LOCUS       sequence-366122-        2096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector template for DAP array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Yuan Q, Gao X
  TITLE     Multiplex base- and prime-editing with drive-and-process CRISPR 
            arrays.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1.
  PUBMED    35589728
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2771. doi"; pages: " 
            10.1038/s41467-022-30514-1"
COMMENT     Sequence Label: hCtRNA_VT
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(374..478)
                     /gene="bla"
                     /label=AmpR promoter
     misc_RNA        671..746
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(850..1438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(930..949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1609..2096,1..304,305..373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
       61 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      481 catttccccg aaaagtgcca cctgacgtcg ctagctgtac aaaaaagcag gctttaaagg
      541 aaccaattca gtcgactgga tccggtacca aggtcagagg gggtatagct cagtggtaga
      601 gcatttgact gcagatcaag aggtccccgg ttcaaatccg ggtgccccct gagtccgagc
      661 agaagaagaa gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      721 ttgaaaaagt ggcaccgagt cggtgctttt tttaagcttg ggccgctcga ggtacctctc
      781 tacatatgac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1741 gataccgcga gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggtta
//
