LOCUS       sequence-366333-        9285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expression cre-dependent 
            Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  TITLE     AAV vector using mDlx promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             839..1014
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1077..1276
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1477..1893
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1823..1845
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1861..1877
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     intron          2606..2738
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2721..2740
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    complement(2799..2832)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2883..2916)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(2927..3640,3641..3643,3644..3646))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2930..3640,3641..3643,3644..3646))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2972..2993)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3321..3340
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3580..3601
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4595..4648)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5464..5487)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5662..5681)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5931..5949
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    6100..6133
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6184..6217
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6227..6815
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6280..6300)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6698..6709)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7010..7479
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7609..7626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7780..8368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7860..7879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8528..9285,1..34,35..103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9151..9170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
       61 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
      121 gatccggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca
      841 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      901 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
      961 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
     1021 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     1081 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     1141 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1201 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1261 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1441 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1501 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1561 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1621 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1681 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1741 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1801 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1861 ctttatccag ccctcactcc ttctctaggc gccggaattc gatgtcgacg aattctatac
     1921 actcacagtg gtttggcata tatttggtga aattttttaa ggaaaaatta gtgttggttt
     1981 cgatatatgg tagctttttc tctaacataa tttgaataat tcagcaaagc cctactacca
     2041 gctgtacttc tgcagcctct tccattcttt ccagcattat aattttggtt aattttcaat
     2101 tttaggtcct acgtctctgc aatttgtgta tgaataacag aataatttcc ctcttttgtt
     2161 tcgcctttcc tgttcctgaa tctaaataaa gatggctttt tagtattaaa agtggaagaa
     2221 aattacaggt aattatcttt gacggtaaaa acgctgtaat cagcgggcta catgaaaaat
     2281 tactctaatt atggctgcat ttaagagaat ggaaaaaaac cttcttgtgg ataaaaacct
     2341 taaattgtcc ccaatgtctg cttcaaattg gatggcactg cagctggagg ctttgttcag
     2401 aattgatcct ggggagctac gaacccaaag tttcacagta ggaagcttga tatcgaattc
     2461 ctgcagcccg ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc
     2521 tagcctgcag gtcgaggagc gcagccttcc agaagcagag cgcggcgcct taagctgcag
     2581 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     2641 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     2701 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     2761 cagctcttaa gaattcgaaa tataacatcg gatccggaat aacttcgtat aggatacttt
     2821 atacgaagtt atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
     2881 taataacttc gtatagcata cattatacga agttatttaa ttaagcttac ttgtacagct
     2941 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     3001 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     3061 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     3121 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     3181 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     3241 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     3301 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     3361 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     3421 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     3481 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     3541 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     3601 ggatgggcac caccccggtg aacagctcct cgcccttgct caccattcgc gacatctgat
     3661 tggagaagga ggtgggcgca ccctgttggc cgtagccttg ctgcccatag tcgccctgag
     3721 gcccgtagcc accgccaccg ccgctggctg gctgcccgta atcgggttga taaccaccct
     3781 gaggcccgta ggagtcctgg ggcccatagc ctccggggcc ctgcccatag cccgcatcgc
     3841 cgtaggcatc gccaggagct ggttgctttt ctggggcgcc tggaggtgcg cgcatgaatg
     3901 gggcggccca gcctgtctcc ttgaacacga accataggtt gccaacccag agcaccaggt
     3961 tcaggaagcc aaacaccacc gaggtgttga gtcctgaagt cacagggtcc ctcagttcct
     4021 tgcatgtgtt tcctgtctgg cggcacatag gcatctcctt gataatgttc tctgggtcag
     4081 tggccatctt cacatcggac aggcctttgg cccaggcgga tgagctaact agccacatga
     4141 aagcgaacac tgctgtggcc aggaagtcca tcattggccc tttgttgttc tctcggtact
     4201 tgttctgcag gaagatgtag gtggccaggg cccccatgga gtagaggaag gcaaacacag
     4261 ccacggtgac aaagaattca gccgaggagg agtagtcacc aactaggaag atcttggtag
     4321 tgcccccttt aacgcaggag ggtgcatcaa agtacacttg gtgcagcctg aatgggtact
     4381 caaattcgac ttcgatgttg agggcactct ccgtcttgtt ggcacactcc acgctcagcc
     4441 gaagctctcc ggtgtagctg ccgcacgtag caaaggcgaa gatggcaaag acccactgca
     4501 gcaccttcac gaagccaagg ggctccttga ccacccggaa ctgaccccca gccaccagct
     4561 gattcaccac gtccattcta gatgagcctg gggcagggcc gggattctct tccacgtctc
     4621 cgcatgtcag aagacttcct ctgccctcac tagtcttgta cagctcgtcc atgccgtaca
     4681 gaaacagatg gtgcctcccc tcgctccgct catactgctc cacaatggtg tagtcttcgt
     4741 tatggctagt aatatccagc tttgtgtcga cgtaatagta gccaggcagc tgcacgggtt
     4801 tcttggccat gtagatggtt ttgaattcga ccagataatg ccctccatct ttcagcttca
     4861 gtgcctggtg gatctctccc ttcagcacgc cgtctctggg gtacagcctt tctgttgaag
     4921 cctcccagcc cattgtcttc ttctgcatga ctgggccatc agggggaaaa ttggtgccgc
     4981 gcattttcac cttatagatc agagtgccgt cctgcagaga gctgtcctgg gtgacagtca
     5041 ccaggccccc gtcttcgaag ttcatcactc tttcccactt aaacccctct gggaagctca
     5101 gtttcttgta atctggaatg tcagcagggt gtttcacgta ggctttactt ccatacataa
     5161 actgaggaga caggatgtcc catgcgaatg gcagagggcc gccctttgtc actttcagct
     5221 ttgctgtctg ggtgccctca tagggcctcc cttccccctc tccttcaatc tcaaactcgt
     5281 gcccgttcat gctgccctcc atcctgactt taaacctcat aaactcctta atcacggcca
     5341 tgttgttgtc ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccggtaccat
     5401 gccccaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt
     5461 cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagttgca
     5521 cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca
     5581 gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg
     5641 tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg
     5701 tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca
     5761 cggtcaccag accgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     5821 aggacagctt cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt
     5881 acatgaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     5941 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     6001 actcgtggcc gttcatggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga
     6061 cctcctcgcc cttgctcacc atactagtgg cgcgccgcga taacttcgta taaagtatcc
     6121 tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     6181 agaataactt cgtataatgt atgctatacg aagttatgtt taaaccaatc aacctctgga
     6241 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6301 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6361 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6421 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6481 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6541 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6601 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6661 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6721 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6781 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc gagcttgtta
     6841 acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     6901 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     6961 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     7021 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     7081 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     7141 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     7201 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     7261 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     7321 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     7381 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     7441 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     7501 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     7561 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7621 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7681 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7741 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7801 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7861 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7921 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7981 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8041 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8101 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8161 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8221 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8281 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8401 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8461 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     8521 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atctt
//
