LOCUS       sequence-366158-        8405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of human cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTRE3G-GFP-human cGASdeltaN
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        339..542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             543..718
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    781..980
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1181..1597
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1527..1549
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1565..1581
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1637..1986
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1645..1663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1681..1699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1717..1735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1753..1771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1825..1843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1861..1879
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1925..1949
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1993..2002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1999..2001,2002..2004,2005..2715)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2044..2065)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2305..2324)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2652..2673
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3853..4340
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3888..3911)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4273..4292
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4361..4960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4361..4380)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4857..4877
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5141..5566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5669..5691)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5827..5847)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5832..6161
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6012..6147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6183..6202
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6283..6300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6454..7042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6534..6553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7213..8004,8005..8073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7836..7855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8074..8178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8246..8264
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8364..8405,1..338)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
       61 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      541 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcttccgata gactgcgtcg
      601 cccgggtacc cgtattccca ataaagcctc ttgctgtttg catccgaatc gtggtctcgc
      661 tgttccttgg gagggtctcc tctgagtgat tgactaccca cgacgggggt ctttcatttg
      721 ggggctcgtc cgggatttgg agacccctgc ccagggacca ccgacccacc accgggaggt
      781 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatgt ttgatgttat
      841 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
      901 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
      961 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
     1021 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1081 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
     1141 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     1201 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     1261 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     1321 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     1381 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     1441 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     1501 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     1561 ctccctttat ccagccctca ctccttctct aggcgccgga attgaagatc tcgatgaggc
     1621 cctttcgtct tcactcgagt ttactcccta tcagtgatag agaacgtatg aagagtttac
     1681 tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta
     1741 taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg
     1801 atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac
     1861 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtgggc gcctataaaa
     1921 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     1981 atacttggat ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc
     2101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2161 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2221 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2461 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac
     2521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2581 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2701 gacgagctgt acaagggtgg cggaggtagt cctggggcct cgaagctccg ggcggttttg
     2761 gagaagttga agctcagccg cgatgatatc tccacggcgg cggggatggt gaaaggggtt
     2821 gtggaccacc tgctgctcag actgaagtgc gactccgcgt tcagaggcgt cgggctgctg
     2881 aacaccggga gctactatga gcacgtgaag atttctgcac ctaatgaatt tgatgtcatg
     2941 tttaaactgg aagtccccag aattcaacta gaagaatatt ccaacactcg tgcatattac
     3001 tttgtgaaat ttaaaagaaa tccgaaagaa aatcctctga gtcagttttt agaaggtgaa
     3061 atattatcag cttctaagat gctgtcaaag tttaggaaaa tcattaagga agaaattaac
     3121 gacattaaag atacagatgt catcatgaag aggaaaagag gagggagccc tgctgtaaca
     3181 cttcttatta gtgaaaaaat atctgtggat ataaccctgg ctttggaatc aaaaagtagc
     3241 tggcctgcta gcacccaaga aggcctgcgc attcaaaact ggctttcagc aaaagttagg
     3301 aagcaactac gactaaagcc attttacctt gtacccaagc atgcaaagga aggaaatggt
     3361 ttccaagaag aaacatggcg gctatccttc tctcacatcg aaaaggaaat tttgaacaat
     3421 catggaaaat ctaaaacgtg ctgtgaaaac aaagaagaga aatgttgcag gaaagattgt
     3481 ttaaaactaa tgaaatacct tttagaacag ctgaaagaaa ggtttaaaga caaaaaacat
     3541 ctggataaat tctcttctta tcatgtgaaa actgccttct ttcacgtatg tacccagaac
     3601 cctcaagaca gtcagtggga ccgcaaagac ctgggcctct gctttgataa ctgcgtgaca
     3661 tactttcttc agtgcctcag gacagaaaaa cttgagaatt attttattcc tgaattcaat
     3721 ctattctcta gcaacttaat tgacaaaaga agtaaagaat ttctgacaaa gcaaattgaa
     3781 tatgaaagaa acaatgagtt tccagttttt gatgaatttt gagcggccgc gccggcgggc
     3841 ccgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3901 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3961 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     4021 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     4081 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     4141 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     4201 ggctgggaag gggtgggtcc gggggngggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4261 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4321 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     4381 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4441 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4681 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4741 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4921 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     4981 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata tcagtggtcc
     5041 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     5101 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5161 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     5221 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc
     5281 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     5341 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     5401 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     5461 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     5521 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     5581 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc
     5641 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5701 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5761 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc
     5821 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5881 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6001 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6061 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6121 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta
     6181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6241 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7501 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     7561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7801 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     7861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8221 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     8281 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     8341 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
     8401 tacgg
//
