LOCUS       sequence-366320-       18529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but 
            with Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 18529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelCMENS1-Cre
FEATURES             Location/Qualifiers
     source          1..18529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..43
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    61..459
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    719..1192
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1265..2236)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2236..3411)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3737..3987
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3990..4745)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4836..5055
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5123..5654
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5131..5745
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6603..6705
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6706..7365
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6772..6791)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    7585..7606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7621..7626,7627..7644,7645..7651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7664..7686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7683..7699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7683..7699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8136..8154
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8136..8153
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             8353..9384
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSG
                     LPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLA
                     FLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLV
                     ERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSG
                     QRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLL
                     EDGD"
     primer_bind     17819..17838
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(18202..18221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18203..18221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18228..18245)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18228..18244)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18237..18259)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(18452..18471)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatcgaata taacttcgta taatgtatgc tatacgaagt tattagcgat gagctcggac
       61 ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagctc
      121 gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
      181 tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
      241 gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
      301 ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
      361 tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaagcaa cttcagacaa
      421 tacaaatcag cgacactgaa tacggggcaa cctcatgtcc gagctcgcga gctcgtcgac
      481 agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag
      541 gtattttgtc cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc
      601 cacagcaggc atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt
      661 caatacttgc ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga
      721 caatacaagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg
      781 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
      841 tcagaccgac gatacgagtg ggaccgtggt tccagactaa taatcagacc gacgatacga
      901 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac catggtccca
      961 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     1021 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     1081 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagtctgatt
     1141 atcagaccga cgatacaagt ggaacagtgg gcccagagag aatattcagg ccagttatgc
     1201 tttctggcct gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt
     1261 cgcatcaggg tgctggcttt tcaagttcct taagaatggc ctcaattttc tctatacact
     1321 cagttggaac acgagacctg tccaggttaa gcaccatttt atcgccctta tacaatactg
