LOCUS       sequence-366315-       14986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown of murine KRAS gene by targeting the promoter region via 
            CRISPRi system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, 
            Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D, Hegde S, Gandhi 
            UH, Finch ER, Goodfield L, Quickel MD, Claxton DF, Paulson RF, 
            Prabhu KS
  TITLE     Activation of GPR44 decreases severity of myeloid leukemia via 
            specific targeting of leukemia initiating stem cells.
  JOURNAL   Cell Rep. 2023 Jul 25;42(7):112794. doi: 
            10.1016/j.celrep.2023.112794. Epub 2023 Jul 18.
  PUBMED    37459233
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-07-25"; volume: "42(7):112794. doi"; pages: " 
            10.1016/j.celrep.2023.112794. Epub 2023 Jul 18"
COMMENT     Sequence Label: pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2
FEATURES             Location/Qualifiers
     source          1..14986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..50
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             69..443
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    573..694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(610..629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     664..683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(743..759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(756..778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    767..783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(791..797,798..815,816..821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    836..857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(974..991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1145..1733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1225..1244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1904..2695,2696..2764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2527..2546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2765..2869)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2944..2963)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3135..3514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3516..3714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3669..3689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3732..3912
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3959..4084
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4577..4810
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4995..5039
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5337..5454
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(5498..5531)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(5542..5617)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5638..5886)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(5696..5715)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5866..5886)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        5919..7130
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6967..6986
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7159..7168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7168..7233
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7240..7260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7270..7296,7297..7299,7300..9783,9784..9789,9790..9855,
                     9856..9858,9859..11373)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(7270..7296,7297..7299,7300..9786,9787..9789,
                     9790..11373)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7270..11373
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11386..11406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11440..11634
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             11701..11721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11728..11781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(11788..11790,11791..12501)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(11788..11790,11791..12501)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(11830..11851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12091..12110)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12438..12459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(12517..12550)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    12575..13163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12628..12648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13046..13057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13166..13182)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13167..13183)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             13688..13868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13894..13911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13900..14124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14170..14598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14257..14276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14467..14488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14607..14627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14612..14941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14792..14927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14854..14873
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
       61 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
      121 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
      181 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
      241 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
      301 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
      361 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
      421 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
      481 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
      541 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
      601 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      661 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
      721 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      781 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      841 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      901 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      961 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1021 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1081 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1801 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2881 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     2941 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     3001 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     3061 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     3121 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     3181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     3241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3661 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     3721 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3901 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     3961 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     4021 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     4081 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     4141 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     4201 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     4261 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     4321 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     4381 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     4441 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     4501 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     4561 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     4621 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     4681 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     4741 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4801 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4861 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4921 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4981 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     5041 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     5101 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     5161 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     5221 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     5281 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     5341 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     5401 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     5461 tattacaggg acagcagaga tccagtttgg ttaattaata acttcgtata gcatacatta
     5521 tacgaagtta ttaaaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
     5581 tagccttatt ttaacttgct atttctagct ctaaaacccg ccacagcgct gccgcctggt
     5641 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
     5701 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
     5761 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattcc
     5821 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
     5881 gccctcttaa ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc gggttttggc
     5941 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     6001 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     6061 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     6121 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     6181 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     6241 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     6301 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     6361 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     6421 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     6481 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     6541 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     6601 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     6661 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     6721 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     6781 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     6841 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     6901 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     6961 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     7021 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     7081 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg
     7141 ctgcaggtcg actctagagc caccatggac tacaaagacc atgacggtga ttataaagat
     7201 catgacatcg attacaagga tgacgatgac aagatggccc ccaagaagaa gaggaaggtg
     7261 ggccgcggaa tggacaagaa gtactccatt gggctcgcca tcggcacaaa cagcgtcggc
     7321 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat
     7381 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggaa
     7441 accgccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat
     7501 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc
     7561 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca
     7621 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat
     7681 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg
     7741 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac
     7801 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa
     7861 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc
     7921 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg
     7981 tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac
     8041 ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat
     8101 ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca
     8161 gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg
     8221 agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt gctgaaggcc
     8281 cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat
     8341 ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa atttattaag
     8401 cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat
     8461 ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat tcacctgggc
     8521 gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg
     8581 gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg
     8641 ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac
     8701 ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac
     8761 tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac
     8821 ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca
     8881 gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg
     8941 aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct
     9001 gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc
     9061 ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag
     9121 gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa
     9181 acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg ccgatataca
     9241 ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca gagtggaaag
     9301 acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat gcagttgatc
     9361 catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg
     9421 gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata
     9481 ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag
     9541 aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca gaagaacagt
     9601 agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag
     9661 gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag
     9721 aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac
     9781 gtggatgcca tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg
     9841 acaagatccg ataaaaatag agggaagagt gataacgtcc cctcagaaga agttgtcaag
     9901 aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc
     9961 gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc cggcttcatc
    10021 aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca
    10081 cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact
    10141 ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag
    10201 atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt
    10261 atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat
    10321 gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc
    10381 ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt
    10441 cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt
    10501 agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag
    10561 accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag gaacagcgac
    10621 aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct
    10681 acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc
    10741 aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac
    10801 cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag
    10861 cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg
    10921 ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat
    10981 ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg
    11041 ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc
    11101 aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac
    11161 agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac
    11221 ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa gcggtacacc
    11281 tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa
    11341 acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa gaagaagagg
    11401 aaggtggcta gcgatgctaa gtcactgact gcctggtccc ggacactggt gaccttcaag
    11461 gatgtgtttg tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc
    11521 ctgtacagaa atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt
    11581 actaagccag atgtgatcct ccggttggag aagggagaag agccctggct ggtggagaga
    11641 gaaattcacc aagagaccca tcctgattca gagactgcat ttgaaatcaa atcatcagtt
    11701 ccgaaaaaga aacgcaaagt tgctagcgag ggcagaggaa gtcttctaac atgcggtgac
    11761 gtggaggaga atcccggccc tggtaccgtg agcaagggcg aggagctgtt caccggggtg
    11821 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
    11881 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
    11941 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
    12001 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
    12061 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
    12121 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
    12181 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
    12241 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
    12301 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
    12361 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
    12421 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
    12481 ggcatggacg agctgtacaa gaccggttga taatagataa cttcgtatag catacattat
    12541 acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
    12601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    12661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    12721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    12781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    12841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    12901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    12961 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
    13021 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    13081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    13141 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
    13201 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
    13261 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
    13321 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    13381 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    13441 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
    13501 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
    13561 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
    13621 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
    13681 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    13741 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    13801 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    13861 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
    13921 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
    13981 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
    14041 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
    14101 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
    14161 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    14221 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    14281 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    14341 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    14401 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    14461 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    14521 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    14581 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    14641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    14701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    14761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    14821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    14881 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    14941 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcac
//
