LOCUS       sequence-366326-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-R2505P
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             134..928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(188..207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     798..817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1102..1223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1139..1158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1193..1212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1272..1288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1272..1288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1285..1307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1296..1312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1320..1326,1327..1344,1345..1350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1365..1386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1503..1520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1674..2262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1754..1773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2433..3224,3225..3293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3056..3075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3294..3398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3473..3492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3664..4043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4044..4247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4198..4218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4292..4311
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4292..4310
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4396..4405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7277..7286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10486..10764
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             10498..10770
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(12185..12201)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12185..12201)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(12236..12254)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12237..12254)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12274..12291)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12280..12504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12550..12978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12637..12656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12847..12868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12987..13007)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12992..13254,1..67)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13172..13254,1..53)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13234..13253
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
      121 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
      181 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
      241 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
      301 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
      361 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
      421 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
      481 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
      541 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
      601 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
      661 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
      721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
      781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
      841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
      901 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
      961 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     1021 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     1081 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     1141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1201 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     1261 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2221 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2281 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2341 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2401 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2461 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2521 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2581 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2641 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2701 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2761 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2821 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2881 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2941 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3001 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3061 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3121 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3181 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3241 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3301 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3361 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3421 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     3481 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     3541 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     3601 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     3661 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     3721 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3781 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3841 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3901 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3961 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4021 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4081 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4141 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4201 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     4261 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     4321 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tctgcagata
     4381 tccatcacac tggcggccgc catggattat aaagatgatg atgatatgct tggaaccgga
     4441 cctgccgccg ccaccaccgc tgccaccaca tctagcaatg tgagcgtcct gcagcagttt
     4501 gccagtggcc taaagagccg gaatgaggaa accagggcca aagccgccaa ggagctccag
     4561 cactatgtca ccatggaact ccgagagatg agtcaagagg agtctactcg cttctatgac
     4621 caactgaacc atcacatttt tgaattggtt tccagctcag atgccaatga gaggaaaggt
     4681 ggcatcttgg ccatagctag cctcatagga gtggaaggtg ggaatgccac ccgaattggc
     4741 agatttgcca actatcttcg gaacctcctc ccctccaatg acccagttgt catggaaatg
     4801 gcatccaagg ccattggccg tcttgccatg gcaggggaca cttttaccgc tgagtacgtg
     4861 gaatttgagg tgaagcgagc cctggaatgg ctgggtgctg accgcaatga gggccggaga
     4921 catgcagctg tcctggttct ccgtgagctg gccatcagcg tccctacctt cttcttccag
     4981 caagtgcaac ccttctttga caacattttt gtggccgtgt gggaccccaa acaggccatc
     5041 cgtgagggag ctgtagccgc ccttcgtgcc tgtctgattc tcacaaccca gcgtgagccg
     5101 aaggagatgc agaagcctca gtggtacagg cacacatttg aagaagcaga gaagggattt
     5161 gatgagacct tggccaaaga gaagggcatg aatcgggatg atcggatcca tggagccttg
     5221 ttgatcctta acgagctggt ccgaatcagc agcatggagg gagagcgtct gagagaagaa
     5281 atggaagaaa tcacacagca gcagctggta cacgacaagt actgcaaaga tctcatgggc
     5341 ttcggaacaa aacctcgtca cattaccccc ttcaccagtt tccaggctgt acagccccag
     5401 cagtcaaatg ccttggtggg gctgctgggg tacagctctc accaaggcct catgggattt
     5461 gggacctccc ccagtccagc taagtccacc ctggtggaga gccggtgttg cagagacttg
     5521 atggaggaga aatttgatca ggtgtgccag tgggtgctga aatgcaggaa tagcaagaac
     5581 tcgctgatcc aaatgacaat ccttaatttg ttgccccgct tggctgcatt ccgaccttct
     5641 gccttcacag atacccagta tctccaagat accatgaacc atgtcctaag ctgtgtcaag
     5701 aaggagaagg aacgtacagc ggccttccaa gccctggggc tactttctgt ggctgtgagg
     5761 tctgagttta aggtctattt gcctcgcgtg ctggacatca tccgagcggc cctgccccca
     5821 aaggacttcg cccataagag gcagaaggca atgcaggtgg acgccacagt cttcacttgc
     5881 atcagcatgc tggctcgagc aatggggcca ggcatccagc aggatatcaa ggagctgctg
     5941 gagcccatgc tggcagtggg actaagccct gccctcactg cagtgctcta cgacctgagc
     6001 cgtcagattc cacagctaaa gaaggacatt caagatgggc tactgaaaat gctgtccctg
     6061 gtccttatgc acaaacccct tcgccaccca ggcatgccca agggcctggc ccatcagctg
     6121 gcctctcctg gcctcacgac cctccctgag gccagcgatg tgggcagcat cactcttgcc
     6181 ctccgaacgc ttggcagctt tgaatttgaa ggccactctc tgacccaatt tgttcgccac
