LOCUS       sequence-366327-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-V2006I
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             176..970
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(230..249)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     840..859
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1144..1265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1181..1200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1235..1254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1314..1330)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1314..1330)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1327..1349)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1338..1354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1362..1368,1369..1386,1387..1392))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1407..1428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1545..1562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1716..2304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1796..1815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2475..3266,3267..3335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3098..3117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3336..3440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3515..3534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3706..4085
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4086..4289
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4240..4260
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4334..4353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4334..4352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4438..4447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7319..7328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10528..10806
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             10540..10812
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(12227..12243)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12227..12243)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(12278..12296)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12279..12296)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12316..12333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12322..12546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12592..13020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12679..12698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12889..12910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13029..13049)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(13034..13254,1..109)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13214..13254,1..95)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
       61 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      121 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
      181 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      241 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      301 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
      361 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      421 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      481 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      541 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      601 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      661 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
      721 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      781 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      841 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
      901 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
      961 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1021 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1081 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1141 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1261 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     1321 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1381 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1441 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1501 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1981 cccgttcagc acgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2341 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2401 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3061 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     3481 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3541 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     3601 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     3661 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     3721 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3781 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3841 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3901 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3961 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4021 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4081 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4141 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4201 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4261 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     4321 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
     4381 tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc
     4441 gccatggatt ataaagatga tgatgatatg cttggaaccg gacctgccgc cgccaccacc
     4501 gctgccacca catctagcaa tgtgagcgtc ctgcagcagt ttgccagtgg cctaaagagc
     4561 cggaatgagg aaaccagggc caaagccgcc aaggagctcc agcactatgt caccatggaa
     4621 ctccgagaga tgagtcaaga ggagtctact cgcttctatg accaactgaa ccatcacatt
     4681 tttgaattgg tttccagctc agatgccaat gagaggaaag gtggcatctt ggccatagct
     4741 agcctcatag gagtggaagg tgggaatgcc acccgaattg gcagatttgc caactatctt
     4801 cggaacctcc tcccctccaa tgacccagtt gtcatggaaa tggcatccaa ggccattggc
     4861 cgtcttgcca tggcagggga cacttttacc gctgagtacg tggaatttga ggtgaagcga
     4921 gccctggaat ggctgggtgc tgaccgcaat gagggccgga gacatgcagc tgtcctggtt
     4981 ctccgtgagc tggccatcag cgtccctacc ttcttcttcc agcaagtgca acccttcttt
     5041 gacaacattt ttgtggccgt gtgggacccc aaacaggcca tccgtgaggg agctgtagcc
     5101 gcccttcgtg cctgtctgat tctcacaacc cagcgtgagc cgaaggagat gcagaagcct
     5161 cagtggtaca ggcacacatt tgaagaagca gagaagggat ttgatgagac cttggccaaa
     5221 gagaagggca tgaatcggga tgatcggatc catggagcct tgttgatcct taacgagctg
     5281 gtccgaatca gcagcatgga gggagagcgt ctgagagaag aaatggaaga aatcacacag
     5341 cagcagctgg tacacgacaa gtactgcaaa gatctcatgg gcttcggaac aaaacctcgt
     5401 cacattaccc ccttcaccag tttccaggct gtacagcccc agcagtcaaa tgccttggtg
     5461 gggctgctgg ggtacagctc tcaccaaggc ctcatgggat ttgggacctc ccccagtcca
     5521 gctaagtcca ccctggtgga gagccggtgt tgcagagact tgatggagga gaaatttgat
     5581 caggtgtgcc agtgggtgct gaaatgcagg aatagcaaga actcgctgat ccaaatgaca
     5641 atccttaatt tgttgccccg cttggctgca ttccgacctt ctgccttcac agatacccag
     5701 tatctccaag ataccatgaa ccatgtccta agctgtgtca agaaggagaa ggaacgtaca
     5761 gcggccttcc aagccctggg gctactttct gtggctgtga ggtctgagtt taaggtctat
     5821 ttgcctcgcg tgctggacat catccgagcg gccctgcccc caaaggactt cgcccataag
     5881 aggcagaagg caatgcaggt ggacgccaca gtcttcactt gcatcagcat gctggctcga
     5941 gcaatggggc caggcatcca gcaggatatc aaggagctgc tggagcccat gctggcagtg
     6001 ggactaagcc ctgccctcac tgcagtgctc tacgacctga gccgtcagat tccacagcta
     6061 aagaaggaca ttcaagatgg gctactgaaa atgctgtccc tggtccttat gcacaaaccc
     6121 cttcgccacc caggcatgcc caagggcctg gcccatcagc tggcctctcc tggcctcacg
     6181 accctccctg aggccagcga tgtgggcagc atcactcttg ccctccgaac gcttggcagc
     6241 tttgaatttg aaggccactc tctgacccaa tttgttcgcc actgtgcgga tcatttcctg
     6301 aacagtgagc acaaggagat ccgcatggag gctgcccgca cctgctcccg cctgctcaca
     6361 ccctccatcc acctcatcag tggccatgct catgtggtta gccagaccgc agtgcaagtg
     6421 gtggcagatg tgcttagcaa actgctcgta gttgggataa cagatcctga ccctgacatt
     6481 cgctactgtg tcttggcgtc cctggacgag cgctttgatg cacacctggc ccaggcggag
     6541 aacttgcagg ccttgtttgt ggctctgaat gaccaggtgt ttgagatccg ggagctggcc
