LOCUS       sequence-366182-       10405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2Gi2R assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, 
            McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
            Winkler J, Yao J, Hetzer MW, Gage FH
  TITLE     Directly Reprogrammed Human Neurons Retain Aging-Associated 
            Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic 
            Defects.
  JOURNAL   Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001.
  PUBMED    26456686
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001."
COMMENT     Sequence Label: pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2283..3461
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2514..3452
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3409..3429
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3489..3491,3492..3494,3495..4205)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3522..3550)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4145..4165
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    4227..4251
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4260..4286
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(4296..4298,4299..4301,4302..5015)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4296..4298,4299..4301,4302..5012)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4341..4362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4602..4621)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4949..4970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5024..5597
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5191..5208)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5418..5437
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5613..5633
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5634..6338
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5776..5794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6044..6063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    6366..6390
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6393..7082
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     LTR             7169..7802
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7930..7946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7930..7946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7943..7965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7954..7970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7978..7984,7985..8002,8003..8008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8023..8044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8161..8178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8332..8920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8412..8431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9091..9882,9883..9951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9714..9733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9952..10056)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10104..10238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10141..10160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10195..10214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgcgccc gacgataagc tttgcaaaga tggataaagt
     2221 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     2281 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2341 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2521 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2581 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2641 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2701 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2761 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2821 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2881 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2941 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     3001 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3061 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3121 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3181 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3241 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3301 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3361 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3421 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaatttcg acccggatcc
     3481 gcggccgcat ggccagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     3541 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     3601 ctacatacgg aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     3661 ggccaacact tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc
     3721 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca
     3781 ctatatcttt caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg
     3841 atacccttgt taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     3901 tcggacacaa actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac
     3961 aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc
     4021 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     4081 acaaccatta cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc
     4141 acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct
     4201 acaaaagcgg ttccggaccg gatcaaacaa gtttgtacaa aaaagcaggc ttactacagc
     4261 taccgccgct ggaaagactg actctgggag gcgggatggt gagcaagggc gaggagctgt
     4321 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4381 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4441 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4501 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4561 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4621 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4681 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4741 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     4801 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4861 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4921 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4981 ggatcactct cggcatggac gagctgtaca agtaggaatt ccgcccctct ccctcccccc
     5041 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5101 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5161 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5221 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5281 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5341 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5401 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5461 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5521 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5581 gaaaaacacg atgataagct tgccgtacga tgccaaaaaa gaaaagaaaa gttgtgagca
     5641 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5701 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5761 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5821 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5881 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5941 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6001 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6061 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6121 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6181 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6301 gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     6361 acatcacaag tttgtacaaa aaagcaggct taatgagcga gctgattaag gagaacatgc
     6421 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg
     6481 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     6541 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc agaaccttca
     6601 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat
     6661 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
     6721 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg
     6781 gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg ctgtaccccg
     6841 ctgacggcgg cctggaaggc agaagcgaca tggccctgaa gctcgtgggc gggggccacc
     6901 tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     6961 ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct
     7021 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     7081 acaaacttaa ttagtagggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7141 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     7201 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     7261 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7321 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     7381 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     7441 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     7501 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     7561 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     7621 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7801 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7861 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     7921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7981 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8041 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8101 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8161 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8221 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10081 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    10141 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10201 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10261 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10321 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10381 aaatatgtac tacaaactta gtagt
//