     1381 tcgctccagg agcaaactga tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt
     1441 taagcacaga agttaaaaga gtgataactt cttcagcttc aaatatcacc ccagcttttt
     1501 tctgctcatg aaggttagat gcctgctgct taagtaattc ctctttatct gtaaaggctt
     1561 tttgaagtgc atcacctgac cgggcagata gttcaccggg gtgagaaaaa agagcaacaa
     1621 ctgatttagg caatttggcg gtgttgatac agcgggtaat aatcttacgt gaaatatttt
     1681 ccgcatcagc cagcgcagaa atatttccag caaattcatt ctgcaatcgg cttgcataac
     1741 gctgaccacg ttcataagca cttgttgggc gataatcgtt acccaatctg gataatgcag
     1801 ccatctgctc atcatccagc tcgccaacca gaacacgata atcactttcg gtaagtgcag
     1861 cagctttacg acggcgactc ccatcggcaa tttctatgac accagatact cttcgaccga
     1921 acgccggtgt ctgttgacca gtcagtagaa aagaagggat gagatcatcc agtgcgtcct
     1981 cagtaagcag ctcctggtca cgttcattac ctgaccatac ccgagaggtc ttctcaacac
     2041 tatcaccccg gagcacttca agagtaaact tcacatcccg accacataca ggcaaagtaa
     2101 tggcattacc gcgagccatt actcctacgc gcgcaattaa cgaatccacc atcggggcag
     2161 ctggtgtcga taacgaagta tcttcaaccg gttgagtatt gagcgtatgt tttggaataa
     2221 caggcgcacg cttcattatc taatctccca gcgtggttta atcagacgat cgaaaatttc
     2281 attgcagaca ggttcccaaa tagaaagagc atttctccag gcaccagttg aagagcgttg
     2341 atcaatggcc tgttcaaaaa cagttctcat ccggatctga cctttaccaa cttcatccgt
     2401 ttcacgtaca acatttttta gaaccatgct tccccaggca tcccgaattt gctcctccat
     2461 ccacggggac tgagagccat tactattgct gtatttggta agcaaaatac gtacatcagg
     2521 ctcgaaccct ttaagatcaa cgttcttgag cagatcacga agcatatcga aaaactgcag
     2581 tgcggaggtg tagtcaaaca actcagcagg cgtgggaaca atcagcacat cagcagcaca
     2641 tacgacatta atcgtgccga tacccaggtt aggcgcgctg tcaataacta tgacatcata
     2701 gtcatgagca acagtttcaa tggccagtcg gagcatcagg tgtggatcgg tgggcagttt
     2761 accttcatca aatttgccca ttaactcagt ttcaatacgg tgcagagcca gacaggaagg
     2821 aataatgtca agccccggcc agcaagtggg ctttattgca taagtgacat cgtccttttc
     2881 cccaagatag aaaggcagga gagtgtcttc tgcatgaata tgaagatctg gtacccatcc
     2941 gtgatacatt gaggctgttc cctgggggtc gttaccttcc acgagcaaaa cacgtagccc
     3001 cttcagagcc agatcctgag caagatgaac agaaactgag gttttgtaaa cgccaccttt
     3061 atgggcagca accccgatca ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc
     3121 aaacacatca cgcatatgat taatttgttc aattgtataa ccaacacgtt gctcaacccg
     3181 tcctcgaatt tccatatccg ggtgcggtag tcgccctgct ttctcggcat ctctgatagc
     3241 ctgagaagaa accccaacta aatccgctgc ttcacctatt ctccagcgcc gggttatttt
     3301 cctcgcttcc gggctgtcat cattaaactg tgcaatggcg atagccttcg tcatttcatg
     3361 accagcgttt atgcactggt taagtgtttc catgagtttc attctgaaca tcctttaatc
     3421 attgctttgc gtttttttat taaatcttgc aatttactgc aaagcaacaa caaaatcgca
     3481 aagtcatcaa aaaaccgcaa agttgtttaa aataagagca acactacaaa aggagataag
     3541 aagagcacat acctcagtca cttattatca ctagcgctcg ccgcagccgt gtaaccgagc
     3601 atagcgagcg aactggcgag gaagcaaaga agaactgttc tgtcagatag ctcttacgct
     3661 cagcgcaaga agaaatatcc accgtgggaa aaactccagg tagaggtaca cacgcggata
     3721 gccaattcag agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc
     3781 cgatatcaag tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc
     3841 aaatttggct tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa
     3901 ggaaacagca aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac
     3961 cctcaaatct gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg
     4021 aaggaaaata cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc
     4081 attggagttc tgctgttgat ctcattaaca cagacctgca ggaagcggcg gcggaagtca
     4141 ggcatacgct ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga
     4201 gagacgatgc ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg
     4261 gcatacggat tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt
     4321 aacccgataa agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat
     4381 ggactgtgcg cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca
     4441 tcctcttcag ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt
     4501 atatccttac tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg
     4561 atctcgcaga taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg
     4621 aacagataca gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg
