LOCUS       sequence-366129-        6539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrative plasmid for expressing deltaVP1, an NLS deletion 
            mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine 
            auxotrophic marker (K. lactis LEU2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Cheah LC, Liu L, Plan MR, Peng B, Lu Z, Schenk G, Vickers CE, 
            Sainsbury F
  TITLE     Synthetic in vivo compartmentalisation improves metabolic flux and 
            modulates the product profile of promiscuous enzymes
  JOURNAL   bioRxiv 2022.11.24.517869
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.11.24.517869"
COMMENT     Sequence Label: pGAL1-VP1 (LEU2)
FEATURES             Location/Qualifiers
     source          1..6539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2153..2594
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2153..2270
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2565..2588
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      3986..4233
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4002..4020)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4247..4654)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(4668..4685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4839..5427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4919..4938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5598..6389,6390..6458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6221..6240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6459..6539,1..24))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcattta
       61 aatcaatatt aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca
      121 atttgcttac ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag
      181 attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt
      241 tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt
      301 ttaagccaag atttccttga cagccttggc gatagcatcg ccaacctcag tggtagagtt
      361 agaaccacca aggtcaccgg ttctgacacc tgcatccaag acatttctaa cagcttcttc
      421 aagagcccta ccttcttcaa ccaaatccaa ggataacttc aacatcatag ctgcagataa
      481 gatggtagca attgggttaa ccttgtttgc tggtaaatct ggggcagaac catgacatgg
      541 ttcgtacaaa ccgaatgcct tgttagtgtc aggtagggaa gctagagatg cagaaggtaa
      601 taaacccaaa gaacctggaa taacagaggc ttcatcggag ataatatcac caaacatgtt
      661 gttggtaata acaacaccgt ttagcttagt tggtgattta accaaaatca tagcagcaga
      721 gtcgatcaat tggtgctgaa cagttaattg tgggaactca gtcttgatgg tttcttcaac
      781 agtctttctc cacaatctgg aagaggcaag cacgttagcc ttgtcaagag accagattgg
      841 taatggtggg ttttgttgca atgccaagaa agcagccatt cttgtaattc tttgaacttc
      901 aggaacactg tatttctcag agtcccaagc aactccgtca ccttcatctt cttttctttc
      961 accaaagtag ataccaccaa ccaattctct aacgacgacg aaatcggtac cctttgcata
     1021 ttcaggcttc aaaggagaaa gatctagtaa agaatcagaa gcaaagttac atggtctcaa
     1081 gttggcgtat agacccaatt cctttctgat cttcaataga ccttgttctg gtctaactgc
     1141 gcccgtaccc cattttggac caccaacagc acctagtaag acagcatcgg ctttcttaga
     1201 ggcttctaga gcttcatctg gtaaaggagt gccagtggca tcgatggcag cacccccgat
     1261 caagtgatgt tggaaattga acttaatttc tggacggact tcagcaatgg cattcaagac
     1321 cttaatagct tcgtcagtaa cttctttacc gacgtgatca cccggtagga caacgatatt
     1381 cttagacatg gtataatctg tgtagtgtgg gatacttttt acttcttcaa ataggtatca
     1441 acaataaaaa taaatcgaag caaatgtagg aatgcgttaa agcagatgta cttacttaga
     1501 gtacatagat atatttatat aattcaatat ataaaagtat atgaagcatc tgatgttgaa
     1561 cctgtcatga ctctaaactg gaatgggtag ttatgcttct gaaggttttc cgttagaaaa
     1621 tgagtgcagg gatcaggccc ggaaccggtt ttagcctgaa aggaaaagaa aaaaatgcgg
     1681 tgattactag cacgtgactg cgctgaattg gaatctacca caagtacagc gaatggcaag
     1741 agggaagggg aatatattgt gcctggcggt agcgtctgct atatcaaatg ttaaacataa
     1801 ctaaatgcag tagtgaatag agtaatgtat ttctacatat gtgggtaggg ccaatgggag
     1861 cccgatgtct attgtacaga tattttcctt ttatagttaa tcaattcaac ttggaaatca
     1921 ttgagtcgat gaattcttat attgaatttt caaaaattct tacttttttt tttggatgga
     1981 cgcaaagaag tttaataatc atattacatg gcaataccac catatacata tccatatcta
     2041 atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt
     2101 ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag
     2161 aagccgccga gcgggcgaca gccctccgac ggaagactct cctccgtgcg tcctggtctt
     2221 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag
     2281 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca
     2341 caaaccttca aatcaacgaa tcaaattaac aaccatagga taataatgcg attagttttt
