LOCUS       sequence-366262-       12579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generate lentivirus to introduce mClover tagged m6A binding null 
            mutant W377A/W428A YTHDC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 12579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pLenti-Ef1a-mClover-W377A/W428A
            YTHDC1-T2A-BSD...
FEATURES             Location/Qualifiers
     source          1..12579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3872..3881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3878..3880,3881..3883,3884..4594)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3923..3944)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4531..4552
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6794..6847
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6848..7246
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7271..7859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7324..7344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7742..7753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7862..7878)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7863..7879)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8384..8564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8590..8607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8596..8820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8866..9294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8953..8972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9163..9184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9303..9323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9308..9637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9488..9623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9550..9569
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9685..9732
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9751..10125
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10255..10376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10292..10311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10346..10365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10425..10441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10425..10441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10438..10460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10449..10465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10473..10479,10480..10497,10498..10503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10518..10539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10656..10673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10827..11415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10907..10926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11586..12377,12378..12446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12209..12228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12447..12551)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gtgagcaagg gcgaggagct
     3901 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3961 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     4021 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     4081 cgtggcctgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4141 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     4201 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4261 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4321 ccacaacgtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat
     4381 ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4441 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct
     4501 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4561 cgggattaca catggcatgg acgagctgta caaggcggct gacagtcggg aggagaaaga
     4621 tggagaactt aatgttctgg atgatatttt aactgaagta ccagaacaag atgatgaact
     4681 gtataatcca gagagtgaac aagataaaaa tgagaaaaag ggatcaaaaa gaaaaagtga
     4741 tcgaatggaa tctactgata ccaaacgaca aaagccttct gtccattcaa gacaactggt
     4801 ttctaagcca ctgagctcat ctgttagcaa taacaaaaga atagttagta caaaaggaaa
     4861 gtcagccaca gagtataaaa atgaggaata tcaaagatct gaaagaaaca agcgtctaga
     4921 tgctgatcgg aaaattcgtc tatcaagtag tgcctccaga gaaccttata agaatcaacc
     4981 tgaaaaaacc tgtgtccgga aaagggatcc tgaaaggagg gccaaatctc ctacgccaga
     5041 tggttctgag agaattgggc ttgaagtgga tagacgtgca agcagatcca gccagtcttc
     5101 taaggaagaa gtgaactctg aagaatatgg ctctgaccat gagactggca gcagtggttc
     5161 ttctgatgag caagggaaca acactgagaa tgaggaggaa ggagtggaag aagatgtgga
     5221 ggaagatgaa gaagtagaag aagatgcaga agaagatgaa gaggtggatg aagatggaga
     5281 ggaggaggag gaagaggagg aggaggaaga ggaggaggag gaggaggaag aagaagaata
     5341 tgaacaggat gagagagacc agaaagagga gggaaatgat tatgacactc gaagtgaggc
     5401 cagtgactct ggttctgaat ctgtttcctt cacagatggg tctgtcagat ctggttcagg
     5461 cacagatgga tcagatgaga aaaagaagga aaggaagaga gctagaggca tatctccaat
     5521 tgtttttgat agaagtggaa gctctgcatc agagtcatat gcaggttcag aaaagaagca
     5581 tgagaaatta tcatcttccg ttcgtgctgt ccgaaaagat caaaccagta aactcaaata
     5641 tgtgcttcaa gatgcaagat ttttcctcat aaagagtaac aaccatgaga atgtgtctct
     5701 tgccaaagcg aagggtgtag cgtccacgct ccctgtaaat gagaagaaat taaatcttgc
     5761 atttagatct gcaaggagtg ttatcttaat attttctgtc agagagagtg gaaaatttca
     5821 agggtttgca agactttctt cagaatcaca tcacggagga tctcctatac acgcggtgct
     5881 tccagcagga atgagtgcta aaatgctggg aggtgtcttt aaaattgact ggatttgcag
     5941 gcgtgaatta cccttcacta agtcggctca tctcaccaat ccttggaatg aacataaacc
     6001 agtaaagatc ggacgtgatg gacaggaaat tgaacttgaa tgtggaaccc agctttgtct
     6061 tctgtttccc cccgatgaaa gtattgactt gtatcaggtg atccacaaga tgagacacaa
     6121 gagaagaatg cattctcagc cccgatcacg aggacgtcca tcccgtcgag aaccagtccg
     6181 ggatgtggga aggcgtcgac cagaagatta tgatattcat aacagcagaa agaaaccaag
     6241 gattgactat ccccctgagt ttcaccagag accagggtat ttaaaggatc cacgatacca
     6301 ggaagtggac agacgatttt caggagttcg ccgagatgtg tttttaaatg ggtcctacaa
     6361 tgattatgtg agggaatttc ataacatggg accaccacca ccttggcaag gaatgccccc
     6421 ttacccagga atggaacaac ctccacacca tccttactat cagcaccatg ctccacctcc
     6481 tcaagctcat cccccttact caggacatca tccagtacca catgaagcaa gatacagaga
     6541 taaacgagta catgattatg atatgagggt ggatgatttc cttcgtcgca cacaagctgt
     6601 tgtcagtggc cggagaagta gaccccgtga aagagaccgg gaacgagagc gagaccgccc
     6661 tagagataac agacgagaca gagagcgaga tagaggacgt gatagagaaa gagaaagaga
     6721 gcgattatgt gatcgagaca gagaccgagg ggagagaggt cgatatagaa gaattaactg
     6781 tacaggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     6841 tggcccaatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     6901 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     6961 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     7021 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     7081 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     7141 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     7201 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagaat tcgatatcaa
     7261 gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7321 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7381 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7441 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7501 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7561 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7621 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7681 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7741 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7801 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7861 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7921 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7981 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8041 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     8101 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     8161 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8221 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     8281 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     8341 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     8401 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8461 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8521 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     8581 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     8641 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8701 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8761 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8821 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8881 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8941 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9001 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9061 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9121 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9181 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9241 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     9301 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9361 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9421 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9541 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     9601 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     9661 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     9721 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     9781 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     9841 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9901 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9961 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
    10021 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
    10081 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
    10141 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10201 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    10261 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10321 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10381 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    10441 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10501 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10561 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10621 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    11281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11521 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11581 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11641 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11701 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11761 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11821 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11881 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11941 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12001 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12061 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12121 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12181 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12241 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    12301 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    12361 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12421 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12481 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12541 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
