LOCUS       sequence-366351-        5382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-AB1-myc
FEATURES             Location/Qualifiers
     source          1..5382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1434..1568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1458..1477)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1512..1531
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1681..1701)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1681..1699)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1720..1736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1720..1736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1733..1755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1744..1760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1768..1774,1775..1792,1793..1798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1813..1834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1951..1968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2122..2710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2202..2221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2881..3672,3673..3741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3504..3523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3742..3846)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3872..4327)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4009..4030)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4221..4240
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4398..5382
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctatgagct ttagccagga gtctggtgcc catcatcacc cccaggatta tgcaggactt
      421 tctgatgagg aggatgaaga tgatccctgc agcctaaagt cacactttta cctacagccc
      481 acacattcag tgatggggga cagtgagatg ctgagtcctt ccaagctcag ctgcactgaa
      541 agtgagagtg attcctctgg agagagtgaa ggaggtactt ctaaagactc ctcaaccacc
      601 ccaactggta gcaaagccaa aaggactctg gtaaagcctc cttactctta cattgctcta
      661 atcaccatgg ctatcctgca gagtccccac aagaaactga ctctcagtgg catctgtgat
      721 tttatcagca gcaagttccc ttactacaag gacaagttcc ctgcttggca gaattccatc
      781 aggcacaacc tgtcccttaa tgactgcttt attaaaatac cacgggaacc aggaaatccg
      841 ggaaaaggca attattggac actggaccct gcttctgagg acatgtttga taacggaagc
      901 ttccttagaa ggaggaaaag gtttaagagg caccaacagg agttctttaa ggatggactc
      961 atgatgtaca actctctgcc ttactacaga ccctacagtg ctatccaacc tcagccagtg
     1021 ctccagcaga cttcactgac atgcatggca ataccagaga ccttgcccat gtcaactcac
     1081 cttgcaccct acccagacat aaagaggaag gtttcttacc ctgcccaggg ggtacacagg
     1141 ggttttaaag cccaggatgc tgataaccat cccaataatt cacagagcaa atgttcattc
     1201 agtattgaga acatcatgag gaaacccaag gagccagagc caaatattca aagcttcaac
     1261 tcacactgga actataatca tgtattccag aggccaagtt cctgccttct cccagctgtg
     1321 cttaacttat ccactggccc tctgctagcc aatactcagg gtgccaggca atacaacctc
     1381 atacaattcc ctgggtgtta ctgatctaga actatagtga gtcgtattac gtagatccag
     1441 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1501 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1561 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1621 aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc
     1681 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1741 aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc
     1801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3841 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     3901 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3961 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4021 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4081 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4141 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4201 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4261 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4321 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4381 tcttcgctat tacgccagtc gaccgccaat tcaatatggc gtatatggac tcatgccaat
     4441 tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat
     4501 tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat
     4561 tcaatatggc ggacctggca ctgtgccaac tggggagggg tctacttggc acggtgccaa
     4621 gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc atggtgccaa
     4681 taatggcggc catattggct atatgccagg atcaatatat aggcaatatc caatatggcc
     4741 ctatgccaat atggctattg gccaggttca atactatgta ttggccctat gccatatagt
     4801 attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc ggtcccatat
     4861 accatatatg gggcttccta ataccgccca tagccactcc cccattgacg tcaatggtct
     4921 ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta tatggcgcct
     4981 cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat tgacgtcaat
     5041 aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat ccgttctcac
     5101 gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca atatctatta
     5161 atagtaactt ggcaagtaca ttactattgg aagtacgcca gggtacattg gcagtactcc
     5221 cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc ccattgacgt
     5281 caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg cggtaggcgt
     5341 gcctaatggg aggtctatat aagcaatgct cgtttaggga ac
//
