LOCUS       sequence-366123-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ACME dual-deaminase CRISPR base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Yuan Q, Gao X
  TITLE     Multiplex base- and prime-editing with drive-and-process CRISPR 
            arrays.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1.
  PUBMED    35589728
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2771. doi"; pages: " 
            10.1038/s41467-022-30514-1"
COMMENT     Sequence Label: pCMV-ACME
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        270..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        650..853
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     804..824
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             881..928
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1523..1771
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             1802..2395
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="EASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSV
                     KMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWG
                     CAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDT
                     FVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             join(2492..2515,2516..2518,2519..6592)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2492..6592
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6593..6640
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6649..6666
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6689..6706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6695..6919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6990..7006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6990..7006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7123..7140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7294..7882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7374..7393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8053..8844,8845..8913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8676..8695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8914..9018)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttacc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcaga gctctctggc taactaccgg tgccaccatg aaaagacctg ccgctacaaa
      901 gaaggccggc caggccaaga agaaaaagtc tgaggtggag ttttcccacg agtactggat
      961 aagacatgcc ctgaccctgg ccaagagggc acgggatgag agggaggtgc ctgtgggagc
     1021 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     1081 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     1141 ctacagactg attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg
     1201 cgccatgatc cactctagga tcggccgcgt ggtgtttggc tggaggaact caaaaagagg
     1261 cgccgcaggc tccctgatga acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat
     1321 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgcgatttct atcggatgcc
     1381 tagacaggtg ttcaatgctc agaagaaggc ccagagctcc atcaactccg gaggatctag
     1441 cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac cagagagcag
     1501 tggcggcagc agcggcggca gcactaatct gagcgacatc attgagaagg agactgggaa
     1561 acagctggtc attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg
     1621 caacaagcca gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa
     1681 tgtgatgctg ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga
     1741 ttctaacggc gagaataaga tcaagatgct gagcggagga tccggaggat ctggaggcag
     1801 cgaagccagc ccagcatccg ggcccagaca cttgatggat ccacacatat tcacttccaa
     1861 ctttaacaat ggcattggaa ggcataagac ctacctgtgc tacgaagtgg agcgcctgga
     1921 caatggcacc tcggtcaaga tggaccagca caggggcttt ctacacaacc aggctaagaa
     1981 tcttctctgt ggcttttacg gccgccatgc ggagctgcgc ttcttggacc tggttccttc
     2041 tttgcagttg gacccggccc agatctacag ggtcacttgg ttcatctcct ggagcccctg
     2101 cttctcctgg ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca cacacgtgag
     2161 actgcgtatc ttcgctgccc gcatcttcga ttacgacccc ctatataagg aggcactgca
     2221 aatgctgcgg gatgctgggg cccaagtctc catcatgacc tacgatgaat ttaagcactg
     2281 ctgggacacc tttgtggacc accagggatg tcccttccag ccctgggatg gactagatga
     2341 gcacagccaa gccctgagtg ggaggctgcg ggccattctc cagaatcagg gaaactctgg
     2401 aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt cagcaacacc
     2461 agagagcagt ggcggcagca gcggcggcag cgacaagaag tacagcatcg gcctggccat
     2521 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     2581 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     2641 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     2701 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     2761 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     2821 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     2881 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     2941 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     3001 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     3061 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     3121 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     3181 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     3241 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     3301 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     3361 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     3421 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     3481 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     3541 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     3601 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     3661 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     3721 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     3781 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     3841 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     3901 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     3961 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     4021 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     4081 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     4141 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     4201 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     4261 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     4321 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     4381 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     4441 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     4501 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     4561 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     4621 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     4681 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     4741 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     4801 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     4861 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     4921 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     4981 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     5041 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     5101 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     5161 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     5221 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     5281 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     5341 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     5401 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     5461 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     5521 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     5581 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     5641 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     5701 gatcgtatgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     5761 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     5821 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     5881 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     5941 gggcaagtcc aaaaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     6001 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     6061 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     6121 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     6181 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     6241 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     6301 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     6361 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     6421 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     6481 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     6541 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg acaaaaggcc
     6601 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taaccggtca tcatcaccat
     6661 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6721 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6781 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6841 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6901 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     6961 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgattg cgttgcgctc
     7021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacactca
     7921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8161 accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt
     8221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9001 acaaataggg gttccgcg
//