     6601 atctgcactg tgggccgact cagtagcatg aaccctgcct ttgtcatgcc tttcctgcgc
     6661 aagatgctca tccagatttt gacagagttg gagcacagtg ggattggaag aatcaaagag
     6721 cagagtgccc gcatgctggg gcacctggtc tccaatgccc cccgactcat ccgcccctac
     6781 atggagccta ttctgaaggc attaattttg aaactgaaag atccagaccc tgatccaaac
     6841 ccaggtgtga tcaataatgt cctggcaaca ataggagaat tggcacaggt tagtggcctg
     6901 gaaatgagga aatgggttga tgaacttttt attatcatca tggacatgct ccaggattcc
     6961 tctttgttgg ccaaaaggca ggtggctctg tggaccctgg gacagttggt ggccagcact
     7021 ggctatgtag tagagcccta caggaagtac cctactttgc ttgaggtgct actgaatttt
     7081 ctgaagactg agcagaacca gggtacacgc agagaggcca tccgtgtgtt agggctttta
     7141 ggggctttgg atccttacaa gcacaaagtg aacattggca tgatagacca gtcccgggat
     7201 gcctctgctg tcagcctgtc agaatccaag tcaagtcagg attcctctga ctatagcact
     7261 agtgaaatgc tggtcaacat gggaaacttg cctctggatg agttctaccc agctgtgtcc
     7321 atggtggccc tgatgcggat cttccgagac cagtcactct ctcatcatca caccatggtt
     7381 gtccaggcca tcaccttcat cttcaagtcc ctgggactca aatgtgtgca gttcctgccc
     7441 caggtcatgc ccacgttcct taatgtcatt cgagtctgtg atggggccat ccgggaattt
     7501 ttgttccagc agctgggaat gttggtgtcc tttgtgaaga gccacatcag accttatatg
     7561 gatgaaatag tcaccctcat gagagaattc tgggtcatga acacctcaat tcagagcacg
     7621 atcattcttc tcattgagca aattgtggta gctcttgggg gtgaatttaa gctctacctg
     7681 ccccagctga tcccacacat gctgcgtgtc ttcatgcatg acaacagccc aggccgcatt
     7741 gtctctatca agttactggc tgcaatccag ctgtttggcg ccaacctgga tgactacctg
     7801 catttactgc tgcctcctat tgttaagttg tttgatgccc ctgaagctcc actgccatct
     7861 cgaaaggcag cgctagagac tgtggaccgc ctgacggagt ccctggattt cactgactat
     7921 gcctcccgga tcattcaccc tattgttcga acactggacc agagcccaga actgcgctcc
     7981 acagccatgg acacgctgtc ttcacttgtt tttcagctgg ggaagaagta ccaaattttc
     8041 attccaatgg tgaataaagt tctggtgcga caccgaatca atcatcagcg ctatgatgtg
     8101 ctcatctgca gaattgtcaa gggatacaca cttgctgatg aagaggagga tcctttgatt
     8161 taccagcatc ggatgcttag gagtggccaa ggggatgcat tggctagtgg accagtggaa
     8221 acaggaccca tgaagaaact gcacgtcagc accatcaacc tccaaaaggc ctggggcgct
     8281 gccaggaggg tctccaaaga tgactggctg gaatggctga gacggctgag cctggagctg
     8341 ctgaaggact catcatcgcc ctccctgcgc tcctgctggg ccctggcaca ggcctacaac
     8401 ccgatggcca gggatctctt caatgctgca tttgtgtcct gctggtctga actgaatgaa
     8461 gatcaacagg atgagctcat cagaagcatc gagttggccc tcacctcaca agacatcgct
     8521 gaagtcacac agaccctctt aaacttggct gaattcatgg aacacagtga caagggcccc
     8581 ctgccactga gagatgacaa tggcattgtt ctgctgggtg agagagctgc caagtgccga
     8641 gcatatgcca aagcactaca ctacaaagaa ctggagttcc agaaaggccc cacccctgcc
     8701 attctagaat ctctcatcag cattaataat aagctacagc agccggaggc agcggccgga
     8761 gtgttagaat atgccatgaa acactttgga gagctggaga tccaggctac ctggtatgag
     8821 aaactgcacg agtgggagga tgcccttgtg gcctatgaca agaaaatgga caccaacaag
     8881 gacgacccag agctgatgct gggccgcatg cgctgcctcg aggccttggg ggaatggggt
     8941 caactccacc agcagtgctg tgaaaagtgg accctggtta atgatgagac ccaagccaag
     9001 atggcccgga tggctgctgc agctgcatgg ggtttaggtc agtgggacag catggaagaa
     9061 tacacctgta tgatccctcg ggacacccat gatggggcat tttatagagc tgtgctggca
     9121 ctgcatcagg acctcttctc cttggcacaa cagtgcattg acaaggccag ggacctgctg
     9181 gatgctgaat taactgcaat ggcaggagag agttacagtc gggcatatgg ggccatggtt
     9241 tcttgccaca tgctgtccga gctggaggag gttatccagt acaaacttgt ccccgagcga
     9301 cgagagatca tccgccagat ctggtgggag agactgcagg gctgccagcg tatcgtagag
     9361 gactggcaga aaatccttat ggtgcggtcc cttgtggtca gccctcatga agacatgaga
     9421 acctggctca agtatgcaag cctgtgcggc aagagtggca ggctggctct tgctcataaa
     9481 actttagtgt tgctcctggg agttgatccg tctcggcaac ttgaccatcc tctgccaaca
     9541 gttcaccctc aggtgaccta tgcctacatg aaaaacatgt ggaagagtgc ccgcaagatc
     9601 gatgccttcc agcacatgca gcattttgtc cagaccatgc agcaacaggc ccagcatgcc
     9661 atcgctactg aggaccagca gcataagcag gaactgcaca agctcatggc ccgatgcttc
     9721 ctgaaacttg gagagtggca gctgaatcta cagggcatca atgagagcac aatccccaaa
     9781 gtgctgcagt actacagcgc cgccacagag cacgaccgca gctggtacaa ggcctggcat
