LOCUS       sequence-366183-       10118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human KIF1C with R169W mutation and mCherry 
            and FRB tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A
  TITLE     Force generation of KIF1C is impaired by pathogenic mutations.
  JOURNAL   Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 
            10.1016/j.cub.2022.07.029. Epub 2022 Aug 11.
  PUBMED    35961316
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.07.029"; journalName: "Curr Biol"; date: 
            "2022-09-12- 12"; volume: "32"; issue: "17"; pages: "3862-3870.e6"
COMMENT     Sequence Label: pKIF1C_R169W-mCherry-FRB
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     700..722
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3042..3051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4134..4143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4140..4847
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4285..4303)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4553..4572
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4854..5132
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    5331..5465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5368..5387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5422..5441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5479..5495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5479..5495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5492..5514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5503..5519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5527..5533,5534..5551,5552..5557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5572..5593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5710..5727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5881..6469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5961..5980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6640..7431,7432..7500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7263..7282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7501..7605)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7673..7691
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7729..7751)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7851..7870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8064..8086
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8078..8095
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8079..8095
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggggggggg gggggggggg gggggsgkgr rrrrgggggg gggggggggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg ggggggskkg gggsgsksgg
      121 gggggggggg ggggggrgsg sggssggggg sggkgssssg sggkgsgggg ggggckgcga
      181 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
      241 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
      301 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggcgg
      361 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
      421 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
      481 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
      541 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
      601 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct ccagcctcgg ggctgtccgc
      661 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
      721 ccggcggggt ttatatcttc ccttctctgt tcctccgcag cccccaagct agcgctaccg
      781 gactcagatc tcgagctcaa gcttcgaatt ctggagctat ggctggtgcc tcggtgaaag
      841 tggcagtgag ggttcggccc tttaacgccc gtgagaccag ccaggatgcc aagtgtgtgg
      901 tcagcatgca gggcaacacc acctccatca tcaatcctaa acagagcaag gatgccccca
      961 aaagcttcac ctttgactac tcctactggt cacacacttc gacggaggac ccccagtttg
     1021 catctcagca gcaagtgtat cgggacatcg gagaagagat gctgctccac gcctttgaag
     1081 gctacaacgt gtgcatcttt gcctatgggc agaccggggc tgggaaatcc tataccatga
     1141 tggggcgaca ggagccaggg cagcagggca tcgtgcccca gctctgtgag gacctcttct
     1201 ctcgcgttag tgagaaccag agtgctcagc tatcctactc tgtggaggtg tcttacatgg
