LOCUS       sequence-366316-       10510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test macrophage engulfment initiation. Lacks the FcyR chain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK72
FEATURES             Location/Qualifiers
     source          1..10510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(109..125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..126)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             216..449
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(474..492)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(475..492)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(782..801)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1015..1034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1134..1156
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1194..1212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1280..1384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1385..1453,1454..2245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1603..1622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2416..3004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2905..2924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3158..3175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3245..3265)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3250..3579
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3430..3565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4713..4778
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4908..4928
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5353..5487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5390..5409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5444..5463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5655..6288
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6335..6460
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6957..7190
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7375..7419
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7704..7821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7974..8381
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8493..8519
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8550..9092
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             join(9249..9251,9252..9254,9255..9968)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9294..9315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9555..9574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9902..9923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10028..10510,1..106)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10081..10101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10499..10510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
       61 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
      121 cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
      181 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      241 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
      301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      421 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
      481 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
      541 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
      601 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
      661 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
      721 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      781 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      841 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      901 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      961 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     1021 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     1081 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1141 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     1201 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     1261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1621 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1921 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2101 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2521 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2641 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2761 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2821 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2881 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2941 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3001 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3061 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3121 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3181 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3241 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3301 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3361 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3421 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3481 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3541 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     3601 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     3661 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     3721 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3781 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3841 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     3901 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     3961 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     4021 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     4081 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     4141 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     4201 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     4261 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     4321 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     4381 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     4441 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     4501 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     4561 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     4621 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     4681 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     4741 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     4801 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     4861 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     4921 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     4981 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     5041 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     5101 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     5161 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     5221 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     5281 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5341 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5461 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     5521 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     5581 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     5641 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     5701 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     5761 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     5821 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     5881 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     5941 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     6001 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     6061 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     6121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6181 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6301 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6361 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6421 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6481 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6541 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6601 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6661 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6721 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6781 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     6841 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6901 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     6961 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     7021 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     7081 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     7141 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     7201 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     7261 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     7321 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     7381 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     7441 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7501 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7561 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     7621 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     7681 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     7741 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7801 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
     7861 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc
     7921 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc
     7981 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
     8041 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
     8101 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     8161 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     8221 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     8281 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     8341 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga
     8401 gaattctcac gcgtgccgcc accatgcagt cgggcactca ctggagagtt ctgggcctct
     8461 gcctcttatc agttggcgtt tgggggcaag attacccata cgacgtccca gactacgctc
     8521 tgcccgagac cggcggcggc ggcggcggcg acaaagactg cgaaatgaag cgcaccaccc
     8581 tggatagccc tctgggcaag ctggaactgt ctgggtgcga acagggcctg caccgtatca
     8641 tcttcctggg caaaggaaca tctgccgccg acgccgtgga agtgcctgcc ccagccgccg
     8701 tgctgggcgg accagagcca ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc
     8761 agcctgaggc catcgaggag ttccctgtgc cagccctgca ccacccagtg ttccagcagg
     8821 agagctttac ccgccaggtg ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca
     8881 tcagctacag ccacctggcc gccctggccg gcaatcccgc cgccaccgcc gccgtgaaaa
     8941 ccgccctgag cggaaatccc gtgcccattc tgatcccctg ccaccgggtg gtgcagggcg
     9001 acctggacgt ggggggctac gagggcgggc tcgccgtgaa agagtggctg ctggcccacg
     9061 agggccacag actgggcaag cctgggctgg gtggcggaag cggaggtagt ggtggaagcc
     9121 ccccagacca cattccttgg attacagctg tacttccaac agttattata tgtgtgatgg
     9181 ttttctgtct aattctatgg aaatggaaga agaagaagcg gcctcggagc gcagacgcca
     9241 gcggcggcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     9301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     9361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     9421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     9481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     9541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     9721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     9781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     9841 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     9901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9961 acaagtaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
    10021 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    10081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    10141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    10201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    10261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    10321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    10381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    10441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    10501 ggccctcaat
//