     9841 gcgtgggcag tgatgaactt cgaagctgtg ctacactaca aacatcagaa ccaagcccgc
     9901 gatgagaaga agaaactgcg tcatgccagc ggggccaaca tcaccaacgc caccactgcc
     9961 gccaccacgg ccgccactgc caccaccact gccagcaccg agggcagcaa cagtgagagc
    10021 gaggccgaga gcaccgagaa cagccccacc ccatcgccgc tgcagaagaa ggtcactgag
    10081 gatctgtcca aaaccctcct gatgtacacg gtgcctgccg tccagggctt cttccgttcc
    10141 atctccttgt cacgaggcaa caacctccag gatacactca gagttctcac cttatggttt
    10201 gattatggtc actggccaga tgtcaatgag gccttagtgg agggggtgaa agccatccag
    10261 attgatacct ggctacaggt tatacctcag ctcattgcaa gaattgatac gcccagaccc
    10321 ttggtgggac gtctcattca ccagcttctc acagacattg gtcggtacca cccccaggcc
    10381 ctcatctacc cactgacagt ggcttctaag tctaccacga cagcccggca caatgcagcc
    10441 aacaagattc tgaagaacat gtgtgagcac agcaacaccc tgatccagca ggccatgatg
    10501 gtgagcgagg agctgatccg agtggccatc ctctggcatg agatgtggca tgaaggcctg
    10561 gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg
    10621 gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat
    10681 caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca
    10741 gggaatgtca aggacctcac ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc
    10801 tcaaagcagc tgcctcagct cacatcctta gagctgcaat atgtttcccc aaaacttctg
    10861 atgtgccggg accttgaatt ggctgtgcca ggaacatatg accccaacca gccaatcatt
    10921 cgcattcagt ccatagcacc gtctttgcaa gtcatcacat ccaagcagag gccccggaaa
    10981 ttgacactta tgggcagcaa cggacatgag tttgttttcc ttctaaaagg ccatgaagat
    11041 ctgcgccagg atgagcgtgt gatgcagctc ttcggcctgg ttaacaccct tctggccaat
    11101 gacccaacat ctcttcggaa aaacctcagc atccagagat acgctgtcat ccctttatcg
    11161 accaactcgg gcctcattgg ctgggttccc cactgtgaca cactgcacgc cctcatccgg
    11221 gactacaggg agaagaagaa gatccttctc aacatcgagc atcgcatcat gttgcggatg
    11281 gctccggact atgaccactt gactctgatg cagaaggtgg aggtgtttga gcatgccgtc
    11341 aataatacag ctggggacga cctggccaag ctgctgtggc tgaaaagccc cagctccgag
    11401 gtgtggtttg accgaagaac caattatacc cgttctttag cggtcatgtc aatggttggg
    11461 tatattttag gcctgggaga tagacaccca tccaacctga tgctggaccg tctgagtggg
    11521 aagatcctgc acattgactt tggggactgc tttgaggttg ctatgacccg agagaagttt
    11581 ccagagaaga ttccatttag actaacaaga atgttgacca atgctatgga ggttacaggc
    11641 ctggatggca actacagaat cacatgccac acagtgatgg aggtgctgcg agagcacaag
    11701 gacagtgtca tggccgtgct ggaagccttt gtctatgacc ccttgctgaa ctggaggctg
    11761 atggacacaa ataccaaagg caacaagcga tcccgaacga ggacggattc ctactctgct
    11821 ggccagtcag tcgaaatttt ggacggtgtg gaacttggag agccagccca taagaaaacg
    11881 gggaccacag tgccagaatc tattcattct ttcattggag acggtttggt gaaaccagag
    11941 gccctaaata agaaagctat ccagattatt aacagggttc gagataagct cactggtcgg
    12001 gacttctctc atgatgacac tttggatgtt ccaacgcaag ttgagctgct catcaaacaa
    12061 gcgacatccc atgaaaacct ctgccagtgc tatattggct ggtgcccttt ctggtaactg
    12121 gaggcccaga tgtgcccatc acgttttttc tgaggctttt gtactttagt aaatgcttcc
    12181 actaaactga aaccatggtg agaaagtttg actttgttaa atgggggatc cactagttct
    12241 agagcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
    12301 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    12361 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    12421 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    12481 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    12541 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
    12601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    12661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    12721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    12781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    12841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    12901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    12961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    13021 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    13081 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    13141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    13201 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgcc
//