     4681 tttgactgga cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc
     4741 gccatgacag atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag
     4801 agtcaataag cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca
     4861 gggttaaggg caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa
     4921 agggcaattt gtcacaacac cttctctaga accagcatgg ataaaggcct acaaggcgct
     4981 ctaaaaaaga agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggataa
     5041 gtggataacc ccaagggaag ttttttcagg catcgtgtgt aagcagaata tataagtgct
     5101 gttccctggt gcttcctcgc tcactcgacc gggagggttc gagaaggggg ggcacccccc
     5161 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     5221 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     5281 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     5341 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     5401 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     5461 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     5521 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     5581 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     5641 caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     5701 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagccgag ggcttcgccc
     5761 tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat catggttctg
     5821 tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa cgtaacaccg
     5881 cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg
     5941 catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat taataatgcg
     6001 gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag tggataacag
     6061 ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca tttccatata
     6121 aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca tcagactccg
     6181 gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga
     6241 cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc agcggtatac
     6301 cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg accggaacag
     6361 agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag gtcctgaccg
     6421 ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga cggttacgcc
     6481 gctccatgag cttatcgcga ataaatacct gtgacggaag atcacttcgc agaataaata
     6541 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     6601 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     6661 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     6721 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     6781 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     6841 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     6901 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     6961 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     7021 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     7081 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     7141 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     7201 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     7261 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     7321 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     7381 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     7441 ggcagaaatt cgatgataag ctgtcaaaca tgagaattgg tcgacggccc gggcggccgc
     7501 aaggagttcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7561 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7621 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7681 cacaggaaac agctatgacc atgattacgc caagctattt aggtgagact atagaatact
     7741 caagcttgca tgcctgcagg tcgactctag aggatcccac attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtgt cgacgcggcc gctagcgatg
     7921 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
     7981 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
     8041 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
     8101 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagagtaaat
     8161 cctgtgtgct aattgaggtg cattggtctg caaatcgagt tgctaggcaa taaacacatt
     8221 tggattaatt ttaatcgttc gttgagcgat tagcagagaa ctgaccagaa catgtctggt
     8281 cgtaaagctc agggaaaaac cctgggcgtc aatatggtac gacgaggagt tcgctccttg
