LOCUS       sequence-366264-       12070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express GFP tagged GluN2B from a 
            CMV promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..12070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2700..2702,2703..3413)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2700..2702,2703..3413)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2742..2763)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3003..3022)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3350..3371
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7781..7805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7858..7899
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7923..8511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7976..7996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8394..8405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8591..8824
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8896..9017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8933..8952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8987..9006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9057..9192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9212..9231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9213..9231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9241..9258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9241..9257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9250..9272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9399..9854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9486..9505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9696..9717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9880..9984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9985..10053,10054..10845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10203..10222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11016..11604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11505..11524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11758..11775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11892..11913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11928..11933,11934..11951,11952..11958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11966..11982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11971..11993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11990..12006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11990..12006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12025..12045
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12027..12045
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctacgccacc atgaagccca gcgcagagtg ctgttccccc aagttctggt
     2641 tggtgttggc cgtcttggcc gtatcaggca gcaaagctcg ttcccaaaag agcgctaggg
     2701 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2761 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2821 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2881 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2941 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3001 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3061 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3121 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3181 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3241 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3301 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3361 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggctagcc
     3421 cccccagcat cggcatcgct gtcatcctcg tgggcacttc agacgaagtg gccataaaag
     3481 acgcccacga gaaagatgac ttccatcatc tctcagtagt tccccgggtg gagctggtag
     3541 ccatgaacga aactgaccca aagagcatca tcacccgtat ctgcgatctt atgtctgacc
     3601 ggaagatcca gggggtggtg ttcgcggatg acaccgacca agaagccatc gctcagatcc
     3661 tcgacttcat ttctgctcag actctcaccc ccatcctggg catccatggg ggctcatcta
     3721 tgataatggc ggataaggat gagtcctcca tgttcttcca gtttggcccg tctatcgaac
     3781 agcaagcttc cgtcatgctc aacatcatgg aagaatatga ctggtacatc ttttccatcg
     3841 tcaccaccta cttccctggc taccaggact ttgtgaacaa gatccgcagt accatcgaga
     3901 acagcttcgt gggctgggag ctcgaggaag tcctcctgct agacatgtct ctggacgatg
     3961 gcgactctaa gattcagaat cagctgaaga agctccaaag ccccatcatt ctcctttatt
     4021 gcacgaagga ggaagccacc tacatttttg aagtagctaa ctcagttggg ctgactggct
     4081 acggctacac gtggattgtg ccgagtctgg tggccgggga tacggacacg gtgccttcag
     4141 agttccccac ggggcttatc tctgtgtctt atgatgaatg ggactatggc cttcctgcca
     4201 gagtgagaga tggaattgcc atcatcacca ctgctgcctc ggacatgctg tccgaacaca
     4261 gtttcatccc tgagcccaag agcagttgct acaacaccca cgagaagagg atctaccagt
     4321 ctaacatgtt gaataggtat ctgatcaatg tcacttttga agggagaaac ctgtccttca
     4381 gcgaagatgg ctaccagatg catccgaagc tggtgataat ccttctgaac aaggagagga
     4441 agtgggagag ggtggggaaa tggaaggaca agtccctgca gatgaagtat tatgtgtggc
     4501 ctcggatgtg tcctgagact gaggagcaag aggatgacca tctgagcatt gtcaccttgg
     4561 aggaggcgcc atttgtcatt gtggaaagcg tggaccctct cagtggaacc tgcatgagga
     4621 atacagtccc gtgccagaag cgcatcatct ctgagaataa aacagatgag gaaccaggct
     4681 acatcaaaaa atgctgcaag gggttctgta ttgacatcct taagaaaatt tctaagtctg
     4741 tgaagttcac ctatgacctt tacctggtga ccaatggcaa gcacgggaag aagattaatg
     4801 ggacctggaa tggcatgatc ggtgaggtgg tcatgaagag ggcctacatg gcagtgggat
     4861 cactaactat caatgaagaa cggtcagagg tggttgactt ctctgtaccc ttcatagaaa
     4921 ctggcatcag tgtcatggta tctcgcagca atgggactgt gtcaccttct gccttcttag
     4981 agccattcag cgctgacgtg tgggtgatga tgtttgtgat gctgctcatt gtttctgcgg
     5041 tggctgtctt tgtctttgaa tacttcagcc ctgtgggtta caacaggtgc ctagccgatg
     5101 gcagagagcc aggaggccca tctttcacca tcggcaaagc aatttggtta ctctggggtc
     5161 tggtgtttaa caactccgta cctgtgcaga acccaaaggg gaccacctcc aagatcatgg
     5221 tgtcagtgtg ggccttcttt gctgtcattt tcctggccag ctacactgcc aacttagcag
     5281 ccttcatgat ccaagaggag tatgtggacc aggtttctgg cctgagtgac aagaagttcc
     5341 agagacctaa tgacttctca ccccctttcc gctttgggac tgtgcccaat ggcagcacag
     5401 agaggaatat ccgtaataac tatgcagaaa tgcatgccta catgggaaag ttcaaccaaa
     5461 ggggtgtaga tgatgcattg ctctccctga aaacagggaa gcttgatgca ttcatctatg
     5521 atgcagctgt gctcaactac atggctggaa gggacgaagg ctgcaaactg gtgaccattg
     5581 gcagtggcaa ggtctttgct tctaccggct atggcattgc tatccaaaag gactccgggt
     5641 ggaagcgcca ggtggacctg gctatcctgc agctgtttgg agatggggag atggaagaac
