LOCUS       sequence-366371-        5104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-deltaAB
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1156..1290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1180..1199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1234..1253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1403..1423)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1403..1421)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1442..1458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1442..1458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1455..1477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1466..1482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1490..1496,1497..1514,1515..1520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1535..1556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1673..1690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1844..2432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1924..1943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2603..3394,3395..3463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3226..3245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3464..3568)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3594..4049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3731..3752)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3943..3962
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4120..5104
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctatga gctttagcca
      121 ggagtctggt gcccatcatc acccccagga ttatgcagga ctttctccct gcagcctaaa
      181 gtcacacttt tacctacagc ccacacattc agtgatgggg gacagtgaga tgctgagtcc
      241 ttccaagctc agctgcactg aaagtgagag tgattcctct ggagagagtg aaggaggtac
      301 ttctaaagac tcctcaacca ccccaactgg tagcaaagcc aaaaggactc tggtaaagcc
      361 tccttactct tacattgctc taatcaccat ggctatcctg cagagtcccc acaagaaact
      421 gactctcagt ggcatctgtg attttatcag cagcaagttc ccttactaca aggacaagtt
      481 ccctgcttgg cagaattcca tcaggcacaa cctgtccctt aatgactgct ttattaaaat
      541 accacgggaa ccaggaaatc cgggaaaagg caattattgg acactggacc ctgcttctga
      601 ggacatgttt gataacggaa gcttccttag aaggaggaaa aggtttaaga ggcaccaaca
      661 ggagttcttt aaggatggac tcatgatgta caactctctg ccttactaca gaccctacag
      721 tgctatccaa cctcagccag tgctccagca gacttcactg acatgcatgg caataccaga
      781 gaccttgccc atgtcaactc accttgcacc ctacccagac ataaagagga aggtttctta
      841 ccctgcccag ggggtacaca ggggttttaa agcccaggat gctgataacc atcccaataa
      901 ttcacagagc aaatgttcat tcagtattga gaacatcatg aggaaaccca aggagccaga
      961 gccaaatatt caaagcttca actcacactg gaactataat catgtattcc agaggccaag
     1021 ttcctgcctt ctcccagctg tgcttaactt atccactggc cctctgctag ccaatactca
     1081 gggtgccagg caatacaacc tcatacaatt ccctgggtgt tactgatcta gaactatagt
     1141 gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     1201 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1261 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1321 caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg
     1381 gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     1441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1501 ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     3601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3661 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3721 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3781 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3841 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3901 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3961 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     4021 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     4081 ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgaccgcca attcaatatg
     4141 gcgtatatgg actcatgcca attcaatatg gtggatctgg acctgtgcca attcaatatg
     4201 gcgtatatgg actcgtgcca attcaatatg gtggatctgg accccagcca attcaatatg
     4261 gcggacttgg caccatgcca attcaatatg gcggacctgg cactgtgcca actggggagg
     4321 ggtctacttg gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc aagtccgcca
     4381 tattgaattg gcatggtgcc aataatggcg gccatattgg ctatatgcca ggatcaatat
     4441 ataggcaata tccaatatgg ccctatgcca atatggctat tggccaggtt caatactatg
     4501 tattggccct atgccatata gtattccata tatgggtttt cctattgacg tagatagccc
     4561 ctcccaatgg gcggtcccat ataccatata tggggcttcc taataccgcc catagccact
     4621 cccccattga cgtcaatggt ctctatatat ggtctttcct attgacgtca tatgggcggt
     4681 cctattgacg tatatggcgc ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg
     4741 ctcaatgccc attgacgtca ataggaccac ccaccattga cgtcaatggg atggctcatt
     4801 gcccattcat atccgttctc acgcccccta ttgacgtcaa tgacggtaaa tggcccactt
     4861 ggcagtacat caatatctat taatagtaac ttggcaagta cattactatt ggaagtacgc
     4921 cagggtacat tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg
     4981 ccaagtacat ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt
     5041 gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg
     5101 gaac
//