     8341 tcaaacgagc tcatggtgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
     8401 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
     8461 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
     8521 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     8581 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     8641 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     8701 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     8761 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     8821 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     8881 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     8941 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     9001 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     9061 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     9121 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     9181 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     9241 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     9301 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     9361 gtgcgcctgc tggaagatgg cgataccggt aactttgacc ttctcaagtt ggccggcgac
     9421 gtcgagtcca acccagggcc cctgcagcaa attttcgtga agaccctgac gggcaagacc
     9481 atcactcttg aggtcgagcc cagtgacacc atcgagaatg tcaaggccaa gatccaagac
     9541 aaggaaggca tcccacctga ccagcagagg ctgatattcg cgggcaaaca gctggaggat
     9601 ggccgcaccc tgtccgacta caacatccag aaagagtcca ccttgcacct ggtgctgtgt
     9661 ctccgcggtg gaatgtccgg tcgtaaagcc cagggcaaaa ccctaggcgt aaatatggtc
     9721 cgacgcggag tccgcaacat gacaatgtcc atgagcatga tcttggtagg agtgatcatg
     9781 atgtttttgt ctctaggagt tggggcggat caaggatgcg ccatcaactt tggcaagaga
     9841 gagctcggat cacggtccgg gacacgcgtg tacatggacg cagtctttga atacaccata
     9901 gactgcgatg gatctatctt gggtgcagcg gtgaacggaa aaaagagtgc ccatggctct
     9961 ccaacatttt ggatgggaag tcatgaagta aatgggacat ggatgatcca caccttggag
    10021 gcattagatt acaaggagtg tgagtggcca ctgacacata cgattggaac atcagttgaa
    10081 gagagtgaaa tgttcatgcc gagatcaatc ggaggcccag ttagctctca caatcatatc
    10141 cctggataca aggttcagac gaacggacct tggatgcagg taccactaga agtgaagaga
    10201 gaagcttgcc cagggactag cgtgatcatt gatggcaact gtgatggacg gggaaaatca
    10261 accagatcca ccacggatag cgggaaagtt attcctgaat ggtgttgccg ctcctgcaca
    10321 atgccgcctg tgagcttcca tggtagtgat gggtgttggt atcccatgga aattaggcca
    10381 aggaaaacgc atgaaagcca tctggtgcgc tcctgggtta cagctggaga aatacatgct
    10441 gtcccttttg gtttggtgag catgatgata gcaatggaag tggtcctaag gaaaagacag
    10501 ggaccaaagc aaatgttggt tggaggagta gtgctcttgg gagcaatgct ggtcgggcaa
    10561 gtaactctcc ttgatttgct gaaactcaca gtggctgtgg gattgcattt ccatgagatg
    10621 aacaatggag gagacgccat gtatatggcg ttgattgctg ccttttcaat cagaccaggg
    10681 ctgctcatcg gctttgggct caggacccta tggagccctc gggaacgcct tgtgctgacc
    10741 ctaggagcag ccatggtgga gattgccttg ggtggcgtga tgggcggcct gtggaagtat
    10801 ctaaatgcag tttctctctg catcctgaca ataaatgctg ttgcttctag gaaagcatca
    10861 aataccatct tgcccctcat ggctctgttg acacctgtca ctatggctga ggtgagactt
    10921 gccgcaatgt tcttttgtgc catggttatc ataggggtcc ttcaccagaa tttcaaggac
    10981 acctccatgc agaagactat acctctggtg gccctcacac tcacatctta cctgggcttg
    11041 acacaacctt ttttgggcct gtgtgcattt ctggcaaccc gcatatttgg gcgaaggagt
    11101 atcccagtga atgaggcact cgcagcagct ggtctagtgg gagtgctggc aggactggct
    11161 tttcaggaga tggagaactt ccttggtccg attgcagttg gaggactcct gatgatgctg
    11221 gttagcgtgg ctgggagggt ggatgggcta gagctcaaga agcttggtga agtttcatgg
    11281 gaagaggagg cggagatcag cgggagttcc gcccgctatg atgtggcact cagtgaacaa
    11341 ggggagttca agctgctttc tgaagagaaa gtgccatggg accaggttgt gatgacctcg
    11401 ctggccttgg ttggggctgc cctccatcca tttgctcttc tgctggtcct tgctgggtgg
    11461 ctgtttcatg tcaggggagc gcgcagaagt ggtcaccgga gaagtgggga tgtcttgtgg
    11521 gatattccca ctcctaagat catcgaggaa tgtgaacatc tggaggatgg gatttatggc
    11581 atattccagt caaccttctt gggggcctcc cagcgaggag tgggagtggc acagggaggg
    11641 gtgttccaca caatgtggca tgtcacaaga ggagctttcc ttgtcaggaa tggcaagaag
    11701 ttgattccat cttgggcttc agtaaaggaa gaccttgtcg cctatggtgg ctcatggaag
    11761 ttggaaggca gatgggatgg agaggaagag gtccagttga tcgcggctgt tccaggaaag
