LOCUS       sequence-366232-       12543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express Flag-GluN2A from a 
            1.3CaMKII promoter. Transgene in reverse orientation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP209-Lenti-1.3CaMKII P-Intron-Flag-GluN2A-W...
FEATURES             Location/Qualifiers
     source          1..12543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2055..2643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2161..2172
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2570..2590
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6888..6911)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7070..7079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          7143..7618
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     promoter        complement(7698..8986)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     LTR             9064..9297
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9369..9490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9460..9479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9530..9665
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9685..9704)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9686..9704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9714..9731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9714..9730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9723..9745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9872..10327
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9959..9978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10169..10190
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10353..10457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10458..10526,10527..11318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10676..10695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11489..12077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11978..11997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12231..12248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12365..12386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12401..12406,12407..12424,12425..12431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12439..12455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12444..12466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12463..12479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12463..12479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12498..12518
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12500..12518
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtcaattgg cggccgcctc gacagtgaaa aaaatgcttt atttgtgaaa
     1981 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaactc gagccacacc
     2041 ggtaccatcg ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     2101 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     2161 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     2221 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     2281 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     2341 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     2401 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     2461 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     2521 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     2581 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     2641 attgtcgact cgagttaaac atcagattcg atactaggca ttttcttgta cactcgtcta
     2701 ttgctgcagg aatttaaaac cctgggggta gagtacatgg tattcttatt tgcagcataa
     2761 ggcataacat gctctgaaat atacacatca ctgtggcccc gactgtccct ggagcaatat
     2821 gatgctgttg acctcaagga tgaccgaaga tagctgtcat ttaccgcctg tgatggcaat
     2881 gagtgtttgt aagggtccga gggacatctc ccgataacta agcgttggtc atccccatac
     2941 gtgtggagga aaggattatc agaggcgtgg tctggcaaga gagacttgct cctcttgctg
     3001 tcctccagac cttgggggaa aagggagctt ttgttcccca agagtttgct tgaggggaca
     3061 ctgaacaggc tcccatacaa gttgccctcc agtagccgtt ccctgtcctt gaggcttatg
     3121 ctccgggagg gcctgctaag gtctatctct ctgggtttgt ccagaatgtt atcataggag
     3181 tgctgtcggt taatccttag cttgttcttc tggaactgga gggcgttgtt ctgtgaccag
     3241 tcctgctggt agacctcctc ccgagtagct gggttacctg tctcctgaag catctggtct
     3301 tcatcaatgt catagagatt ccccatccgc agacaggcat cacacttgaa aggagacctc
     3361 atggtaaagt ggcctgagta ggtgggcaga ttcgaaaggc agcttctgca atgtgtggag
     3421 ttctgccggt atcgatcact gccctcactg tgtggggaac ccttgtcttt caaggtaaag
     3481 tgcttggcat agagtttata ttggtcattg ttgggaagcc cgtcttcatt atgtaatggg
     3541 ttcctgttca tgggcagtgt ggagtccccc ttgcggaagt tctcattgtg atcttggtag
     3601 gtatctggga agcccacatt ctcaggcagg gttatattct caacaaactg aggaggatct
     3661 aagtggaagc tgggctcctt ctcaccatca atggtataga tcttatccct gggagaactt
     3721 gctttggttt tcatgtaggt gcggtcaaca tcgctacagt ccttgggata cttagaggcc
     3781 attgaccgtt tgaagttatc cttggtcttg tggttcttat tgttatctgg ctccctgtgg
     3841 catgtggccc ggcttgaggt ttctgaaatg tcagagtggg ctacctcttc tggaagatac
