LOCUS       sequence-366283-       11018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express Flag 
            Tagged-GluN2B(E1479Q) from a TRE3G promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 11018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..11018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1956..2334
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2509..2532
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6871..7459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6924..6944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7342..7353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7539..7772
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7844..7965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7881..7900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7935..7954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8005..8140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8160..8179)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8161..8179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8189..8206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8189..8205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8198..8220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8347..8802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8434..8453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8644..8665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8828..8932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8933..9001,9002..9793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9151..9170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9964..10552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10453..10472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10706..10723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10840..10861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10876..10881,10882..10899,10900..10906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10914..10930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10919..10941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10938..10954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10938..10954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10973..10993
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10975..10993
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtatcacga gactagcctc gagttggctt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaaggatcc
     2341 gccgccacca tgaagcccag cgcagagtgc tgttctccca agttctggtt ggtgttggcc
     2401 gtcttggccg tatcgggcag caaagctcgt tcccaaaaga gcgcccccag catcggcatc
     2461 gctgtcatcc tcgtgggcac ttccgacgaa gtggccataa aagatgccga ctacaaggac
     2521 gacgatgaca agcacgagaa agatgacttc catcatctct cagtagttcc ccgggtggag
     2581 ctggtagcca tgaacgagac tgacccaaag agcataatca cccgcatctg cgatcttatg
     2641 tctgaccgga agatccaggg ggtggtgttc gcggatgaca cggaccagga agccatcgcc
     2701 cagatcctcg atttcatttc tgctcagact ctcaccccca tcctgggcat ccatgggggc
     2761 tcatctatga taatggcaga taaggatgag tcctccatgt tcttccagtt tggcccatcc
     2821 attgaacagc aagcttctgt catgctcaac atcatggaag aatacgactg gtacatcttc
     2881 tccatcgtca ccacctactt ccccggctac caggacttcg tgaacaagat ccgcagcact
     2941 attgagaaca gctttgtagg ctgggagctc gaggaagtcc tcctgctaga catgtctcta
     3001 gacgatggcg actctaagat tcagaatcag ctgaagaagc tgcagagccc catcattctc
     3061 ctctactgca caaaggaaga agccacctac atcttcgaag tagctaactc agttgggctg
     3121 actggctacg gctacacatg gatcgtgccg agtctggtgg cgggggatac agacacggtg
     3181 ccttcagagt tccccacggg gctcatctct gtgtcatatg acgaatggga ctatggcctt
     3241 cctgccagag tgagagatgg gattgccatc atcaccactg ctgcctcgga catgctgtcc
     3301 gaacacagtt tcatccctga gcccaagagc agttgctaca acacccacga gaagaggatc
     3361 taccagtcta acatgctgaa taggtatctg atcaacgtca cttttgaagg gagaaacctg
     3421 tccttcagtg aagatggcta ccagatgcat ccgaagctgg tgataatcct tctgaacaag
     3481 gagaggaagt gggagagggt gggaaaatgg aaagacaagt ccctgcagat gaaatactac
     3541 gtgtggcctc gaatgtgtcc agagactgaa gaacaggaag atgaccatct gagcatcgtt
     3601 accttggagg aggcaccgtt tgtcattgtg gaaagtgtgg accctctcag tgggacctgc
     3661 atgcggaata cagtcccgtg ccagaagcgc atcatctctg agaataaaac agatgaggaa
     3721 ccaggctaca tcaaaaaatg ctgcaagggg ttttgtattg atatccttaa gaaaatttct
     3781 aagtctgtga agttcaccta tgacctttac ctggtgacca atggcaagca tggaaagaaa
     3841 atcaacggga cctggaacgg catgattggt gaggtggtca tgaagagggc ctacatggca
     3901 gtgggatcac taactatcaa tgaagaacgg tcagaggtgg ttgacttctc tgtgcccttc
     3961 atagagactg gcatcagtgt catggtatca cgcagcaatg ggactgtgtc accttctgcc
     4021 ttcttagagc cattcagtgc tgacgtgtgg gtgatgatgt ttgtgatgct gctcattgtc
     4081 tctgctgtag ctgtctttgt ctttgaatac ttcagccctg tgggttacaa ccggtgccta
     4141 gctgatggca gagagccagg cggcccatct ttcaccatcg gcaaagcgat ttggttactc
     4201 tggggtctgg tgtttaacaa ctccgtacct gtgcagaacc caaaggggac cacctccaag
     4261 atcatggtgt cagtgtgggc cttctttgct gtcattttcc tggccagcta cactgccaac