    11821 aacgtggtca acgtccagac aaaaccgagc ttgttcaaag tgaggaatgg gggagaaatc
    11881 ggggctgtcg ctcttgacta tccgagtggc acttcaggat ctcctattgt taacaggaac
    11941 ggagaggtga ttgggctgta cggcaatggc atccttgtcg gtgacaactc cttcgtgtcc
    12001 gccatatccc agactgaggt gaaggaagaa ggaaaggagg agctccaaga gatcccgaca
    12061 atgctaaaga aaggaatgac aactgtcctt gattttcatc ctggagctgg gaagacaaga
    12121 cgtttcctcc cacagatctt ggccgagtgc gcacggagac gcttgcgcac tcttgtgttg
    12181 gcccccacca gggttgttct ttctgaaatg aaggaggctt ttcacggcct ggacgtgaaa
    12241 ttccacacac aggctttttc cgctcacggc agcgggagag aagtcattga tgccatgtgc
    12301 catgccaccc taacttacag gatgttggaa ccaactaggg ttgttaactg ggaagtgatc
    12361 attatggatg aagcccattt tttggatcca gctagcatag ccgctagagg ttgggcagcg
    12421 cacagagcta gggcaaatga aagtgcaaca atcttgatga cagccacacc gcctgggact
    12481 agtgatgaat ttccacattc aaatggtgaa atagaagatg ttcaaacgga catacccagt
    12541 gagccctgga acacagggca tgactggatc ctggctgaca aaaggcccac ggcatggttc
    12601 cttccatcca tcagagctgc aaatgtcatg gctgcctctt tgcgtaaggc tggaaagagt
    12661 gtggtggtcc tgaacaggaa aacctttgag agagaatacc ccacgataaa gcagaagaaa
    12721 cctgacttta tattggccac tgacatagct gaaatgggag ccaacctttg cgtggagcga
    12781 gtgctggatt gcaggacggc ttttaagcct gtgcttgtgg atgaagggag gaaggtggca
    12841 ataaaagggc cacttcgtat ctccgcatcc tctgctgctc aaaggagggg gcgcattggg
    12901 agaaatccca acagagatgg agactcatac tactattctg agcctacaag tgaaaataat
    12961 gcccaccacg tctgctggtt ggaggcctca atgctcttgg acaacatgga ggtgaggggt
    13021 ggaatggtcg ccccactcta tggcgttgaa ggaactaaaa caccagtttc ccctggtgaa
    13081 atgagactga gggatgacca gaggaaagtc ttcagagaac tagtgaggaa ttgtgacctg
    13141 cccgtttggc tttcgtggca agtggccaag gctggtttga agacgaatga tcgtaagtgg
    13201 tgttttgaag gccctgagga acatgagatc ttgaatgaca gcggtgaaac agtgaagtgc
    13261 agggctcctg gaggagcaaa gaagcctctg cgcccaaggt ggtgtgatga aagggtgtca
    13321 tctgaccaga gtgcgctgtc tgaatttatt aagtttgctg aaggtaggag gggagctgct
    13381 gaagtgctag ttgtgctgag tgaactccct gatttcctgg ctaaaaaagg tggagaggca
    13441 atggatacca tcagtgtgtt cctccactct gaggaaggct ctagggctta ccgcaatgca
    13501 ctatcaatga tgcctgaggc aatgacaata gtcatgctgt ttatactggc tggactactg
    13561 acatcgggaa tggtcatctt tttcatgtct cccaaaggca tcagtagaat gtctatggcg
    13621 atgggcacaa tggccggctg tggatatctc atgttccttg gaggcgtcaa acccactcac
    13681 atctcctatg tcatgctcat attctttgtc ctgatggtgg ttgtgatccc cgagccaggg
    13741 caacaaaggt ccatccaaga caaccaagtg gcatacctca ttattggcat cctgacgctg
    13801 gtttcagcgg tggcagccaa cgagctaggc atgctggaga aaaccaaaga ggacctcttt
    13861 gggaagaaga acttaattcc atctagtgct tcaccctgga gttggccgga tcttgacctg
    13921 aagccaggag ctgcctggac agtgtacgtt ggcattgtta caatgctctc tccaatgttg
    13981 caccactgga tcaaagtcga atatggcaac ctgtctctgt ctggaatagc ccagtcagcc
    14041 tcagtccttt ctttcatgga caaggggata ccattcatga agatgaatat ctcggtcata
    14101 atgctgctgg tcagtggctg gaattcaata acagtgatgc ctctgctctg tggcataggg
    14161 tgcgccatgc tccactggtc tctcatttta cctggaatca aagcgcagca gtcaaagctt
    14221 gcacagagaa gggtgttcca tggcgttgcc aagaaccctg tggttgatgg gaatccaaca
    14281 gttgacattg aggaagctcc tgaaatgcct gccctttatg agaagaaact ggctctatat
    14341 ctccttcttg ctctcagcct agcttctgtt gccatgtgca gaacgccctt ttcattggct
    14401 gaaggcattg tcctagcatc agctgcctta gggccgctca tagagggaaa caccagcctt
    14461 ctttggaatg gacccatggc tgtctccatg acaggagtca tgagggggaa tcactatgct
    14521 tttgtgggag tcatgtacaa tctatggaag atgaaaactg gacgccgggg gagcgcgaat
    14581 ggaaaaactt tgggtgaagt ctggaagagg gaactgaatc tgttggacaa gcgacagttt
    14641 gagttgtata aaaggaccga cattgtggag gtggatcgtg atacggcacg caggcatttg
    14701 gccgaaggga aggtggacac cggggtggcg gtctccaggg ggaccgcaaa gttaaggtgg
    14761 ttccatgagc gtggctatgt caagctggaa ggtagggtga ttgacctggg gtgtggccgc
    14821 ggaggctggt gttactacgc tgctgcgcaa aaggaagtga gtggggtcaa aggatttact
    14881 cttggaagag acggccatga gaaacccatg aatgtgcaaa gtctgggatg gaacatcatc
    14941 accttcaagg acaaaactga tatccaccgc ctagaaccag tgaaatgtga cacccttttg
    15001 tgtgacattg gagagtcatc atcgtcatcg gtcacagagg gggaaaggac cgtgagagtt
    15061 cttgatactg tagaaaaatg gctggcttgt ggggttgaca acttctgtgt gaaggtgtta