     3901 ctaggactct ttagcgagtg ggtccgattc tctgcagtct tctcatccct ctgggagact
     3961 gggttctggt ttagagaatc ctggcgtaga gactccatgg atttcttcca aagctgccgg
     4021 ggtcgggagt tccctttgga ttcagtgctg acagccacct ctactgtgtt agggttggac
     4081 tcattgagag tgagaggatg ctgtccttga aacacatagt tactgagatt atccttgtgc
     4141 ctgttggcca caaatgtttg gagttcattc atgttgtctc caaatatact gtccttccct
     4201 tgaaaggatc tgttgtctga atatatcaaa tttcccttgt ctgaaaccat gtccacaata
     4261 agtgaccctc tttgaatgaa gtcagtagct cttttgggtg agtccattct tgaggagttc
     4321 atgttggaca tattggagat gtttttagct gaccgaagaa gctttagcat gttgctctgt
     4381 gatccagtca gattgaaatc tggagatttc ttcttttctt caatgtgtac cccatggatg
     4441 caactataga tgcccctgct gatggagaag agcagcccgg gccggtcaga gcacacgcct
     4501 gtgaagcaga agcgcagctt ccagtagaag aggtgctccc agatgaaggt gatgaggctg
     4561 agggccatgg ctgcagccag catgtagaac acgcccgcca tgttatcgat gtccagctgg
     4621 ctactcatca cctcattctt ctcgttgtgg cagatgcccg taagccacag tgtctccagc
     4681 tcctccatct caccatcacc aacaaactgg agcagagcga ggtcaatctg cctcttccag
     4741 ggtgagccct tctgcagcgc aattccatag cctgtggtag caaagatgta cccgctccca
     4801 atggtcacca gtttacagcc ttcatccctc ccggccttgt agttcaagac ggctgcgtca
     4861 tagatgaaag cgtccaactt cccggttttc aagctgacca aggcatcctc cactcccctc
     4921 tggttgaatc tggtcatgta ctggtgcata taggggtagt tgttacgaat attcctctct
     4981 gtacttccat tgggtaccgt cccaaatcgg aaaggtggag aatagtcatg aggtctctgg
     5041 aacttcttgt cactgaggcc agtcacttgg tccacaaact cctcctggat catgaaggca
     5101 gccaggttgg ctgtgtaact ggccaggaag atgacagcaa agaaggccca cactgacacc
     5161 atgatcttgc tggttgtgcc tttaggattc tggacaggca cagaattgtt gaagaccagg
     5221 ccccagagga gccatatagc ttttccaata gtaaaagaag gcccgtgggg agctttccct
     5281 ttggctaagt ttctgttgta tccaacagga ctgaagtatt caaaaacgaa gacagcaatg
     5341 gctgagacga tgagcagcat cacaaacatc atcacccaga cggaggcact gaagggttcg
     5401 aggaaagcag aaggggagac agtgccatta ctcctggaga ccatgacgct gattcctgtc
     5461 tccacgaagg gcaccgagaa gtccaccact tccgaacgct cctcattgat ggtgagtgag
     5521 cccacagcca tgactgctcg ttgatagacc acttcaccta tcattccatt ccacacattg
     5581 ttaacctttt tcccatgctt cccattggtc accaggtaga ggtcataggt gaacttcaca
     5641 gttctggaca gcttcttgag gatgtcgatg cagaacccct tgcagcattt cttcacattc
     5701 atcccttcgt tggttgaatt gttgatcttg acgaacttcc gacagggcac cgtgttcctc
     5761 acacaggtct cagtcagggg gtctatgtcc tctacgatga cgaagggggc ttcctccaag
     5821 gtgacaatgc tgaggtggtt gtcatctggc tcgcagtcag aaaaggactt gtaccttggc
     5881 cacacagcgt gcctcaggct cagcgtctgg ttctcccact tgcccacctt ttcccactcc
     5941 cggtccttgt tcagcacgat caccacaagc ctggggtgca cctggtaacc ttcctcagtg
     6001 aaggacaagt ccttgccatc ccaagtcaca ttgaccatga attggtgcag ggtgtgtagc
     6061 ggggtctctg gcttctctgc ctgcccatag cagctggcct tggcctcagg aatgtaggag
     6121 aacttctcca acatggagga tgcggcagtg gttaagatcc caagaccgtc tctcactctt
     6181 gcctccaggc tgtagtccca gtcgtcataa gagactgaaa tgagacctga tggaaactct
     6241 ttggggatga gctctgtgtt cccagacacc aaactgggga caatccagaa gaaatcatag
     6301 ccagtgaggc cgagggagcg agcctcgctc aggatgagga cagcctcgtc tttggagcag
     6361 tagagcagga tgacagaaga atggatcttc ttcagctgga cctgcgtctt ggcgtcctcg
     6421 aaggaggtgt ccagtgtgat cacgttctgc atatcccagc ccacaaagct gttgtccact
     6481 gttgtcttga taaagctgat gaagtctcgg tagccaggga agatggtggt gaccagggag
     6541 aagacgtgcc agtcgtagtc ctgcatgatc ttcagcataa ctgtggcttg ctgctggatg
     6601 gaggctccaa actggaagaa cgtggatgtc ggatccttgt cagccatgat catagatgca
     6661 cccccatgaa tgcccaagat ggggataaaa gtctgtgagg agataaaatc cagcatctgg
     6721 gccacagcct cctggtccgt gtcatctcca aacaccaagc cgtggatgcg cgccccggac
     6781 atgaggtcgc acacatgcgt gatgaggctc ttagggtcag tgcggttcat caataacgcc
     6841 accacgttca catccagggg caagccggtt gcctgctctg ggccccactt gtcatcgtcg
     6901 tccttgtagt ccagatttcg aagttcgcgt tctgtcacgt cgtggctgtg acccagcagc
     6961 accgcaatgt tcagcgctgg aggacccttc tccgccgccg cgttctgcgc cggatcgcgc
     7021 cagaccagaa gggccggcaa taccagcaag gtccagtagc ccaatctgcc catggtggcg
     7081 gcgaattcac gtgctttgcc aaagtgatgg gccagcacac agaccagcac gttgcccagg
     7141 agctgtggga ggaagataag aggtatgaac atgattagca aaagggccta gcttggactc
     7201 agaataatcc agccttatcc caaccataaa ataaaagcag aatggtagct ggattgtagc
     7261 tgctattagc aatatgaaac ctcttacatc agttacaatt tatatgcaga aatatttata
     7321 tgcagaaata ttgctattgc cttaacccag aaattatcac tgttattctt tagaatggtg
     7381 caaagaggca tgatacattg tatcattatt gccctgaaag aaagagatta gggaaagtat
     7441 tagaaataag ataaacaaaa aagtatatta aaagaagaaa gcatttttta aaattacaaa
     7501 tgcaaaatta ccctgatttg gtcaatatgt gtaccctgtt acttctcccc ttcctatgac
     7561 atgaacttaa ccatagaaaa gaaggggaaa gaaaacatca agggtcccat agactcaccc
     7621 tgaagttctc aggatccgag ctcggcgcgc cgctagcccg cggtcgcgaa cgcgtggccg
     7681 gccgtagggg atcccccgct gcccccagaa ctaggggcca ctcgcctgcc cgtgctcctg
     7741 agtgcaaacg gagaaccggc ttgactgacg agcttggggc ttctgagcag ggcactgtgg