     5701 tggaagctct ctggctcact ggcatttgcc acaatgagaa gaatgaggtg atgagcagcc
     5761 agctggacat cgacaatatg gcaggtgtct tctatatgtt gggggcagcc atggccctca
     5821 gcctcatcac cttcatctgt gagcatctgt tctattggca gttccggcat tgcttcatgg
     5881 gtgtctgttc tggcaagcct ggcatggtct tctccatcag cagaggtatc tacagctgta
     5941 tccatggggt agccatagag gagcgccaat ccgtgatgaa ctcccccact gccaccatga
     6001 acaacaccca ctccaacatc ctacgcttgc tccgcacggc caagaacatg gccaacctgt
     6061 ctggagtaaa cggctcccct cagagtgccc tggacttcat ccgccgagag tcctccgtct
     6121 acgacatctc tgagcatcgt cgcagcttca cgcattcaga ctgcaagtct tacaataacc
     6181 caccctgtga ggaaaacctg ttcagtgact acattagcga ggtagagaga acatttggta
     6241 acctgcagct gaaggacagc aatgtgtacc aagaccacta tcaccatcac caccggccac
     6301 acagcatcgg cagcaccagc tccattgatg ggctctatga ctgtgacaac ccacccttca
     6361 ccacccagcc caggtcaatc agcaagaaac ccctggacat cggcctgccc tcctccaaac
     6421 atagccagct cagcgacctg tatggcaagt tctctttcaa gagtgaccgc tacagtggcc
     6481 acgacgactt gattcgatcg gatgtctccg acatctccac gcacactgtc acctatggga
     6541 acatcgaggg caacgcagcc aagaggagga aacagcagta taaggacagt ctaaagaagc
     6601 ggccagcctc ggccaaatcg aggagggagt ttgatgaaat cgagctggcc taccgtcgcc
     6661 gaccaccccg ctccccggac cacaagcgct acttcaggga caaagaaggg ctccgagact
     6721 tctacctgga ccagttccga acaaaggaga actcgcctca ctgggagcac gtggacttga
     6781 ctgacattta caaagaacgc agtgacgact tcaagcgaga ttcggtcagt ggaggtgggc
     6841 cctgtaccaa caggtctcac ctcaaacacg gaacgggcga gaagcacgga gtggtaggcg
     6901 gggtgcctgc tccttgggag aagaacctga ccaatgtgga ttgggaggac cggtctgggg
     6961 gcaacttctg ccgcagctgt ccttccaagc tgcacaatta ctcctcgacg gtggcagggc
     7021 agaactcggg ccggcaggcc tgcatcagat gtgaggcctg taagaaggct ggtaacctgt
     7081 acgacatcag cgaggacaac tccctgcagg aactggacca gccggctgcc cccgtggctg
     7141 tgacatccaa cgcctccagc accaagtacc ctcaaagccc gactaattcc aaggctcaga
     7201 agaagaatcg gaacaaactg cgccggcagc attcctacga caccttcgtg gacctgcaga
     7261 aggaggaggc cgccttggcc ccacgcagcg tgagcctgaa agacaagggc cgattcatgg
     7321 atgggagccc ctacgcccat atgtttgaga tgccagctgg tgagagctcc tttgccaaca
     7381 agtcctcagt gcccactgcc ggacaccacc acaacaaccc cggcagcggc tacatgctca
     7441 gcaagtcgct ctaccctgac cgggtcacgc aaaacccttt catccccact tttggggatg
     7501 accagtgctt gcttcacggc agcaaatcct acttcttcag gcagcccacg gtggcagggg
     7561 cgtcaaaaac aaggccggac ttccgggccc ttgtcaccaa taagccagtg gtgtcagccc
     7621 ttcatggggc tgtgccaggt cgtttccaga aggacatttg tatagggaac cagtccaacc
     7681 cctgtgtgcc taacaacaaa aaccccaggg ctttcaatgg ctccagcaat ggacatgttt
     7741 atgagaaact ttctagtatt gagtctgatg tctgagtgag acccagcttt cttgtacaaa
     7801 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     7861 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgatcg
     7921 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7981 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     8041 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8101 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8161 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8221 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8281 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     8341 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8401 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8461 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggcgatg
     8521 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8581 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8641 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8821 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8881 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8941 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9001 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     9061 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9121 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9181 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     9241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     9301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     9361 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     9421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     9841 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9901 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9961 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    10021 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10081 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10141 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10201 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    10261 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10321 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10381 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10441 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10501 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10561 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10621 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10681 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10741 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10801 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10981 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    11041 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11101 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11161 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11221 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11281 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11341 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11401 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11461 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11521 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11581 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11641 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    11701 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    11761 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    11821 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    11881 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11941 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    12001 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    12061 ctgcaagctt
//