    15121 gctccataca tgccagatgt tcttgagaaa ctggaattgc tccaaaggag gtttggcgga
    15181 acagtgatca ggaaccctct ctccaggaat tccactcatg aaatgtacta cgtgtctgga
    15241 gcccgcagca atgtcacatt tactgtgaac caaacatccc gcctcctgat gaggagaatg
    15301 aggcgtccaa ctggaaaagt gaccctggag gctgacgtca tcctcccaat tgggacacgc
    15361 agtgttgaga cagacaaggg acccctggac aaagaggcca tagaagaaag ggttgagagg
    15421 ataaaatctg agtacatgac ctcttggttt tatgacaatg acaaccccta caggacctgg
    15481 cactactgtg gctcctatgt cacaaaaacc tcaggaagtg cggcgagcat ggtaaatggt
    15541 gttattaaaa ttctgacata tccatgggac aggatagagg aggtcacaag aatggcaatg
    15601 actgacacaa ccccttttgg acagcaaaga gtgtttaaag aaaaagttga caccagagca
    15661 aaggatccac cagcgggaac taggaagatc atgaaagttg tcaacaggtg gctgttccgc
    15721 cacctggcca gagaaaagaa ccccagactg tgcacaaagg aagaatttat tgcaaaagtc
    15781 cgaagtcatg cagccattgg agcttacctg gaagaacaag aacagtggaa gactgccaat
    15841 gaggctgtcc aagacccaaa gttctgggaa ctggtggatg aagaaaggaa gctgcaccaa
    15901 caaggcaggt gtcggacttg tgtgtacaac atgatgggga aaagagagaa gaagctgtca
    15961 gagtttggga aagcaaaggg aagccgtgcc atatggtata tgtggctggg agcgcggtat
    16021 cttgagtttg aggccctggg attcctgaat gaggaccatt gggcttccag ggaaaactca
    16081 ggaggaggag tggaaggcat tggcttacaa tacctaggat atgtgatcag agacctggct
    16141 gcaatggatg gtggtggatt ctacgcggat gacaccgctg gatgggacac gcgcatcaca
    16201 gaggcagacc ttgatgatga acaggagatc ttgaactaca tgagcccaca tcacaaaaaa
    16261 ctggcacaag cagtgatgga aatgacatac aagaacaaag tggtgaaagt gttgagacca
    16321 gccccaggag ggaaagccta catggatgtc ataagtcgac gagaccagag aggatccggg
    16381 caggtagtga cttatgctct gaacaccatc accaacttga aagtccaatt gatcagaatg
    16441 gcagaagcag agatggtgat acatcaccaa catgttcaag attgtgatga atcagttctg
    16501 accaggctgg aggcatggct cactgagcac ggatgtgaca gactgaagag gatggcggtg
    16561 agtggagacg actgtgtggt ccggcccatc gatgacaggt tcggcctggc cctgtcccat
    16621 ctcaacgcca tgtccaaggt tagaaaggac atatctgaat ggcagccatc aaaagggtgg
    16681 aatgattggg agaatgtgcc cttctgttcc caccacttcc atgaactaca gctgaaggat
    16741 ggcaggagga ttgtggtgcc ttgccgagaa caggacgagc tcattgggag aggaagggtg
    16801 tctccaggaa acggctggat gatcaaggaa acagcttgcc tcagcaaagc ctatgccaac
    16861 atgtggtcac tgatgtattt tcacaaaagg gacatgaggc tactgtcatt ggctgtttcc
    16921 tcagctgttc ccacctcatg ggttccacaa ggacgcacaa catggtcgat tcatgggaaa
    16981 ggggagtgga tgaccacgga agacatgctt gaggtgtgga acagagtatg gataaccaac
    17041 aacccacaca tgcaggacaa gacaatggtg aaaaaatgga gagatgtccc ttatctaacc
    17101 aagagacaag acaagctgtg cggatcactg attggaatga ccaatagggc cacctgggcc
    17161 tcccacatcc atttagtcat ccatcgtatc cgaacgctga ttggacagga gaaatacact
    17221 gactacctaa cagtcatgga caggtattct gtggatgctg acctgcaact gggtgagctt
    17281 atctgaaaca ccatctaaca ggaataaccg ggatacaaac cacgggtgga gaaccggact
    17341 ccccacaacc tgaaaccggg atataaacca cggctggaga accggactcc gcacttaaaa
    17401 tgaaacagaa accgggataa aaactacgga tggagaaccg gactccacac attgagacag
    17461 aagaagttgt cagcccagaa ccccacacga gttttgccac tgctaagctg tgaggcagtg
    17521 caggctggga cagccgacct ccaggttgcg aaaaacctgg tttctgggac ctcccacccc
    17581 agagtaaaaa gaacggagcc tccgctacca ccctcccacg tggtggtaga aagacggggt
    17641 ctagaggtta gaggagaccc tccagggaac aaatagtggg accatattga cgccagggaa
    17701 agaccggagt ggttctctgc ttttcctcca gaggtctgtg agcacagttt gctcaagaat
    17761 aagcagacct ttggatgaca aacacaaaac cactcgagca agacgtttcc cgttgaatat
    17821 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
    17881 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
    17941 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
    18001 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
    18061 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg
    18121 agtcagcaac accttcttca cgaggcagac ctcagcgcta gcggagtgta gtgggatccc
    18181 cgggtaccga gctcgaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
    18241 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    18301 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    18361 ttgcgcagct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
    18421 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    18481 gccagccccg acacccgcca acacccgctg acgcgaaccc cttgcggcc
//
