LOCUS       sequence-36620-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0028
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaattccnct naatattttg tttactttag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcct ctggagccga
      121 ttcaaaaggt gatgatttat caacagccat tctcaaacag aagaaccgac ccaatcggtt
      181 aattgttgat gaagccatca atgaagataa cagcgtggtg tccttgtccc agcccaagat
      241 ggatgaactg cagttgttcc gaggtgacac ggtgttgcta aaaggaaaga aaagacggga
      301 agctgtatgc attgttcttt ctgatgacac gtgttctgat gagaagattc gaatgaatag
      361 agttgttcgg aataacctcc gagttcgcct aggagatgtc atcagcatcc agccatgccc
      421 tgatgtaaag tatggcaaac gtatccacgt tctacccatc gatgacacag tggaaggcat
      481 cactggcaat ctctttgagg tataccttaa gccgtacttc ctggaagctt atcggcccat
      541 ccgtaaagga gatatttttc ttgtccgggg tgggatgcgt gctgtggagt tcaaagttgt
      601 agagacagat cccagccctt actgtattgt tgctccagac acagtgatcc actgtgaggg
      661 ggagccaatc aaacgagagg atgaggagga atccttgaat gaagtaggct atgatgacgt
      721 cggtggttgc aggaagcagc tagctcagat aaaggagatg gtggagctgc cactgagaca
      781 tcctgcgctc ttcaaggcga ttggtgtaaa gcctcctcgg ggaatcttgt tgtatgggcc
      841 tcctgggaca gggaagaccc tgattgctcg agctgtggca aatgaaactg gagccttctt
      901 ctttctgatc aatggtcctg aaatcatgag caattgctgt gatngaacac tctagctnga
//
