LOCUS       sequence-366163-       10416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human KIF1C with mCherry and FRB tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A
  TITLE     Force generation of KIF1C is impaired by pathogenic mutations.
  JOURNAL   Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 
            10.1016/j.cub.2022.07.029. Epub 2022 Aug 11.
  PUBMED    35961316
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.07.029"; journalName: "Curr Biol"; date: 
            "2022-09-12- 12"; volume: "32"; issue: "17"; pages: "3862-3870.e6"
COMMENT     Sequence Label: pKIF1C-mCherry-FRB
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     559..581
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2901..2910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3993..4002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3999..4706
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4144..4162)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4412..4431
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4713..4991
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    5190..5324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5227..5246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5281..5300
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5338..5354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5338..5354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5351..5373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5362..5378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5386..5392,5393..5410,5411..5416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5431..5452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5569..5586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5740..6328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5820..5839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6499..7290,7291..7359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7122..7141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7360..7464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7532..7550
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7588..7610)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7710..7729
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7923..7945
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7937..7954
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7938..7954
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             9871..9906
                     /estimated_length=36
ORIGIN
        1 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
       61 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      121 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      181 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      241 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      301 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
      361 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
      421 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
      481 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
      541 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
      601 cttctctgtt cctccgcagc ccccaagcta gcgctaccgg actcagatct cgagctcaag
      661 cttcgaattc tggagctatg gctggtgcct cggtgaaagt ggcagtgagg gttcggccct
      721 ttaacgcccg tgagaccagc caggatgcca agtgtgtggt cagcatgcag ggcaacacca
      781 cctccatcat caatcctaaa cagagcaagg atgcccccaa aagcttcacc tttgactact
      841 cctactggtc acacacttcg acggaggacc cccagtttgc atctcagcag caagtgtatc
      901 gggacatcgg agaagagatg ctgctccacg cctttgaagg ctacaacgtg tgcatctttg
      961 cctatgggca gaccggggct gggaaatcct ataccatgat ggggcgacag gagccagggc
     1021 agcagggcat cgtgccccag ctctgtgagg acctcttctc tcgcgttagt gagaaccaga
     1081 gtgctcagct atcctactct gtggaggtgt cttacatgga aatctactgt gagcgggtac
     1141 gagacctctt gaaccccaag agtcggggtt ctctgcgggt ccgggagcac cccatcctgg
     1201 gcccgtacgt gcaggacctg tccaaattgg ctgtgacctc ctacgcagac attgctgacc
     1261 tcatggactg tggaaataaa gcacggactg tggctgccac caacatgaat gagaccagca
     1321 gccgttccca tgccgtcttt accatcgtct tcacacagcg ctgccatgac cagctcacgg
     1381 ggctggactc ggagaaggtc agtaagatca gtttggtgga ccttgctggg agtgagcgag
     1441 ccgactcctc aggggcccgg ggcatgcgcc tgaaggaagg agccaacatc aataagtccc
     1501 tgactacact agggaaagtg atctcggccc ttgcagatat gcaatcaaag aagcgaaagt
     1561 cggattttat cccctacagg gactctgtgc tcacctggct gctcaaggaa aatttggggg
     1621 ggaactcacg cacagccatg attgcagccc tgagccctgc tgacatcaat tacgaggaga
     1681 ctctcagcac cctcaggtat gctgaccgca ccaagcaaat ccgctgcaat gccatcatca
     1741 acgaggaccc taatgcccgg ctgattagag agctgcagga ggaagtagcc cggctgcggg
     1801 aactgctgat ggctcaggga ctgtcagcct ctgctctgga aggcctgaag acggaagaag
     1861 ggagtgtcag aggcgccctg ccagctgtgt catctccccc agctccagtt tcaccctcat
     1921 cacccaccac acataatggg gagctggagc cgtcattctc ccccaacacg gagtcccaga
     1981 ttgggcctga ggaagccatg gagaggctgc aggagacaga gaagattata gctgagctga
     2041 acgagacatg ggaggagaag ctacgcaaga cagaagccct gaggatggag agagaagcat
     2101 tgctggctga gatgggggtg gccgtccggg aggatggggg aactgtgggc gtcttctctc
     2161 caaagaagac tccccacctg gtgaacctga acgaagaccc tctgatgtct gagtgtctgc
     2221 tctaccacat caaagatggc gtcaccaggg tcggccaagt agatatggac atcaagctga
     2281 ccggacagtt cattcgggag caacactgtc tgttccggag catcccccag ccagatggag
     2341 aagtggtggt cactctggag ccttgtgaag gagctgagac atatgtgaat gggaagcttg
     2401 tgacggagcc gctggtgctg aagtcaggga ataggattgt gatgggcaag aaccacgttt
     2461 tccgcttcaa ccacccggag caggcaaggc tggaacggga acgaggggtc cccccacccc
     2521 caggaccgcc ctctgagcca gtcgactgga actttgccca gaaggaactg ctggagcagc
     2581 aaggcatcga cataaagctg gaaatggaga agaggctgca ggatctggag aatcagtacc
     2641 ggaaagaaaa ggaagaagcc gatcttctgc tggagcagca gcgactgtat gcagactcgg
     2701 acagcgggga tgactctgac aagcgctctt gtgaagagag ctggaggctc atctcctcct
     2761 tgcgggagca gctgccgccc accacggtcc agaccattgt caaacgctgt ggtctgccca
     2821 gcagtggcaa gcgcagggcc cctcgcaggg tttatcagat cccccagcga cgcaggctgc
     2881 agggcaaaga cccccgctgg gccaccatgg ctgacctgaa gatgcaggcg gtgaaggaga
     2941 tctgctacga ggtggccctg gctgacttcc gccacgggcg ggctgagatt gaggccctgg
     3001 ccgccctcaa gatgcgggag ctgtgtcgca cctatggcaa gccagacggc cccggagacg
     3061 cctggagggc tgtggcccgg gatgtctggg acactgtagg cgaggaggaa ggaggtggag