     7801 ctgctcacag cctcgcgagc cttcgtcagc atccaggtcc ccatggcaac cacctccgaa
     7861 acgccctgtt cccgttgccc cggtaactgc ctccccaaca cctgcctgcc tttcacttta
     7921 aaacctgctc ctctttgccc tggccttata tacacaatac tgcaggactg tgtgggccca
     7981 ggagcaagtg gaccctgttc ccccagggtg atgtactgag ggggttggag aaggggatgc
     8041 gaacaaactt agtccacaag tgatcatcgc tcttcctacc tcccccacct ccaactatag
     8101 ggccctttta acagacggga tgggattaag tggggcaaag aggcagttgc tatggtaacg
     8161 gctagtgggt gccacattct gggatggtcc ttgagaaaga caggatgtag ttaccctggc
     8221 aacttcatct cctctgaaga gacaggtatt gccccgttgc cctggcaaca gctgggttct
     8281 aaggtttaca ggggaagcta attcatctct ccttcccttc ccccttgtag tctctgtaat
     8341 ggtttctttg cttcctctgg gaattggtct ggttgacttg gcctctgtct ctgtccttgt
     8401 ttctttccag ctctccctgc tttttagcca ttgtgcatat cacaccgcca ccttagcgtg
     8461 agaagaagta ccaaacagac ccagaatgct gacggaatga ctctgtctgc tttctcaccc
     8521 ctatctgtca ccactgagca gcagggcccc tcctgaggcc tctgccacag ctctccccac
     8581 ccatcctccc agagggacca tgaagatttg gtctcctggg gctcctgtcc cagtttctgc
     8641 ttgtgcttcc cgtcaaggta ggactggaga gaggcaacta taatgaggtg aacgtggctg
     8701 gactcaccca gggctgggcg ggtggtgtag atgtaccctg ccctggactc ttctttccta
     8761 aactcattgg tcatctgccc taatgagttc actccttctg tatatcgtac ttgcatgccc
     8821 ccaaggcatg gaatttaaga ttagtaatct gagttcaagt tctagctctg acatttctga
     8881 tgtgaccctg ggcaggtcac tgaggaagct ctctgcaccc ccatctcatt ttctagaaaa
     8941 tcagaagaag aaccccatgg agatgaagtg acctaaggcc ataatgttac gaggtacctt
     9001 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9061 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9121 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9181 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9241 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9301 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9361 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9481 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9541 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9601 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9661 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9721 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9781 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9841 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9901 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9961 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    10021 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    10081 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    10141 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10201 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10261 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10321 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10381 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10441 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10501 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10561 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10621 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10681 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10741 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10801 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10861 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10921 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10981 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11041 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11101 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11161 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11221 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11281 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11341 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11401 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11461 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11521 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11581 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11641 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11701 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11761 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11821 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11881 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11941 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12001 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12061 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12121 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12181 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12241 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12301 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12361 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12421 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12481 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    12541 ctt
//
