LOCUS       sequence-366116-       13328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High-yield production of coenzyme F420 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Last D, Hasan M, Rothenburger L, Braga D, Lackner G
  TITLE     High-yield production of coenzyme F420 in Escherichia coli by 
            fluorescence-based screening of multi-dimensional gene expression 
            space.
  JOURNAL   Metab Eng. 2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006.
  PUBMED    35863619
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006."
COMMENT     Sequence Label: pDL52 (clone45)
FEATURES             Location/Qualifiers
     source          1..13328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5032..5056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5181..5205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      8358..8404
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8441..8896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8528..8547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8738..8759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8989..9804)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8989..9804)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9712..9731
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9796..9815
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9926..10514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10415..10434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10668..10685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10700..10842
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10768..10787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10928..10950
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10944..11135)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11944..13026)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12988..13007
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13027..13104)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(13310..13328,1)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ctattatatc
       61 agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctatcccag
      121 gtttgcatca gatcgccatt ttccggtctg atccaacaag cctgcattcc gctgcgaatt
      181 gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg
      241 ttgagttttt ccgcagccaa aaagtacata tcgctgaacg gttttgagcg cccgtgcggg
      301 ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgtgcattg
      361 ccgttagtga tcgccaccag cggccatttc ttcgccagct gttttaaggt gtcgtgagtt
      421 tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt tgatcattgc tgcgtgtgcg
      481 cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga
      541 aaacgccagc gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc
      601 aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag
      661 gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc
      721 aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat cggtacgtct
      781 ttatccctat tatttgttca gcaaatggcc cattttctcg gctttggtat cgagataatg
      841 ttcgttattg gggttacgac ctacaatcaa tggtacgcgt tcaacaatat taatccctgc
      901 ttcggtcaga atttcgactt ttttcgggtt attggttaac aagcggactt cattgacgcc
      961 aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg cgaagcctaa
     1021 ctggtggtta gcctctacgg tatcgtaacc ttgatcctgc agtgcgtaag cgcggatttt
     1081 attcagcaga ccaatgttac gaccttcctg acggtgatac agcaaaatac cacggccttc
     1141 ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc gcaagctgaa
     1201 cagggcgtca ccggtcagac attcggaatg gacgcgcgca agtaccgggg tatgcccgga
     1261 aatatcgcca tagactagcg cgacatgatc gtgtccggtt gccagttctt caaatcccac
     1321 catcaggaaa tcgccccatg gggttggcag tttggcttct gccacacgtt taagctgcat
     1381 caacgtaccc cctgaaaaca cctattatgc acctactaaa tgcaggcaaa catccgggcc
     1441 tacatgacgt atctctttga atttaaattg gggggcgtcg gctaatttct caagccctgg
     1501 cagcgagcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag
     1561 ctcatcgact aaacccgcct gcagcaatgc gccagcgagc gttggccccg cttccaccca
     1621 gatgctgtta atttgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc
     1681 tttatgcgct ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg
     1741 cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg cgatgtaccg gcgtcacgcg
     1801 attttggcta tcaatcacaa tacgtatcgg ctgacggaga ttttgttgcg gatagagcgc