     6241 tgtgcggatc atttcctgaa cagtgagcac aaggagatcc gcatggaggc tgcccgcacc
     6301 tgctcccgcc tgctcacacc ctccatccac ctcatcagtg gccatgctca tgtggttagc
     6361 cagaccgcag tgcaagtggt ggcagatgtg cttagcaaac tgctcgtagt tgggataaca
     6421 gatcctgacc ctgacattcg ctactgtgtc ttggcgtccc tggacgagcg ctttgatgca
     6481 cacctggccc aggcggagaa cttgcaggcc ttgtttgtgg ctctgaatga ccaggtgttt
     6541 gagatccggg agctggccat ctgcactgtg ggccgactca gtagcatgaa ccctgccttt
     6601 gtcatgcctt tcctgcgcaa gatgctcatc cagattttga cagagttgga gcacagtggg
     6661 attggaagaa tcaaagagca gagtgcccgc atgctggggc acctggtctc caatgccccc
     6721 cgactcatcc gcccctacat ggagcctatt ctgaaggcat taattttgaa actgaaagat
     6781 ccagaccctg atccaaaccc aggtgtgatc aataatgtcc tggcaacaat aggagaattg
     6841 gcacaggtta gtggcctgga aatgaggaaa tgggttgatg aactttttat tatcatcatg
     6901 gacatgctcc aggattcctc tttgttggcc aaaaggcagg tggctctgtg gaccctggga
     6961 cagttggtgg ccagcactgg ctatgtagta gagccctaca ggaagtaccc tactttgctt
     7021 gaggtgctac tgaattttct gaagactgag cagaaccagg gtacacgcag agaggccatc
     7081 cgtgtgttag ggcttttagg ggctttggat ccttacaagc acaaagtgaa cattggcatg
     7141 atagaccagt cccgggatgc ctctgctgtc agcctgtcag aatccaagtc aagtcaggat
     7201 tcctctgact atagcactag tgaaatgctg gtcaacatgg gaaacttgcc tctggatgag
     7261 ttctacccag ctgtgtccat ggtggccctg atgcggatct tccgagacca gtcactctct
     7321 catcatcaca ccatggttgt ccaggccatc accttcatct tcaagtccct gggactcaaa
     7381 tgtgtgcagt tcctgcccca ggtcatgccc acgttcctta atgtcattcg agtctgtgat
     7441 ggggccatcc gggaattttt gttccagcag ctgggaatgt tggtgtcctt tgtgaagagc
     7501 cacatcagac cttatatgga tgaaatagtc accctcatga gagaattctg ggtcatgaac
     7561 acctcaattc agagcacgat cattcttctc attgagcaaa ttgtggtagc tcttgggggt
     7621 gaatttaagc tctacctgcc ccagctgatc ccacacatgc tgcgtgtctt catgcatgac
     7681 aacagcccag gccgcattgt ctctatcaag ttactggctg caatccagct gtttggcgcc
     7741 aacctggatg actacctgca tttactgctg cctcctattg ttaagttgtt tgatgcccct
     7801 gaagctccac tgccatctcg aaaggcagcg ctagagactg tggaccgcct gacggagtcc
     7861 ctggatttca ctgactatgc ctcccggatc attcacccta ttgttcgaac actggaccag
     7921 agcccagaac tgcgctccac agccatggac acgctgtctt cacttgtttt tcagctgggg
     7981 aagaagtacc aaattttcat tccaatggtg aataaagttc tggtgcgaca ccgaatcaat
     8041 catcagcgct atgatgtgct catctgcaga attgtcaagg gatacacact tgctgatgaa
     8101 gaggaggatc ctttgattta ccagcatcgg atgcttagga gtggccaagg ggatgcattg
     8161 gctagtggac cagtggaaac aggacccatg aagaaactgc acgtcagcac catcaacctc
     8221 caaaaggcct ggggcgctgc caggagggtc tccaaagatg actggctgga atggctgaga
     8281 cggctgagcc tggagctgct gaaggactca tcatcgccct ccctgcgctc ctgctgggcc
     8341 ctggcacagg cctacaaccc gatggccagg gatctcttca atgctgcatt tgtgtcctgc
     8401 tggtctgaac tgaatgaaga tcaacaggat gagctcatca gaagcatcga gttggccctc
     8461 acctcacaag acatcgctga agtcacacag accctcttaa acttggctga attcatggaa
     8521 cacagtgaca agggccccct gccactgaga gatgacaatg gcattgttct gctgggtgag
     8581 agagctgcca agtgccgagc atatgccaaa gcactacact acaaagaact ggagttccag
     8641 aaaggcccca cccctgccat tctagaatct ctcatcagca ttaataataa gctacagcag
     8701 ccggaggcag cggccggagt gttagaatat gccatgaaac actttggaga gctggagatc
     8761 caggctacct ggtatgagaa actgcacgag tgggaggatg cccttgtggc ctatgacaag
     8821 aaaatggaca ccaacaagga cgacccagag ctgatgctgg gccgcatgcg ctgcctcgag
     8881 gccttggggg aatggggtca actccaccag cagtgctgtg aaaagtggac cctggttaat
     8941 gatgagaccc aagccaagat ggcccggatg gctgctgcag ctgcatgggg tttaggtcag
     9001 tgggacagca tggaagaata cacctgtatg atccctcggg acacccatga tggggcattt
     9061 tatagagctg tgctggcact gcatcaggac ctcttctcct tggcacaaca gtgcattgac
     9121 aaggccaggg acctgctgga tgctgaatta actgcaatgg caggagagag ttacagtcgg
     9181 gcatatgggg ccatggtttc ttgccacatg ctgtccgagc tggaggaggt tatccagtac
     9241 aaacttgtcc ccgagcgacg agagatcatc cgccagatct ggtgggagag actgcagggc
     9301 tgccagcgta tcgtagagga ctggcagaaa atccttatgg tgcggtccct tgtggtcagc
     9361 cctcatgaag acatgagaac ctggctcaag tatgcaagcc tgtgcggcaa gagtggcagg
     9421 ctggctcttg ctcataaaac tttagtgttg ctcctgggag ttgatccgtc tcggcaactt
     9481 gaccatcctc tgccaacagt tcaccctcag gtgacctatg cctacatgaa aaacatgtgg
     9541 aagagtgccc gcaagatcga tgccttccag cacatgcagc attttgtcca gaccatgcag
     9601 caacaggccc agcatgccat cgctactgag gaccagcagc ataagcagga actgcacaag
