LOCUS       sequence-366111-        8407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of mouse cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTRE3G-mouse cGASdeltaN-GFP
FEATURES             Location/Qualifiers
     source          1..8407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        334..537
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             538..713
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    776..975
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1176..1592
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1522..1544
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1560..1576
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1632..1981
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1640..1658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1676..1694
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1712..1730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1748..1766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1784..1802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1820..1838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1856..1874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1920..1944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1988..1997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3092..3094,3095..3808)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3092..3094,3095..3805)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3134..3155)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3395..3414)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3742..3763
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3838..4337
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3873..3896)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4270..4289
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4358..4957
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4358..4377)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4854..4874
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5138..5563
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5666..5688)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5824..5844)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5829..6158
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6009..6144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6180..6199
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6280..6297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6451..7039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6531..6550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7210..8001,8002..8070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7833..7852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8071..8175)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8243..8261
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8361..8407,1..333)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
       61 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      121 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      181 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      241 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      301 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      361 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      421 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      481 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcaa
      541 taaaagagcc cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg
      601 gtacccgtat tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc
      661 cttgggaggg tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc
      721 tcgtccggga tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct
      781 ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc
      841 tgcgtctgta ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg
      901 agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt
      961 gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc
     1021 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
     1081 ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct
     1141 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     1201 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1261 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1321 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1381 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1441 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1501 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1561 tttatccagc cctcactcct tctctaggcg ccggaattga agatctcgat gaggcccttt
     1621 cgtcttcact cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct
     1681 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     1741 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     1801 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     1861 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga
     1921 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact
     1981 tggatccgcc accatggaca agctaaagaa ggtgctggac aaattgagat tgaaacgcaa
     2041 agatatctcg gaggcggccg agacggtgaa taaagttgtg gaacgcctgc tgcgcagaat
     2101 gcagaaacgg gagtcggagt tcaaaggtgt ggagcagctg aacactggca gctactatga
     2161 acatgtgaag atttctgctc ctaatgaatt tgatgttatg tttaaactgg aagtccccag
     2221 gattgagcta caagaatatt atgaaacagg tgctttctat cttgtgaaat tcaaaagaat
     2281 tccacgagga aatccgctga gtcatttctt agaaggggaa gtattatcag ctaccaagat
     2341 gctgtcaaag tttaggaaaa tcattaaaga agaagttaaa gaaatcaaag atatagatgt
     2401 cagtgtggag aaggaaaaac caggaagccc tgctgtaaca cttcttatca ggaaccctga
     2461 agaaatctct gtggatataa ttctggcttt ggagtcaaaa ggcagctggc ctattagtac
     2521 caaagaagga ctacctattc aaggctggct gggcacaaaa gtgaggacca atctaagacg
     2581 agagccgttt tatctcgtac ccaagaatgc aaaggatgga aatagttttc aaggagagac
     2641 ctggcgcctc tctttctctc acactgaaaa gtacattttg aataatcacg ggatagagaa
     2701 aacatgctgt gaatcttccg gagcaaaatg ctgcagaaaa gaatgtttaa aattaatgaa
     2761 ataccttttg gaacagttga aaaaagagtt tcaagagctg gatgcattct gttcctacca
     2821 tgtgaaaact gccatctttc acatgtggac ccaggacccg caggacagtc agtgggaccc
     2881 caggaacctc agctcctgct tcgataagtt gttagcattc tttcttgagt gcctcaggac
     2941 agagaaactg gatcattatt ttattccaaa gttcaatcta ttctctcaag aactaattga
     3001 ccgaaaaagt aaagaatttc tatcgaagaa aattgaatat gaaagaaata atgggtttcc
     3061 aatttttgac aagcttggtg gcggaggtag tgtgagcaag ggcgaggagc tgttcaccgg
     3121 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3181 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3241 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3301 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3361 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3421 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3481 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3541 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3601 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3661 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     3721 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3781 tctcggcatg gacgagctgt acaagtaagc gccgcgccgg cgggcccgaa ttctaccggg
     3841 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3901 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3961 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4021 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4081 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4141 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4201 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4261 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4321 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     4381 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4441 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4501 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4561 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4621 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4681 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4741 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4801 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4861 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4921 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     4981 cccgaccgaa aggagcgcac gaccccatgc atcggcgtct cgagatatca gtggtccagg
     5041 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     5101 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     5161 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     5221 tagagaagtt cagatcaagg tcaggaacag atggaacagg gtcgacccta gagaaccatc
     5281 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     5341 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     5401 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     5461 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     5521 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     5581 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg
     5641 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5701 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5761 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tagatccggc
     5821 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5881 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6001 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6061 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6121 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttactggc ttaactatgc
     6181 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6241 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7321 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7501 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     7561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7801 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     7861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7921 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     8281 agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
     8341 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     8401 ggggtca
//
