LOCUS       sequence-36618-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0037
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gngaattccc tctanaatat tttgtttaac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggcctctgg
      121 agccgattca aaaggtgatg atttatcaac agccattctc aaacagaaga accgacccaa
      181 tcggttaatt gttgatgaag ccatcaatga agataacagc gtggtgtcct tgtcccagcc
      241 caagatggat gaactgcagt tgttccgagg tgacacggtg ttgctaaaag gaaagaaaag
      301 acgggaagct gtatgcattg ttctttctga tgacacgtgt tctgatgaga agattcgaat
      361 gaatagagtt gttcggaata acctccgagt tcgcctagga gatgtcatca gcatccagcc
      421 atgccctgat gtaaagtatg gcaaacgtat ccacgttcta cccatcgatg acacagtgga
      481 aggcatcact ggcaatctct ttgaggtata ccttaagccg tacttcctgg aagcttatcg
      541 gcccatccgt aaaggagata tttttcttgt ccggggtggg atgcgtgctg tggagttcaa
      601 agttgtagag acagatccca gcccttactg tattgttgct ccagacacag tgatccactg
      661 tgagggggag ccaatcaaac gagaggatga ggaggaatcc ttgaatgaag taggctatga
      721 tgacgtcggt ggttgcagga agcagctagc tcagataaag gagatggtgg agctgccact
      781 gagacatcct gcgctcttca aggcgattgg tgtaaagcct cctcggggaa tcttgttgta
      841 tgggcctcct gggacaggga agaccctgat tgctcgagct gtggcaaatg aaactggagc
      901 cttcttcttt ctgatcaatg gtcctgaaat catgagcaaa ttggctggtg agtctgagag
      961 cacctncgta agct
//
