LOCUS       sequence-366132-        2426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Cre from CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2426)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENN.AAV.CMVs.Pl.Cre.rBG
FEATURES             Location/Qualifiers
     source          1..2426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    212..267
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     313..332
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(410..1441)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     promoter        complement(1492..1510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1553..1572)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          1555..1687
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(1802..1826)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1823..2026)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1852..1872)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgctcact gaggccggsc grscaaagsy cgsscgwcgs scgrsctttg sycgsscggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
      121 gtagttaatg attaacccgc catgctactt atctaccagg gtaatgggta ttatgggtgg
      181 tcgaaattca gatcaacgaa ttgcttccga gtgagagaca caaaaaattc caacacacta
      241 ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga
      301 tgtccccata atttttggca gagggaaaaa gatcggatcc tcaggcgtag ttcaccccgt
      361 cctcgaggcc gcccgggtcg actctagagg taccacgccg cgttaatggc taatcgccat
      421 cttccagcag gcgcaccatt gcccctgttt cactatccag gttacggata tagttcatga
      481 caatatttac attggtccag ccaccagctt gcatgatctc cggtattgaa actccagcgc
      541 gggccatatc tcgcgcggct ccgacacggg cactgtgtcc agaccaggcc aggtatctct
      601 gaccagagtc atccttagcg ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca
      661 gggcgcgagt tgatagctgg ctggtggcag atggcgcggc aacaccattt tttctgaccc
      721 ggcaaaacag gtagttattc ggatcatcag ctacaccaga gacggaaatc catcgctcga
      781 ccagtttagt tacccccagg ctaagtgcct tctctacacc tgcggtgcta accagcgttt
      841 tcgttctgcc aatatggatt aacattctcc caccgtcagt acgtgagata tctttaaccc
      901 tgatcctggc aatttcggct atacgtaaca gggtgttata agcaatcccc agaaatgcca
      961 gattacgtat atcctggcag cgatcgctat tttccatgag tgaacgaacc tggtcgaaat
     1021 cagtgcgttc gaacgctaga gcctgttttg cacgttcacc ggcatcaacg ttttcttttc
     1081 ggatccgccg cataaccagt gaaacagcat tgctgtcact tggtcgtggc agcccggacc
     1141 gacgatgaag catgtttagc tggcccaaat gttgctggat agtttttact gccagaccgc
     1201 gcgcctgaag atatagaaga taatcgcgaa catcttcagg ttctgcggga aaccatttcc
     1261 ggttattcaa cttgcaccat gccgcccacg accggcaaac ggacagaagc attttccagg
     1321 tatgctcaga aaacgcctgg cgatccctga acatgtccat caggttcttg cgaacctcat
     1381 cactcgttgc atcgaccggt aatgcaggca aattttggtg tacggtcagt aaattggaca
     1441 tttcacactc agataatggt tttaagtaaa gtgtacaatt ctcgaggcta gcctatagtg
     1501 agtcgtatta agtactctag ccttaagagc tgtaattgaa ctgggagtgg acacctgtgg
     1561 agagaaaggc aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc
     1621 ttctctgtct cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga
     1681 tacttacctg cccagtgcct cacgaccaac ttctgcagct taagttcgag actgttgtgt
     1741 cagaagcact gactgcgtta gcaatttaac tgtgataaac taccgcaata aagcttctag
     1801 tgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     1861 cgcccatttg cgtcaacggg gcggggttat tacgacattt tggaaagtcc cgttgatttt
     1921 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     1981 caaaccgcta tccacgccca ttggtgtact gccaaaaccg catcaccatg gtaatagcga
     2041 tgactaatac gtagatgtac tgccaagtag gaaagtcccg taaggtcatg tactgggcat
     2101 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcggactt ggcatatgat
     2161 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     2221 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     2281 ggtcgttggg cggtcagcct gcaggcatgc tagctatagt tctagaggat ccccattacc
     2341 ctggtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     2401 ttggccactc cctctctgcg cgctcg
//