     2401 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata
     2461 tataaatgca aaagctgcat aaccacttta actaatactt tcaacatttt cggtttgtat
     2521 tacttcttat tcaaatgtca taaaagtatc aacaaaaaat tgttaatata cctctatact
     2581 ttaacgtcaa ggagaaaaaa ctatagctag caaaatggct tcaggtgtaa gtaaatgcga
     2641 aaccaaatgc acaaaggctt gccctagacc agctccagtt cctaagttat tgattaaagg
     2701 tggtatggaa gttttggatt tggttacagg tccagattct gttactgaaa tcgaagcatt
     2761 tttgaaccca agaatgggtc aaccaccaac accagaatct ttaactgaag gtggtcaata
     2821 ttacggttgg tcaagaggta ttaatttggc tacatctgat actgaagatt caccaggtaa
     2881 taatacatta ccaacttggt ctatggcaaa attgcaatta ccaatgttga acgaagattt
     2941 gacttgtgat actttgcaaa tgtgggaagc agtttcagtt aaaacagaag ttgttggttc
     3001 tggttctttg ttggatgttc atggttttaa taagccaaca gatactgtta acacaaaggg
     3061 tatttctact ccagttgaag gttcacaata tcatgttttt gctgttggtg gtgaaccatt
     3121 ggatttgcaa ggtttagtta cagatgcaag aactaagtac aaggaagaag gtgttgttac
     3181 tattaaaaca atcactaaga aagatatggt taataaggat caagttttga acccaatttc
     3241 aaaggctaaa ttggataagg atggcatgta cccagttgaa atttggcatc cagatccagc
     3301 taaaaatgaa aacacaagat acttcggtaa ttacactggt ggtactacaa ctccaccagt
     3361 tttgcaattc actaacactt tgacaactgt tttgttagat gaaaatggtg ttggtccatt
     3421 atgtaaaggt gaaggtttgt acttatcttg cgttgatatt atgggttgga gagttacaag
     3481 aaactacgat gttcatcatt ggagaggttt gccaagatac ttcaagatta ctttaagaaa
     3541 aagatgggtt aaaaatccat acccaatggc ttctttgatt tcttctttgt ttaataatat
     3601 gttaccacaa gttcaaggtc aaccaatgga aggtgaaaac acacaagttg aagaagttag
     3661 agtttacgat ggtactgaac cagttccagg tgaccctgac atgacaagat atgtagatag
     3721 attcggtaaa actaaaactg tattccctgg taattaagcg gccgcactag taggactatt
     3781 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     3841 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     3901 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg
     3961 tcgtaatgcc tgcaggaggg ccgcatcatg taattagtta tgtcacgctt acattcacgc
     4021 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     4081 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     4141 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     4201 aaggttttgg gacgctcgaa ggctttaatt tgcggcctgc ggccgctaga atggtatatc
     4261 cttgaaatat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag
     4321 acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca
     4381 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
     4441 ccggcgctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca
     4501 ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg
     4561 cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc
     4621 ttgcatctta cgatacctga gtattcccac agttatttaa atgcttcctc gctcactgac
     4681 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4741 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4801 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4861 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4921 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4981 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5041 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5101 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5161 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5221 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5281 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5341 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5401 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5461 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5521 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5581 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5641 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5701 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5761 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5821 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5881 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5941 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6001 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6061 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6121 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6181 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6241 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6301 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6361 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6421 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6481 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaa
//