     1261 aaatctactg tgagcgggta cgagacctct tgaaccccaa gagtcggggt tctctgcggg
     1321 tctgggagca ccccatcctg ggcccgtacg tgcaggacct gtccaaattg gctgtgacct
     1381 cctacgcaga cattgctgac ctcatggact gtggaaataa agcacggact gtggctgcca
     1441 ccaacatgaa tgagaccagc agccgttccc atgccgtctt taccatcgtc ttcacacagc
     1501 gctgccatga ccagctcacg gggctggact cggagaaggt cagtaagatc agtttggtgg
     1561 accttgctgg gagtgagcga gccgactcct caggggcccg gggcatgcgc ctgaaggaag
     1621 gagccaacat caataagtcc ctgactacac tagggaaagt gatctcggcc cttgcagata
     1681 tgcaatcaaa gaagcgaaag tcggatttta tcccctacag ggactctgtg ctcacctggc
     1741 tgctcaagga aaatttgggg gggaactcac gcacagccat gattgcagcc ctgagccctg
     1801 ctgacatcaa ttacgaggag actctcagca ccctcaggta tgctgaccgc accaagcaaa
     1861 tccgctgcaa tgccatcatc aacgaggacc ctaatgcccg gctgattaga gagctgcagg
     1921 aggaagtagc ccggctgcgg gaactgctga tggctcaggg actgtcagcc tctgctctgg
     1981 aaggcctgaa gacggaagaa gggagtgtca gaggcgccct gccagctgtg tcatctcccc
     2041 cagctccagt ttcaccctca tcacccacca cacataatgg ggagctggag ccgtcattct
     2101 cccccaacac ggagtcccag attgggcctg aggaagccat ggagaggctg caggagacag
     2161 agaagattat agctgagctg aacgagacat gggaggagaa gctacgcaag acagaagccc
     2221 tgaggatgga gagagaagca ttgctggctg agatgggggt ggccgtccgg gaggatgggg
     2281 gaactgtggg cgtcttctct ccaaagaaga ctccccacct ggtgaacctg aacgaagacc
     2341 ctctgatgtc tgagtgtctg ctctaccaca tcaaagatgg cgtcaccagg gtcggccaag
     2401 tagatatgga catcaagctg accggacagt tcattcggga gcaacactgt ctgttccgga
     2461 gcatccccca gccagatgga gaagtggtgg tcactctgga gccttgtgaa ggagctgaga
     2521 catatgtgaa tgggaagctt gtgacggagc cgctggtgct gaagtcaggg aataggattg
     2581 tgatgggcaa gaaccacgtt ttccgcttca accacccgga gcaggcaagg ctggaacggg
     2641 aacgaggggt ccccccaccc ccaggaccgc cctctgagcc agtcgactgg aactttgccc
     2701 agaaggaact gctggagcag caaggcatcg acataaagct ggaaatggag aagaggctgc
     2761 aggatctgga gaatcagtac cggaaagaaa aggaagaagc cgatcttctg ctggagcagc
     2821 agcgactgta tgcagactcg gacagcgggg atgactctga caagcgctct tgtgaagaga
     2881 gctggaggct catctcctcc ttgcgggagc agctgccgcc caccacggtc cagaccattg
     2941 tcaaacgctg tggtctgccc agcagtggca agcgcagggc ccctcgcagg gtttatcaga
     3001 tcccccagcg acgcaggctg cagggcaaag acccccgctg ggccaccatg gctgacctga
     3061 agatgcaggc ggtgaaggag atctgctacg aggtggccct ggctgacttc cgccacgggc
     3121 gggctgagat tgaggccctg gccgccctca agatgcggga gctgtgtcgc acctatggca
     3181 agccagacgg ccccggagac gcctggaggg ctgtggcccg ggatgtctgg gacactgtag
     3241 gcgaggagga aggaggtgga gctggcagtg gtggtggcag tgaggaggga gcccgagggg
     3301 cggaggtgga ggacctccgg gcccacatcg acaagctgac ggggattctg caggaggtga
     3361 agctgcagaa cagcagcaag gaccgggagc tgcaggccct gcgggaccgc atgctccgca
     3421 tggagagggt catccccctg gcccaggatc atgaggatga gaatgaagaa ggtggtgagg
     3481 tcccctgggc cccgcctgaa ggatcagagg cagcagagga ggcagccccc agtgaccgca
     3541 tgccgtcagc ccggcccccc tcgccaccac tgtcaagctg ggagcgggtg tcacggctca
     3601 tggaggagga ccctgccttc cgtcgtggtc gtcttcgctg gctcaagcag gagcagctac
     3661 ggctgcaggg actgcagggc tctgggggcc ggggcggggg gctgcgcagg cccccagccc
     3721 gctttgtgcc ccctcacgac tgcaagctac gcttcccctt caagagcaac ccccagcacc
     3781 gggagtcttg gccagggatg gggagcgggg aggctccaac tccgctccaa ccccctgagg
     3841 aggtcactcc ccatccagcc acccctgccc gccggcctcc gagtccccga aggtcccacc
     3901 atccccgcag gaactccctg gatggagggg gccgatcccg gggagcgggt tctgcacagc
     3961 ctgaacccca gcacttccag cccaaaaagc acaactctta tccccagcca ccccaaccct
     4021 acccagccca gcggccccca gggccccgct accccccata cactactccc ccacgaatga
     4081 gacggcagcg ttctgcccct gacctcaagg agagtggggc agctgtggat cccgccacca
     4141 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     4201 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     4261 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     4321 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     4381 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     4441 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     4501 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     4561 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     4621 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     4681 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     4741 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     4801 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagact agtatcctct