     4321 ttagccgcct tcatgatcca agaggagtat gtggaccagg tttccggcct gagtgacaag
     4381 aagttccaga gacctaatga cttctcaccc cctttccgct ttgggactgt gcccaatggc
     4441 agcacagaga ggaatatccg taataactat gcagaaatgc atgcctacat gggaaagttc
     4501 aaccaaaggg gtgtagatga tgccttgctc tccctgaaaa cagggaaact tgatgcattc
     4561 atctacgatg cagccgtgct caactacatg gctggaagag acgaaggctg caagctggtg
     4621 accattggca gtggcaaggt ctttgcttct acgggctatg gcattgctat ccaaaaagac
     4681 tctggttgga aacgccaggt ggaccttgct atcctgcagc tgtttggaga tggggagatg
     4741 gaagaactgg aagctctctg gctcactggc atttgccaca atgagaagaa tgaggttatg
     4801 agcagccagc tggacattga caacatggcg ggcgtcttct atatgttggg ggcagccatg
     4861 gctctcagcc tcatcacctt catctgtgaa catctcttct attggcagtt ccgacattgc
     4921 ttcatgggtg tctgttctgg caagcctggc atggtcttct ccatcagcag aggtatctac
     4981 agctgtatcc acggagtagc tatagaggag cgccaatccg tgatgaactc ccccactgcc
     5041 accatgaaca acacacactc caatatccta cgcttgctcc gaacggccaa aaacatggcc
     5101 aacctgtctg gagtcaacgg ctccccccag agtgccctgg acttcatccg ccgtgagtcc
     5161 tctgtctatg acatctctga gcatcgccgc agcttcacgc attcagactg caagtcgtac
     5221 aataacccac cctgtgagga aaacctgttc agtgactaca ttagtgaggt agagagaaca
     5281 tttggcaacc tgcagctgaa ggacagcaat gtgtaccaag accactatca ccatcaccac
     5341 cggccccaca gcatcggcag caccagctcc attgatgggc tctatgactg tgacaaccca
     5401 ccctttacca cccagcccag gtcaatcagc aagaaacccc tggacattgg cctgccctcc
     5461 tccaaacaca gccagctcag cgacctgtac ggcaagttct ctttcaagag tgaccgctac
     5521 agtggccatg atgacttgat tcgatcggat gtctcagaca tctccacgca tactgtcacc
     5581 tatggcaaca tcgagggcaa cgcagccaag aggaggaagc agcaatataa ggacagtcta
     5641 aagaagcggc cagcctcggc caaatctagg agggagtttg atgaaatcga gctggcctac
     5701 cgtcgccgac caccccgctc cccagaccac aagcgctact tcagggacaa agaagggctc
     5761 cgagacttct acctggacca gttccgaaca aaggagaact cgcctcactg ggagcacgtg
     5821 gacttaactg acatttacaa agaacgtagt gatgacttca agcgagattc ggtcagtgga
     5881 ggcgggccct gtaccaacag gtctcacctt aaacacggaa caggcgataa gcacggagtg
     5941 gtaggcgggg tgcctgctcc ttgggagaag aacctgacca atgtggattg ggaggatagg
     6001 tctgggggca acttctgccg cagctgtccc tccaagctgc acaattactc ctctacggtg
     6061 gcagggcaaa actcgggccg gcaggcctgc atcaggtgtg aggcctgcaa gaaggctggc
     6121 aacctgtatg acatcagcga ggacaactcc ctgcaggaac tggaccagcc tgctgcccct
     6181 gtggctgtgt catccaacgc ctccaccacc aagtatcctc aaagcccgac taattccaag
     6241 gcccagaaga agaatcggaa caaactgcgc cggcagcact cctacgacac cttcgtggac
     6301 ctgcagaagg aggaggccgc cttggcccca cgcagcgtga gcctgaaaga caagggccga
     6361 ttcatggatg ggagccccta cgcccatatg tttgagatgc cagctggtga gagctccttt
     6421 gccaacaagt cctcagtgac cactgccgga caccatcaca acaatcccgg cagcggctac
     6481 atgctcagca agtcgctcta ccctgaccgg gtcacgcaaa accctttcat ccccactttt
     6541 ggggatgatc agtgcttgct tcacggcagc aaatcctact tcttcaggca gcccacggtg
     6601 gcaggggcgt cgaaaacaag gccggacttc cgggcccttg tcaccaataa gccagtggtg
     6661 tcagcccttc atggggctgt gccaggtcgt ttccagaagg acatttgtat agggaaccag
     6721 tccaacccct gtgtgcctaa caacaaaaac cccagggctt tcaatggctc cagcaatgga
     6781 catgtttatg agaaactttc tagtattcag tctgatgtct gagtgagggg aattgatccg
     6841 gtacccgcgt accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa
     6901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7261 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7321 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7381 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7441 ctttgggccg cctccccgcc tggcgatggt acctttaaga ccaatgactt acaaggcagc
     7501 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7561 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7741 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7801 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7981 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8101 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     8161 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8221 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8281 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8341 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8401 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8461 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8521 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8581 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8641 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8701 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8761 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     8821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10621 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10681 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10741 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    10801 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10861 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10921 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    10981 ccctcactaa agggaacaaa agctggagct gcaagctt
//