     1861 ctgagtttgt tcatccagtt cagaccaacg caccgttaag gcaggatcat ccgccagcac
     1921 cgtggcgctg ctggttaaaa tggcatgact ttgcgcgcgc agtagttgta catcacgccg
     1981 cgcctggggc gaagtgatcc actggctttc gccgctcgcc atcgccgtgc gaccatcaag
     2041 cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc
     2101 tttattcaat tgctcggctt cactcatcat caggccgtgg ctgacgtcaa tgccagcctg
     2161 ttgcagacgg taaagtccac gcccagcgac ctgcgggtta ggatcttgca tcgaggcaac
     2221 cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac gaccatgatg
     2281 gctacagggt tcgagtgtga cataggcggt cgcacctttg gctttttcac ccgccatacg
     2341 caacgcgtgt acttcggcat gtggttcacc cgcacgttgg tggtaacctt caccgacaat
     2401 ttcgccatct ttgacaatga cgcacccgac attcgggttg ggatgcgtgg taaaacgtcc
     2461 tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcatcc ctgaacgtcc
     2521 tttgatgacc tattactgtt cggcttcgct cagcacctga tgaccatgac cgcgaaccag
     2581 gcgacctgct ttggtaaaac gaaccggaac cagtgccgga gctgcatggc ttgcctgctg
     2641 acgttccggt gaaacattac gatacagcgg tgtacgctgc attgcattac gacccagacg
     2701 cgcagccagg gcttccattg ctgccggtgg catttcttgg ccatgtgctg cacccgctgc
     2761 acggctaatg ctttcattca tcagggtgcc acctaaatca tttgcaccgg cttgcaggca
     2821 cagttgtgca ccatgttcac ccattttaac ccagctcgtc tgaatattat caatcagacc
     2881 atgaaatgcc agacgaccaa cggcatgcat cagaattgct tcacgcagcg tcggaccctg
     2941 acgtgcacct ttctttttaa acagcggagc ttcttcatga acaaacggca gcggaacaaa
     3001 ttcggtcaga ccaccagtat cacgttgcag ttctgccagg cgcagaatat gacgtgccca
     3061 atgtttgtag ctttccacat gaccaaacat aatggtacag gtggtacgca gacccagttc
     3121 atgtgcgcta cgaaccacgg tcagccattc ttgggtattc agtttatcag gacaaatctg
     3181 gcgacgaact tcatcatcca ggatttccgc tgcggtgcca ggcagggtgc tcagacctgc
     3241 gcttttcaga tcactcagaa aggcgctaat gctggtgccc agtgtggttg caccatgcag
     3301 aacttccagc ggactaaatg catgaatgtg catatccgga cattcggttt ttgctgcttc
     3361 acaaatatcc agataggtac gacctgtata atccggatga ataccacctt gcagacaaac
     3421 ttcggttgct ccgcgatccc atgcttcttt aacacgacga cgaatttcat tcagatccac
     3481 aatatacggt ttttcacgca gatcctcttg aatcggacca cggctaaatg cacaaaagtt
     3541 gcagtggtac tgacaaacgt tggtataatt gatgttgcgg ttcacaacat aggtaatggt
     3601 atcacctttt gtctgacgac gcagacgatc tgcggtatgg gtcacatggg taaaatcatg
     3661 accacgtgca ttaaacaggt gaaccagttc tgcttcttcc agacgctcac cggcaacaca
     3721 tttttcaaca atatcaatgg tctggctacc aacacgatca acctggcttg cggtcggggt
     3781 aactgccaga catgcaatat caccaatggt ggtgctacct gctttccaaa tatcgctggt
     3841 cgccagaccg ctacaatcgc tatgacgcag aacatcggca tgcagacccg gatcaatcca
     3901 acgatcacgt tcttcaatat atgccggata cacggtaata cgttcggtca gggttttacc
     3961 accacgatcg gtatcttcac tcagacgatc cagatgaggc cacggtgctt caggattaac
     4021 atgatccggt gtaaccggac taacaccacc ccaatcatta atacctgcac gaatcagatc
     4081 ggtcagatcg ccatcaaaca ggttaggcgg aacctgaatg ctcattgcgc tacccagaac
     4141 cagacgggca actgctgtgg tccaacacag ttcatccatg ctcggttccg gtgcattaac
     4201 cattttcgta cccggttttg cacgaaagtt ctggataatc acttcttgaa catgaccata
     4261 acgatcatgc agatcacgca gtgcaaacag gctttccagg cgttcacgac gggtttcacc
     4321 aataccaatc agaataccgc tggtaatcgg aatatgtgct tcgcctgcca gacgcagggt
     4381 ttccagacgt gctgccggat gtttatccgg actaccataa tgcggaccac cacgttcgct
     4441 cagacgttcg cttgcggttt ccagcatcag accaaagctc ggtgcatgcg gacgcagcat
     4501 ctgatattct gctgcgctca gaacacccat gttaaaatgc ggcagcagac ctgtttctgc
     4561 acgaacacgc tgtgcaactt cggcaacata atctgcggtg gttgcatgac ccagttgttg
     4621 cagtgcatca cgtgctgctg cataacgtgc ttccggacga tcacccaggg taaacagtgc
     4681 ttcacgacaa ccaactgctg caccggcacg tgcaatatcc agtgcctgat caacggtcag
     4741 aaatgcctga ccaataacac gcggtgcttt tgcaaaggtg caataatgac aaacatcacg
     4801 acacagatgt gtcagcggga taaaaacttt gcggctatag gtaataacat taccccaacc
     4861 aatatcacgc catgcgcttg ccagacctgc cagttcacgc caatcctgaa ctgcttccag
     4921 ttcataggcc agtgctttat catccagacg aggctggcta ccttttgcgc tacgttcaac
     4981 ttcttgaatc atgtttggac ctacctaggt tgacaaaatt atttctagag gggaattgtt
     5041 atccgctcac aattccccta tagtgagtcg tatgaattta agcttcgcgg gatcgagatc
     5101 tcgcagacta gttctgacat gccagtcggc gtagaggatc gagatctcga tcccgcgaaa
     5161 tttatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
     5221 ttgtttaaac ttcgcggtat tattatgaac tgcggtatca aaatgaaagt gattattccg
     5281 gttagcccga tcaatagcct gaaaacccgt ctgagcgaat ttctgagcgg tgaagaacgt
     5341 aaaaatctgc tgctgaatat gctgaaggat atcattaaag cactggatgg tctggatatt
