LOCUS       sequence-366260-        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the PKA FRET sensor AKAR3-EV in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Botman D, Kanagasabapathi S, Savakis P, Teusink B
  TITLE     Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in 
            budding yeast.
  JOURNAL   FEMS Yeast Res. 2023 Jan 4;23:foad029. doi: 10.1093/femsyr/foad029.
  PUBMED    37173282
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2023-01-4"; volume: "23:foad029. doi"; pages: " 
            10.1093/femsyr/foad029"
COMMENT     Sequence Label: pDRF1-GW AKAR3-EV
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(852..854,855..857,858..1565)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             2001..2348
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(2388..2390,2391..2393,2394..2906)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3117..3143
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      3303..3490
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3358..3382)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3502..3519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3502..3518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3511..3533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3954..5300
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5411..6214)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6215..6430)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6455..6473)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6541..6645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6646..6714,6715..7506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6864..6883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7677..8265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8166..8185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8419..8436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8553..8574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8589..8594,8595..8612,8613..8619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8627..8643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8632..8654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8651..8667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8651..8667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8697..9152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8834..8855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9046..9065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc acggagctca
      841 ctgaattcgg catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      901 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      961 atgccaccta cggcaagctg accctgaagc ttctatgcac caccggcaag ctgcccgtgc
     1021 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     1081 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1141 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1201 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1261 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1321 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1381 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1441 ccgacaacca ctacctgagc taccagtccg ccctgttcaa agaccccaac gagaagcgcg
     1501 atcacatggt cctgctggag ttcctgaccg ccgccgggat cactgagggc atgaacgagc
     1561 tgtacctcga gaagttttct caagaacaga tcggcgaaaa cattgtgtgc agggtcattt
     1621 gtaccacggg tcaaattccc atccgagatt tgtcagctga tatttcacaa gtgcttaagg
     1681 aaaaacgatc cataaagaaa gtttggacat ttggtagaaa cccagcctgt gactatcatt
     1741 taggaaacat ttcaagactg tcaaataagc atttccaaat actactagga gaagacggta
     1801 accttttatt gaatgacatt tccactaatg ggacctggtt aaatgggcaa aaagtcgaga
     1861 agaacagcaa tcagttactg tcccaaggtg atgaaataac cgttggtgta ggcgtggaat
     1921 cagatatttt atctctggtc attttcataa acgacaaatt taagcagtgc ctggagcaga
     1981 acaaagttga tcgcggtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     2041 ctggtggtag tgctggtggt tccggcagtg ctggtggtag tgctggtggt agtaccagtg
     2101 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg
     2161 gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg tggtagtgct ggtggtagtg
     2221 ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt agtgctggtg
     2281 gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     2341 ctggtggttc cggattgagg cgcgcgacgc tggttgacgg cggccgcatg gtgagcaagg
     2401 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2461 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2521 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2581 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2641 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2701 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2761 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2821 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     2881 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2941 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ttgagcaccc
     3001 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3061 tgaccgccgc cgggatcact ctcggcatgg acgagctggg ccgctctaga ctgcagctgc
     3121 ctcctctgga acgcctgact ctggattaaa gtgtcgaccg tggatcagct ttggacttct
     3181 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa
     3241 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat
     3301 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     3361 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     3421 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     3481 cctctaccgg catgccaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3541 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3601 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     3661 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taatcggatc
     3721 gtacttgtta cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag
     3781 atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat
     3841 gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat
     3901 ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag
     3961 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     4021 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     4081 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca
     4141 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     4201 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     4261 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     4321 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     4381 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     4441 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     4501 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     4561 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     4621 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     4681 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     4741 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     4801 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg
     4861 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag
     4921 ttcctatact ttctagctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     4981 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     5041 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     5101 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
     5161 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
     5221 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
     5281 atttcctttg atattggatc gatccgatga taagctgtca aacatgagaa ttgggtaata
     5341 actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata
     5401 atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc
     5461 tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa
     5521 taataatgtc agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg
     5581 cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat
     5641 ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg
     5701 caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt
     5761 ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac
     5821 cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta
     5881 ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc
     5941 tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc
     6001 cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac
     6061 ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca
     6121 agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag
     6181 tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcatgttt
     6241 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata
     6301 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga
     6361 ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg
     6421 atgaattgaa aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     6481 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8641 caatttcaca caggaaacag ctatgaccat gattacgcca agcttaccgc atcaggaaat
     8701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     8761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     9001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     9061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     9121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
//
