LOCUS       sequence-366233-        9426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human MOSPD2 (mutant W201E, unable to bind lipid 
            droplets) fused to mScarlet in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Zouiouich M, Di Mattia T, Martinet A, Eichler J, Wendling C, 
            Tomishige N, Grandgirard E, Fuggetta N, Fromental-Ramain C, Mizzon 
            G, Dumesnil C, Carpentier M, Reina-San-Martin B, Mathelin C, Schwab 
            Y, Thiam AR, Kobayashi T, Drin G, Tomasetto C, Alpy F
  TITLE     MOSPD2 is an endoplasmic reticulum-lipid droplet tether functioning 
            in LD homeostasis.
  JOURNAL   J Cell Biol. 2022 Jun 6;221(6). pii: 213116. doi: 
            10.1083/jcb.202110044. Epub 2022 Apr 7.
  PUBMED    35389430
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-06-6"; volume: "221(6). pii: 213116. doi"; 
            pages: " 10.1083/jcb.202110044. Epub 2022 Apr 7"
COMMENT     Sequence Label: pQCXIP mScarlet-MOSPD2 W201E
FEATURES             Location/Qualifiers
     source          1..9426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             509..684
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    747..946
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1147..1563
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1493..1515
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1531..1547
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1600..1903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1904..2107
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2104..2128
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2227..2236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2233..2928
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2366..2384)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2634..2653
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(4549..5123,5124..5126,5127..5135)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4716..4733)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4943..4962
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5155..5754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5155..5174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5651..5671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6128..6553
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6656..6678)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6814..6834)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6819..7148
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6999..7134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7170..7189
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7270..7287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7441..8029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7521..7540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8200..8991,8992..9060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8823..8842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9061..9165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9233..9251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9351..9426,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctca ataaaagagc ccacaacccc tcactcggcg
      541 cgccagtctt ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc
      601 tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      661 tacccacgac gggggtcttt catttggggg ctcgtccggg atttggagac ccctgcccag
      721 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat
      781 tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt actagttagc taactagctc
      841 tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg
      901 agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg
      961 tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc
     1021 ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg
     1081 cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
     1141 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     1201 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     1261 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     1321 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     1381 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     1441 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     1501 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     1561 gccggaattg aagatctggg ggatcgatcc tctagagtcc gttacataac ttacggtaaa
     1621 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1681 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1741 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     1801 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     1861 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     1921 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     1981 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2041 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2101 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2161 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgga
     2221 cctgcagcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
     2281 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2341 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     2401 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt caccaagcac
     2461 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
     2521 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
     2581 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
     2641 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
     2701 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
     2761 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
     2821 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
     2881 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
     2941 tctcgagctc aagcttcgaa ttctgcagag aatcacgccc agaataaagc caagctcatc
     3001 tctgagaccc ggaggaggtt cgaagctgag tatgtgacag ataagtcaga taaatatgat
     3061 gcacgtgatg ttgaaaggct acaacaagat gataactggg ttgaaagtta cttatcttgg
     3121 agacataata ttgtagatga aacactgaag atgctcgatg agagttttca gtggaggaaa
     3181 gaaatttctg tcaatgacct taatgaatcc tccattccca gatggttatt ggaaattggt
     3241 gttatttatc tccatggtta tgacaaagaa ggtaacaaat tgttctggat cagggtgaag
     3301 tatcatgtaa aagaccagaa aaccatattg gacaaaaaga agctcatagc attctggttg
     3361 gaacgttatg ctaagaggga aaatgggaaa cctgtaacag tgatgtttga cctgtcagaa
     3421 actggaataa atagcattga catggacttt gtacgcttta tcatcaactg ctttaaggtt
     3481 tattacccta aatacctctc aaaaatagtg atctttgata tgccttggtt aatgaatgct
     3541 gctttcaaaa ttgtgaaaac cgaacttggt ccagaagcag tgagcttgtt gaagtttaca
     3601 agcaaaaatg aagtccagga ctatgtcagt gtagaatacc tgcctcccca catgggtgga
     3661 actgatcctt tcaagtatag ctatccacca ctagtagatg atgacttcca gaccccactg
     3721 tgtgagaatg ggcctattac cagtgaggat gaaacttcaa gtaaagaaga catagaaagt
     3781 gatggcaaag aaacattgga aacaatttct aatgaagaac aaacacctct tcttaaaaag
     3841 attaacccaa ccgaatctac ttccaaagca gaagaaaatg aaaaagttga ttcaaaagtg
     3901 aaagctttca agaaaccatt gagtgtattt aaaggcccct tactacacat cagcccagca
     3961 gaagaactgt actttggaag tacagaatcc ggagagaaga aaaccttaat agtgttgaca
     4021 aatgtaacta aaaatatagt ggcatttaag gtgagaacaa cagctccaga aaaatacaga
     4081 gtcaagccaa gcaatagcag ctgtgacccg ggtgcatcag tggatatagt tgtgtctccc
     4141 catgggggtt taacagtctc tgcccaagac cgttttctga taatggctgc agaaatggaa
     4201 cagtcatctg gcacaggccc agcagaatta actcagtttt ggaaagaagt tcccagaaac
     4261 aaagtgatgg aacataggtt aagatgccat actgttgaaa gcagtaaacc aaacactctt
     4321 acgttaaaag acaatgcttt caatatgtca gataaaacca gtgaagatat atgtctacaa
     4381 ctcagtcgtt tactagaaag caataggaag cttgaagacc aagttcagcg ttgtatctgg
     4441 ttccagcagc tgctgctttc cttaacaatg ctcttgcttg cttttgtcac ctctttcttc
     4501 tatttattgt acagttaagg atccggaatt ccgccccccc cccccccccc cctctccctc
     4561 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4621 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4681 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4741 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4801 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4861 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4921 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4981 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5041 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     5101 cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc
     5161 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     5221 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     5281 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5341 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5401 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5461 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5521 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5581 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5641 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5701 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     5761 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc cgaccgaagc
     5821 cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac tctagtcgag
     5881 ggctgcagcg ctgcagaggc cgagtgcaga actgctccaa agggacctca aggctttccg
     5941 agggacacta ggctgactcc atcgagccag tgtagagata agcttatcga ttagtccaat
     6001 ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
     6061 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
     6121 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     6181 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     6241 atggaacagg gtcgacccta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     6301 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     6361 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     6421 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     6481 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     6541 ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac
     6601 ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     6661 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6721 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     6781 cacgtagcga tagcggagtg tagatccggc tgtggaatgt gtgtcagtta gggtgtggaa
     6841 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6901 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6961 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7021 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7081 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7141 tttgcaaaaa gcttactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7201 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     7261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     8521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     8821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9181 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9241 tatcacgagg ccctttcgtc ttcaagaatt agcttggcca ttgcatacgt tgtatccata
     9301 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt
     9361 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9421 gttccg
//
