LOCUS       sequence-366279-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A new version of er-(n2)oxStayGold(c4), which gives a higher 
            expression level. The StayGold gene has codon optimization for 
            mammalian expression. For brighter ER imaging if permitted..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, 
            Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, 
            Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A
  TITLE     A highly photostable and bright green fluorescent protein.
  JOURNAL   Nat Biotechnol. 2022 Apr 25. pii: 10.1038/s41587-022-01278-2. doi: 
            10.1038/s41587-022-01278-2.
  PUBMED    35468954
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Apr 25. pii: 10.1038/s41587-022-01278-2. doi: 
            10.1038/s41587-022-01278-2."
COMMENT     Sequence Label: pcDNA3/er-(n2)oxStayGold(c4)v2.0
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..732
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="MVSKGEELFTGVTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTL
                     LYPLLSDKSKCVEAYQNTIIKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHL"
ORIGIN
        1 atgctgctgc ccgtccccct gctgctgggc ctgctgggcg ccgccgcgga tccgatggtg
       61 agcaagggcg aggagctgtt tacaggcgtg acccccttca agttccagct gaagggcacc
      121 atcaacggca agagcttcac cgtggaaggc gagggcgagg gcaatagcca cgagggcagc
      181 cacaaaggca agtacgtgtg caccagcggc aaactgccaa tgtcttgggc cgccctggga
      241 actagcttcg gctatggcat gaagtactac accaagtacc ccagcggcct gaagaactgg
      301 ttccacgagg tgatgcccga gggcttcacc tacgacagac acatccagta caagggcgac
      361 ggcagcatcc acgccaagca ccagcacttc atgaagaacg gcacctacca caacatcgtg
      421 gagttcaccg gccaggactt caaggagaac agccccgtgc tgaccggcga catgaacgtg
      481 agcctgccca acgaggtgca gcacatcccc agagatgacg gcgtggagtg cccagtgacc
      541 ctgctgtacc ctctgctgag cgacaagagc aagtgcgtgg aggcctacca gaacaccatc
      601 atcaagcccc tgcacaatca gccagccccc gatgtgccat accactggat cagaaagcag
      661 tacacccaga gcaaggacga caccgaggag agagaccaca tcatccagag cgagaccctg
      721 gaggcccacc tgccatggca cgagccttct gcttctgccg tgaaggatga actgtaa
//
