LOCUS       sequence-366120-        2367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fragment template for DAP array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Yuan Q, Gao X
  TITLE     Multiplex base- and prime-editing with drive-and-process CRISPR 
            arrays.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1.
  PUBMED    35589728
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2771. doi"; pages: " 
            10.1038/s41467-022-30514-1"
COMMENT     Sequence Label: hCtRNA_FT
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(374..478)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        585..833
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     585..605
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     756..775
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        854..929
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(1121..1709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1201..1220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1880..2367,1..304,305..373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
       61 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      481 catttccccg aaaagtgcca cctgacgtcg ctagctgtac aaaaaagcag gctttaaagg
      541 aaccaattca gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca
      601 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
      661 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
      721 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgagtccg
      841 agcagaagaa gaagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      901 aacttgaaaa agtggcaccg agtcggtgca gagggggtat agctcagtgg tagagcattt
      961 gactgcagat caagaggtcc ccggttcaaa tccgggtgcc ccctggaatc ccttctgcag
     1021 caccaagctt gggccgctcg aggtacctct ctacatatga catgtgagca aaaggccagc
     1081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1561 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc tcaccggctc
     2041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2341 tggccgcagt gttatcactc atggtta
//