     5401 gttattgtta gccgtgatga agagatcctg gattttgcca aaaatgaact gaaagccgaa
     5461 accatcaaag agaaatacaa aggcctgaac aacgcaatca aacaggcgtt tgaagaaatc
     5521 gaagataaag aggtgattat cattccggca gatattccgc tgatcaaaaa aaagcacatc
     5581 gaggacattc tgaagctgag caaaaactat gatctgatta ttgcaccgag ccgtggtggt
     5641 ggcaccaatc tgctgtatct gaaaagcaaa gatctgatcg agatcaaata tgagggcttc
     5701 agctttctga aacatctgga agaagccaaa aaacgcaatc tgcgctatta tatctatgat
     5761 agcttcctga ttagcgtgga tatcaataca ccggaagatc tgggtgaaat ctttattcat
     5821 ggcaacgata cctacaccaa aaactacctg aaaagcctgg gtattgatgt ggaaccgaaa
     5881 cattcaagcg caggtcgttt cgttgttaaa cgtcgttaat aggaacgcta cggaggtgca
     5941 cccatgattt ttgtgattac cgttctgagc ggtggtacag gcaccccgaa actgctgcag
     6001 ggtctgaaac gtgttgttaa taatgaagaa ctggccgtga ttgtgaatac cggtgaagat
     6061 acctggattg gtgatctgta tctgagtccg gatgttgata ccgtgctgta taccctggca
     6121 gatctgatta acgaagaaac ctggtatggt gtgaaagagg atacctttta tacccacgaa
     6181 cagctgaaaa atctgggctt tgatgaagtt ctgcgcattg gtgataaaga tcgtgcactg
     6241 aaaatgcaca aaacctatta tctgaaacgc ggtcataaac tgagcgaagt tgttgatatg
     6301 gaaaaagttg ccctgggcat taaagcaaaa gttattccga tgaccgatga tcgtgtggaa
     6361 accaaaattc tggcaaaagt tgatggtaaa gtggacctgc tgaaattcca tgatttttgg
     6421 gttaaacgca aaggtgatgt tgaagtgctg gatgtgattt atgaaaacag cctgtatgca
     6481 aaaccgtgcg aaaaagcagt tgaagccatt aaaaacagcg atctggttat tattggtccg
     6541 agcaatccga ttaccagcat cggtccgatt ctgagcctga atggtattaa agaactgctg
     6601 aaagacaaaa aagttgttgt ggttagcccg attgttggta atagcgcagt tagcggtccg
     6661 gcaggtaaac tgatgaaagc caaaggttat gatgttagcg tgaaaggcat ctacgagttc
     6721 tataaagata ttgtggatgt gctggtgatc gacaacgtgg ataaagaaat tgcaaaagaa
     6781 attccgtgcg aagtgctgat taccaatacc atcatgaaaa ccctggatga taaagttcgt
     6841 ctggccaaaa acatcattga attttgtggt agcctgtaat agcaaagtaa ggagggcaag
     6901 tcatgtctca ccacgacgcg gttccgccgc cgctggttcc gctgccggac ccggaccgtg
     6961 ttgcgggttc tcgtccgctg gacctgccgg aaatcatccg tggtcgtcgt tctgttcgtc
     7021 gtctgcgtac cgacccggtt gcgccggaac tggttaccgc ggttctggaa gcggcggcgt
     7081 gggcgccgtc tccgcacggt acccagccgt ggcgtttcgt tgttctgacc cagccggaac
     7141 gtaaacgtca actggcggac gcgatggcga ccgcgtggcg tcaccacctg gcgatggacg
     7201 gtcaggacga agaaaccatc gcgcgtcgtc tggcgggttc tcagcgtcgt ctgtgcgaag
     7261 cgccggttct gatcctggtt tgcctggacc cgtctgacct ggaccgttac ccggacccgg
     7321 aacgtcaggc ggcggaacgt actatggcga tccagtctct gggtgcggcg atccagaacc
     7381 tgctgctgat ggcgtaccgt ctgggtctgg acaccggctg gatgtgcgcg ccgctgttct
     7441 gcccggcggt tgttcgtgaa atcctggacc tgccgccggt tctggaaccg caggcgctgg
     7501 ttaccctggg ttacgcggcg gcggacccgc agcgtcgtcc gcgtcgtccg ctgcaccaac
     7561 tgatcgttcg ttgggaataa taggattgtg atataggagg taagatgatc aaggaaaagc
     7621 gcaaagttga agtgattggt ctggaactgc cgatttttaa aggcggcgaa cagattaatc
     7681 tgagtgaact gattgcccag tatccgattg aagatggtga cattattgtt attgcagaaa
     7741 ccctgattag caaactggaa ggtggtgtta ttgatcgtga taaaattatt ccgagcaaag
     7801 aagccattga actggccaaa aagactggta aagatccgaa agttgttcag gttattctgg
     7861 atgaagccaa agaaattgtg aaagttggca aaaatttcat catcaccgaa accaaacatg
     7921 gctttgtttg cgccaatagc ggtgtggatg aaagcaatat ctataaaggt attaagatcc
     7981 tgccgaaaaa tccggatgaa agtgccgaaa aaattcgcaa agaaattgaa aaactgaccg
     8041 gcaaacgcgt tggcgttatt attagtgata gcgtgggtcg cccgtttcgc aaaggcgcag
     8101 ttggtattgc cattggcgtt agcggcattc tggcactgtg ggatcgcaaa ggtgaaaaag
     8161 atctgtttgg tcgtgaactg aaaaccaccg aagtggccat tgccgatgaa ctggccagca
     8221 tggccaatgt ggttatgggc gaagccgatg aaggcattcc ggttgttatt attcgtggcg
     8281 caaatgtgcc gtttggcaat ggcaaaggcc gtgatctgat tcgtccgaaa gaagaagatg
     8341 tgtttcgcaa ttaatagtag cataacccct tggggcctct aaacgggtct tgaggggttt
     8401 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
     8461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8641 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8761 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8821 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8881 ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8941 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     9001 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     9061 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     9121 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     9181 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     9241 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     9301 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     9361 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     9421 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     9481 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     9541 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     9601 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     9661 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     9721 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga
     9781 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     9841 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9901 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9961 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10021 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    10081 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10141 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10201 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10261 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10321 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10381 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10441 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10501 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10561 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10621 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10681 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    10741 cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10801 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    10861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    10921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    10981 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
    11041 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
    11101 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
    11161 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
    11221 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
    11281 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
    11341 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
    11401 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
    11461 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
    11521 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
    11581 agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg
    11641 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
    11701 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
    11761 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
    11821 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
    11881 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
    11941 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    12001 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
    12061 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
    12121 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat
    12181 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg
    12241 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag
    12301 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat
    12361 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt
    12421 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc
    12481 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg
    12541 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac
    12601 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat
    12661 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc
    12721 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat
    12781 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag
    12841 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
    12901 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac
    12961 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg
    13021 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa
    13081 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca
    13141 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
    13201 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
    13261 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
    13321 cgatatag
//