     9661 ctcatggccc gatgcttcct gaaacttgga gagtggcagc tgaatctaca gggcatcaat
     9721 gagagcacaa tccccaaagt gctgcagtac tacagcgccg ccacagagca cgaccgcagc
     9781 tggtacaagg cctggcatgc gtgggcagtg atgaacttcg aagctgtgct acactacaaa
     9841 catcagaacc aagcccgcga tgagaagaag aaactgcgtc atgccagcgg ggccaacatc
     9901 accaacgcca ccactgccgc caccacggcc gccactgcca ccaccactgc cagcaccgag
     9961 ggcagcaaca gtgagagcga ggccgagagc accgagaaca gccccacccc atcgccgctg
    10021 cagaagaagg tcactgagga tctgtccaaa accctcctga tgtacacggt gcctgccgtc
    10081 cagggcttct tccgttccat ctccttgtca cgaggcaaca acctccagga tacactcaga
    10141 gttctcacct tatggtttga ttatggtcac tggccagatg tcaatgaggc cttagtggag
    10201 ggggtgaaag ccatccagat tgatacctgg ctacaggtta tacctcagct cattgcaaga
    10261 attgatacgc ccagaccctt ggtgggacgt ctcattcacc agcttctcac agacattggt
    10321 cggtaccacc cccaggccct catctaccca ctgacagtgg cttctaagtc taccacgaca
    10381 gcccggcaca atgcagccaa caagattctg aagaacatgt gtgagcacag caacaccctg
    10441 gtccagcagg ccatgatggt gagcgaggag ctgatccgag tggccatcct ctggcatgag
    10501 atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa
    10561 ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg
    10621 aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc
    10681 aggaagtaca tgaaatcagg gaatgtcaag gacctcaccc aagcctggga cctctattat
    10741 catgtgttcc gacgaatctc aaagcagctg cctcagctca catccttaga gctgcaatat
    10801 gtttccccaa aacttctgat gtgccgggac cttgaattgg ctgtgccagg aacatatgac
    10861 cccaaccagc caatcattcg cattcagtcc atagcaccgt ctttgcaagt catcacatcc
    10921 aagcagaggc cccggaaatt gacacttatg ggcagcaacg gacatgagtt tgttttcctt
    10981 ctaaaaggcc atgaagatct gcgccaggat gagcgtgtga tgcagctctt cggcctggtt
    11041 aacacccttc tggccaatga cccaacatct cttcggaaaa acctcagcat ccagagatac
    11101 gctgtcatcc ctttatcgac caactcgggc ctcattggct gggttcccca ctgtgacaca
    11161 ctgcacgccc tcatccggga ctacagggag aagaagaaga tccttctcaa catcgagcat
    11221 cgcatcatgt tgcggatggc tccggactat gaccacttga ctctgatgca gaaggtggag
    11281 gtgtttgagc atgccgtcaa taatacagct ggggacgacc tggccaagct gctgtggctg
    11341 aaaagcccca gctccgaggt gtggtttgac cgaagaacca attatacccg ttctttagcg
    11401 gtcatgtcaa tggttgggta tattttaggc ctgggagata gacacccatc caacctgatg
    11461 ctggaccgtc tgagtgggaa gatcctgcac attgactttg gggactgctt tgaggttgct
    11521 atgacccgag agaagtttcc agagaagatt ccatttagac taacaagaat gttgaccaat
    11581 gctatggagg ttacaggcct ggatggcaac tacagaatca catgccacac agtgatggag
    11641 gtgctgcgag agcacaagga cagtgtcatg gccgtgctgg aagcctttgt ctatgacccc
    11701 ttgctgaact ggaggctgat ggacacaaat accaaaggca acaagcgatc ccgaacgagg
    11761 acggattcct actctgctgg ccagtcagtc gaaattttgg acggtgtgga acttggagag
    11821 ccagcccata agaaaacggg gaccacagtg ccagaatcta ttcattcttt cattggagac
    11881 ggtttggtga aaccagaggc cctaaataag aaagctatcc agattattaa cagggttcca
    11941 gataagctca ctggtcggga cttctctcat gatgacactt tggatgttcc aacgcaagtt
    12001 gagctgctca tcaaacaagc gacatcccat gaaaacctct gccagtgcta tattggctgg
    12061 tgccctttct ggtaactgga ggcccagatg tgcccatcac gttttttctg aggcttttgt
    12121 actttagtaa atgcttccac taaactgaaa ccatggtgag aaagtttgac tttgttaaat
    12181 gggggatcca ctagttctag agcggccgct cgagcatgca tctagagggc cctattctat
    12241 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
    12301 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
    12361 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
    12421 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
    12481 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
    12541 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    12601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    12661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    12721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    12781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    12841 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    12901 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    12961 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    13021 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    13081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    13141 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    13201 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggc
//