     3121 ctggcagtgg tggtggcagt gaggagggag cccgaggggc ggaggtggag gacctccggg
     3181 cccacatcga caagctgacg gggattctgc aggaggtgaa gctgcagaac agcagcaagg
     3241 accgggagct gcaggccctg cgggaccgca tgctccgcat ggagagggtc atccccctgg
     3301 cccaggatca tgaggatgag aatgaagaag gtggtgaggt cccctgggcc ccgcctgaag
     3361 gatcagaggc agcagaggag gcagccccca gtgaccgcat gccgtcagcc cggcccccct
     3421 cgccaccact gtcaagctgg gagcgggtgt cacggctcat ggaggaggac cctgccttcc
     3481 gtcgtggtcg tcttcgctgg ctcaagcagg agcagctacg gctgcaggga ctgcagggct
     3541 ctgggggccg gggcgggggg ctgcgcaggc ccccagcccg ctttgtgccc cctcacgact
     3601 gcaagctacg cttccccttc aagagcaacc cccagcaccg ggagtcttgg ccagggatgg
     3661 ggagcgggga ggctccaact ccgctccaac cccctgagga ggtcactccc catccagcca
     3721 cccctgcccg ccggcctccg agtccccgaa ggtcccacca tccccgcagg aactccctgg
     3781 atggaggggg ccgatcccgg ggagcgggtt ctgcacagcc tgaaccccag cacttccagc
     3841 ccaaaaagca caactcttat ccccagccac cccaacccta cccagcccag cggcccccag
     3901 ggccccgcta ccccccatac actactcccc cacgaatgag acggcagcgt tctgcccctg
     3961 acctcaagga gagtggggca gctgtggatc ccgccaccat ggtgagcaag ggcgaggagg
     4021 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     4081 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4141 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     4201 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     4261 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4321 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     4381 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     4441 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     4501 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     4561 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     4621 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     4681 ccggcggcat ggacgagctg tacaagacta gtatcctctg gcatgagatg tggcatgaag
     4741 gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg
     4801 tgctggagcc cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct
     4861 ttaatcaggc ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga
     4921 aatcagggaa tgtcaaggac ctcctccaag cctgggacct ctattatcat gtgttccgac
     4981 gaatctcaaa gtaagcggcc gccatatgca tcctaggcct attaatattc cggagtatac
     5041 gtagccggct aacgttaaca accggtacct ctagataagt aatgatcata atcagccata
     5101 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5161 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5221 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5281 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcg taatcatgtc
     5341 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5401 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5461 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5521 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6181 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6601 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7501 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     7561 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     7621 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7681 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7741 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     7801 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     7861 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     7921 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt tgcattggcc
     7981 cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg aaactgatgg
     8041 tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca gcacacagtg
     8101 gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa tcaagggaac
     8161 ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca caccccacgc
     8221 tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg cggggcattt
     8281 cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc ctcgtggctg
     8341 cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg gagcacaaac
     8401 gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt gctgcgagtg
     8461 gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac tgggtgccca
     8521 cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg tccctgcggt
     8581 gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga cacaatgcag
     8641 ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc aagaccgcac
     8701 acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc ccttaatcaa
     8761 tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct
     8821 gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag
     8881 aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc
     8941 gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc
     9001 cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg
     9061 cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt
     9121 gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc
     9181 gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg
     9241 cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttgatggtg
     9301 cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat acccctcggt
     9361 gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg catgcactca
     9421 cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag ctcagtggct
     9481 gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct
     9541 cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg
     9601 aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg
     9661 accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga
     9721 ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc
     9781 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     9841 ttttgtgcag cgatgggggc gggggggggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnngggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     9961 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
    10021 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
    10081 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
    10141 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg
    10201 tggctgcgtg aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc
    10261 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
    10321 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
    10381 gagcgcggcc gggggcggtg ccccgcggtg cggggg
//