     4861 ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt ggggaaagga
     4921 acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa cggggccccc
     4981 agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg gaggcccaag
     5041 agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa gcctgggacc
     5101 tctattatca tgtgttccga cgaatctcaa agtaagcggc cgccatatgc atcctaggcc
     5161 tattaatatt ccggagtata cgtagccggc taacgttaac aaccggtacc tctagataag
     5221 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
     5281 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5461 ggatcaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7621 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7681 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7741 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7801 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     7861 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7921 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     7981 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     8041 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     8101 gttgggatct ttgcattggc ccacggctct caggatgggg atgctcccct tcagcacccg
     8161 gttccccttg gaaactgatg gtcctggctc tgtggcatgg cagtggcact gtgaggagcc
     8221 ccctaccagc agcacacagt ggggttggca ctgccacgct ccggatgccg cgctctgatc
     8281 caaccccata atcaagggaa cccgaattgc cccatcattg cccccaccac ccccatcctg
     8341 ccgggccctc acaccccacg ctgccttctg gtgacattcc ccagcccaaa cccacggctt
     8401 catggctacc gcggggcatt tcccattgcc gccccattat cagctctgca cacctcccgc
     8461 tgtacccatg cctcgtggct gcccttcttt gacgtataat cttctaatta atacccggcc
     8521 ttgtcaaagt ggagcacaaa cgttaattaa ttccccagca ggcaggtaat taacagtgtg
     8581 actccctttt tgctgcgagt ggggctgata cagagagatg tggcactatg gagcccacgg
     8641 ggtcctggca ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg
     8701 agacagtatt gtccctgcgg tgtccctgca gctcagctct gtccacaggg ccacctccag
     8761 tttggagggg acacaatgca gccccgatgc aacccatcct cgcagcatcc cagggacaaa
     8821 gaccccactg caagaccgca cacagggctg ggtcccgctc ccctaatatc tacagtgctt
     8881 cttgcatggc cccttaatca atgcagttaa tcagcatgcg ctcatgcacc gctctggagc
     8941 tgcaaagccc ctcgcagcgc tgctcaccaa caccgcgcac cgccccggcc cagcctgcag
     9001 cacgcgctgc aaacaggaaa gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc
     9061 tgccttgacc tccgccgggc cgggccctgc ctgactcagc tccttactca gcgctcgctt
     9121 cctccctccg gctgccaccg ccgcagcgca caccctgaca aagagtggcc cttaacgggc
     9181 tctgaggtgc acccagcagt gcactcagca gtccaagggc cggcctggag gtttgcaccg
     9241 ctacgtgctg acattagcat tgaacttggc cctgggtagt gctgcaggcc gggcggggtg
     9301 ggtgtagaga gtgcagcgcg cgttgcaccc ggtgcccctt cccctccctt gcatcccagc
     9361 aggctgcacc ccagcaccag gcccgtgcat gcatgctcct ggtgttattg cagcctggtg
     9421 catgcatgcg tcttgatggt gcagcgctgt gcatgcatcc tccttggtgt gtagcagtct
     9481 agtgcatgca tacccctcgg tgttattgct gctctgtgca cgcacgctca ttgtatcact
     9541 tcatcccagt gcatgcactc acactggagc gattgctgct cggtgcacgc acactcattg
     9601 tatcactgca gctcagtggc tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg
     9661 cacatcagtg tcgctgcagc tcagtgcatg cacgctcatt gcccatcgct atccctgcct
     9721 ctcctgctgg cgctccccgg gaggtgactt caaggggacc gcaggaccac ctcgggggtg
     9781 gggggagggc tgcacacgcg gaccccgctc cccctcccca acaaagcact gtggaatcaa
     9841 aaagggggga ggggggatgg aggggcgcgt cacacccccg ccccacaccc tcacctcgag
     9901 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     9961 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggcgcgc
    10021 gccaggcggg gsgggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    10081 gggcmarmar rggggggggg cccsgrrggt tkyktttt
//
