LOCUS       sequence-366146-       11404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MS2 coat protein fused with p65 and hsf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Chan WF, Coughlan HD, Chen Y, Keenan CR, Smyth GK, Perkins AC, 
            Johanson TM, Allan RS
  TITLE     Activation of stably silenced genes by recruitment of a synthetic 
            de-methylating module.
  JOURNAL   Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4.
  PUBMED    36151095
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-23"; volume: "13(1):5582. doi"; pages: " 
            10.1038/s41467-022-33181-4"
COMMENT     Sequence Label: MS2-p65-HSF1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..11404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..70)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        55..384
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      235..370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     297..316
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        432..479
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             498..872
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1002..1123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1039..1058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1093..1112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1172..1188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1172..1188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1185..1207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1196..1212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1220..1226,1227..1244,1245..1250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1265..1286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1403..1420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1574..2162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1654..1673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2333..3124,3125..3193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2956..2975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3194..3298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3373..3392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3564..3943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3945..4143
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4098..4118
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4161..4341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4388..4513
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5006..5239
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5424..5468
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5766..5883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6008..7186
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6239..7177
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7134..7154
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7193..7202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7202..7588
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7643..7663
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7679..8221
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8246..8617
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             8633..8686
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8687..9397
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8832..8850)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9100..9119
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9422..10010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9475..9495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9893..9904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10013..10029)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10014..10030)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10535..10715
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10741..10758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10747..10971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11017..11404,1..41)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11104..11123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11314..11335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
       61 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      301 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      361 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      421 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
      481 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
      541 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
      601 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
      661 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
      721 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
      781 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
      841 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
      901 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
      961 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     1021 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     1141 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3301 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     3361 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     3421 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     3481 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     3541 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     3601 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3661 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3721 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3781 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3841 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3901 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3961 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4021 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     4081 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4141 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4321 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     4381 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     4441 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     4501 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     4561 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     4621 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     4681 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     4741 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     4801 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     4861 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     4921 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     4981 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     5041 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     5101 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     5161 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     5221 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     5281 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     5341 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     5401 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     5461 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     5521 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     5581 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     5641 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     5701 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     5761 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     5821 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     5881 tttcgggttt attacaggga cagcagagat ccagtttggt taattaatgc aaagatggat
     5941 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     6001 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     6061 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     6121 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     6181 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     6241 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     6301 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     6361 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     6421 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     6481 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     6541 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     6601 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     6661 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     6721 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     6781 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     6841 atgaaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     6901 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     6961 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     7021 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     7081 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     7141 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat
     7201 ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt gggacagggg atgtgacagt
     7261 ggctccttct aatttcgcta atggggtggc agagtggatc agctccaact cacggagcca
     7321 ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc cagaagagaa agtataccat
     7381 caaggtggag gtccccaaag tggctaccca gacagtgggc ggagtcgaac tgcctgtcgc
     7441 cgcttggagg tcctacctga acatggagct cactatccca attttcgcta ccaattctga
     7501 ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa gacggtaatc ctatcccttc
     7561 cgccatcgcc gctaactcag gtatctacag cgctggagga ggtggaagcg gaggaggagg
     7621 aagcggagga ggaggtagcg gacctaagaa aaagaggaag gtggcggccg ctggatcccc
     7681 ttcagggcag atcagcaacc aggccctggc tctggcccct agctccgctc cagtgctggc
     7741 ccagactatg gtgccctcta gtgctatggt gcctctggcc cagccacctg ctccagcccc
     7801 tgtgctgacc ccaggaccac cccagtcact gagcgctcca gtgcccaagt ctacacaggc
     7861 cggcgagggg actctgagtg aagctctgct gcacctgcag ttcgacgctg atgaggacct
     7921 gggagctctg ctggggaaca gcaccgatcc cggagtgttc acagatctgg cctccgtgga
     7981 caactctgag tttcagcagc tgctgaatca gggcgtgtcc atgtctcata gtacagccga
     8041 accaatgctg atggagtacc ccgaagccat tacccggctg gtgaccggca gccagcggcc
     8101 ccccgacccc gctccaactc ccctgggaac cagcggcctg cctaatgggc tgtccggaga
     8161 tgaagacttc tcaagcatcg ctgatatgga ctttagtgcc ctgctgtcac agatttcctc
     8221 tagtgggcag ggaggaggtg gaagcggctt cagcgtggac accagtgccc tgctggacct
     8281 gttcagcccc tcggtgaccg tgcccgacat gagcctgcct gaccttgaca gcagcctggc
     8341 cagtatccaa gagctcctgt ctccccagga gccccccagg cctcccgagg cagagaacag
     8401 cagcccggat tcagggaagc agctggtgca ctacacagcg cagccgctgt tcctgctgga
     8461 ccccggctcc gtggacaccg ggagcaacga cctgccggtg ctgtttgagc tgggagaggg
     8521 ctcctacttc tccgaagggg acggcttcgc cgaggacccc accatctccc tgctgacagg
     8581 ctcggagcct cccaaagcca aggaccccac tgtctccgct agcggcagtg gagagggcag
     8641 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaatgg tgagcaaggg
     8701 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     8761 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     8821 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     8881 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     8941 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     9001 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     9061 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     9121 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     9181 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     9241 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     9301 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     9361 ccactccacc ggcggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga
     9421 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9541 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9601 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9661 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9721 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9781 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     9841 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9901 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     9961 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
    10021 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
    10081 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
    10141 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
    10201 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
    10261 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
    10321 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
    10381 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
    10441 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
    10501 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
    10561 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10621 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10681 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
    10741 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10801 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10861 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10921 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10981 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
    11041 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    11101 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    11161 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    11221 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    11281 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    11341 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11401 attg
//
