LOCUS       sequence-36621-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0050
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tggaattccc nctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gcctctggag
      121 ccgattcaaa aggtgatgat ttatcaacag ccattctcaa acagaagaac cgacccaatc
      181 ggttaattgt tgatgaagcc atcaatgaag ataacagcgt ggtgtccttg tcccagccca
      241 agatggatga actgcagttg ttccgaggtg acacggtgtt gctaaaagga aagaaaagac
      301 gggaagctgt atgcattgtt ctttctgatg acacgtgttc tgatgagaag attcgaatga
      361 atagagttgt tcggaataac ctccgagttc gcctaggaga tgtcatcagc atccagccat
      421 gccctgatgt aaagtatggc aaacgtatcc acgttctacc catcgatgac acagtggaag
      481 gcatcactgg caatctcttt gaggtatacc ttaagccgta cttcctggaa gcttatcggc
      541 ccatccgtaa aggagatatt tttcttgtcc ggggtgggat gcgtgctgtg gagttcaaag
      601 ttgtagagac agatcccagc ccttactgta ttgttgctcc agacacagtg atccactgtg
      661 agggggagcc aatcaaacga gaggatgagg aggaatcctt gaatgaagta ggctatgatg
      721 acgtcggtgg ttgcaggaag cagctagctc agataaagga gatggtggag ctgccactga
      781 gacatcctgc gctcttcaag gcgattggtg taaagcctcc tcggggaatc ttgttgtatg
      841 ggcctcctgg gacagggaag accctgattg ctcgagctgt ggcaaatgaa actggagcct
      901 tcttctttct gatcaatggt cctgaaatca tgagcaattg gctggtgagt ctgaaagcaa
      961 ccttctntag ctagacaaag cgt
//
