LOCUS       sequence-36624-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for NTAP (N-terminal affinity purification), 
            frame C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-NTAP-C (w324-1)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             140..217
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             236..349
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(419..435)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..436)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(446..545)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(657..676)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cttacnggnc caatatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg cttttttata atgccaactt tgtacaaaaa agcaggcttt aaaggaaccc
      121 ggtggcggcc gccaccatga agcgacgatg gaaaaagaat ttcatagccg tctcagcagc
      181 caaccgcttt aagaaaatct catcctccgg ggcacttgga agcggtagcg gtaccatgga
      241 cgagaagacc accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga
      301 gcagctgcgg gccaggctgg agcaccaccc tcagggccag cgggagccct ccggcggctg
      361 caagctgggc ttgcccgggc ggatcccccg ggctgcagga attcgatatc aagcttatcg
      421 ataccgtcga cctcgagata tctagaccca gctttcttgt acaaagttgg cattataaga
      481 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      541 atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
      601 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
      661 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccnacatgga
      721 tgctgantta tatgggtata aatgngctcg cgattaatgn cggcaatcan gtgcnacaat
      781 ctatcncttg tatgggnaa
//
