata atattgtaga tgaaacactg
     3181 aagatgctcg atgagagttt tcagtggagg aaagaaattt ctgtcaatga ccttaatgaa
     3241 tcctccattc ccagatggtt attggaaatt ggtgttattt atctccatgg ttatgacaaa
     3301 gaaggtaaca aattgttctg gatcagggtg aagtatcatg taaaagacca gaaaaccata
     3361 ttggacaaaa agaagctcat agcattctgg ttggaacgtt atgctaagag ggaaaatggg
     3421 aaacctgtaa cagtgatgtt tgacctgtca gaaactggaa taaatagcat tgacatggac
     3481 tttgtacgct ttatcatcaa ctgctttaag gtttattacc ctaaatacct ctcaaaaata
     3541 gtgatctttg atatgccttg gttaatgaat gctgctttca aaattgtgaa aaccgaactt
     3601 ggtccagaag cagtgagctt gttgaagttt acaagcaaaa atgaagtcca ggactatgtc
     3661 agtgtagaat acctgcctcc ccacatgggt ggaactgatc ctttcaagta tagctatcca
     3721 ccactagtag atgatgactt ccagacccca ctgtgtgaga atgggcctat taccagtgag
     3781 gatgaaactt caagtaaaga agacatagaa agtgatggca aagaaacatt ggaaacaatt
     3841 tctaatgaag aacaaacacc tcttcttaaa aagattaacc caaccgaatc tacttccaaa
     3901 gcagaagaaa atgaaaaagt tgattcaaaa gtgaaagctt tcaagaaacc attgagtgta
     3961 tttaaaggcc ccttactaca catcagccca gcagaagaac tgtactttgg aagtacagaa
     4021 tccggagaga agaaaacctt aatagtgttg acaaatgtaa ctaaaaatat agtggcattt
     4081 aaggtgagaa caacagctcc agaaaaatac agagtcaagc caagcaatag cagctgtgac
     4141 ccgggtgcat cagtggatat agttgtgtct ccccatgggg gtttaacagt ctctgcccaa
     4201 gaccgttttc tgataatggc tgcagaaatg gaacagtcat ctggcacagg cccagcagaa
     4261 ttaactcagt tttggaaaga agttcccaga aacaaagtga tggaacatag gttaagatgc
     4321 catactgttg aaagcagtaa accaaacact cttacgttaa aagacaatgc tttcaatatg
     4381 tcagataaaa ccagtgaaga tatatgtcta caactcagtc gtttactaga aagcaatagg
     4441 aagcttgaag accaagttca gcgttgtatc tggttccagc agctgctgct ttccttaaca
     4501 atgctcttgc ttgcttttgt cacctctttc ttctatttat tgtacagtta aggatccgga
     4561 attccgcccc cccccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     4621 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4681 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4741 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4801 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4861 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4921 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4981 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     5041 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     5101 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
     5161 ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
     5221 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5281 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5341 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5401 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5461 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5521 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5581 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5641 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5701 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5761 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     5821 aggagcgcac gaccccatgg ctccgaccga agccacccgg ggcggccccg ccgaccccgc
     5881 acccgccccc gaggcccacc gactctagtc gagggctgca gcgctgcaga ggccgagtgc
     5941 agaactgctc caaagggacc tcaaggcttt ccgagggaca ctaggctgac tccatcgagc
     6001 cagtgtagag ataagcttat cgattagtcc aatttgttaa agacaggata tcagtggtcc
     6061 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     6121 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     6181 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     6241 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc
     6301 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     6361 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     6421 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     6481 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     6541 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     6601 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc
     6661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc
     6841 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6901 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6961 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7141 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta
     7201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7261 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     7321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8521 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     8581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8821 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     8881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     9241 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     9301 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     9361 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
     9421 tacggggtca ttagttcata gcccatatat ggagttccg
//
