LOCUS       sequence-366150-        4820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence of human FAM3B pCS2 with C terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Zhang F, Zhu X, Wang P, He Q, Huang H, Zheng T, Li Y, Jia H, Xu L, 
            Zhao H, Colozza G, Tao Q, De Robertis EM, Ding Y
  TITLE     The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior 
            development in Xenopus.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2100342118. doi:
            10.1073/pnas.2100342118.
  PUBMED    33975953
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-18"; volume: "118(20). pii: 
            2100342118. doi"; pages: " 10.1073/pnas.2100342118"
COMMENT     Sequence Label: pCS2-hFAM3B-HA
FEATURES             Location/Qualifiers
     source          1..4820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             793..819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    859..993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(883..902)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     937..956
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1106..1126)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1106..1124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1145..1161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1145..1161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1158..1180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1169..1185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1193..1199,1200..1217,1218..1223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1238..1259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1547..2135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1627..1646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2306..3097,3098..3166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2929..2948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3167..3271)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3297..3752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3434..3455)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3646..3665
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3823..4807
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 ggggacgtcg gagcaagctt gatttaggtg acactataga atacaagcta cttgttcttt
       61 ttgcaggatc ccatcgatac catgcgccca ttggctggtg gcctgctcaa ggtggtgttc
      121 gtggtcttcg cctccttgtg tgcctggtat tcggggtacc tgctcgcaga gctcattcca
      181 gatgcacccc tgtccagtgc tgcctatagc atccgcagca tcggggagag gcctgtcctc
      241 aaagctccag tccccaaaag gcaaaaatgt gaccactgga ctccctgccc atctgacacc
      301 tatgcctaca ggttactcag cggaggtggc agaagcaagt acgccaaaat ctgctttgag
      361 gataacctac ttatgggaga acagctggga aatgttgcca gaggaataaa cattgccatt
      421 gtcaactatg taactgggaa tgtgacagca acacgatgtt ttgatatgta tgaaggcgat
      481 aactctggac cgatgacaaa gtttattcag agtgctgctc caaaatccct gctcttcatg
      541 gtgacctatg acgacggaag cacaagactg aataacgatg ccaagaatgc catagaagca
      601 cttggaagta aagaaatcag gaacatgaaa ttcaggtcta gctgggtatt tattgcagca
      661 aaaggcttgg aactcccttc cgaaattcag agagaaaaga tcaaccactc tgatgctaag
      721 aacaacagat attctggctg gcctgcagag atccagatag aaggctgcat acccaaagaa
      781 cgaagcgatc cttacccata cgacgtccca gactacgctt aatcgagcct ctagaactat
      841 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
      901 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      961 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1021 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     1081 tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     1141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1201 gagccgggag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2281 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2341 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2401 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2461 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2521 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2581 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2641 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2701 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2761 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2821 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2881 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2941 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3001 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3061 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3121 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3181 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3241 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     3301 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3361 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3421 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3481 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3541 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3601 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3661 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3721 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     3781 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgaccg ccaattcaat
     3841 atggcgtata tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat
     3901 atggcgtata tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat
     3961 atggcggact tggcaccatg ccaattcaat atggcggacc tggcactgtg ccaactgggg
     4021 aggggtctac ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg
     4081 ccatattgaa ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa
     4141 tatataggca atatccaata tggccctatg ccaatatggc tattggccag gttcaatact
     4201 atgtattggc cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag
     4261 cccctcccaa tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc
     4321 actcccccat tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc
     4381 ggtcctattg acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc
     4441 tggctcaatg cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc
     4501 attgcccatt catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca
     4561 cttggcagta catcaatatc tattaatagt aacttggcaa gtacattact attggaagta
     4621 cgccagggta cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg
     4681 ctgccaagta catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc
     4741 attgacgtaa atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt
     4801 agggaaccgc cattctgcct
//
